Molecular Nutrition

Effects of Glucocorticoids on Expression of Lipid and Bile Acid Metabolism Related Genes in Chicken Embryo Hepatocytes

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  • College of Animal Science and College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China

Received date: 2018-04-05

  Online published: 2018-10-20

Abstract

In order to reveal the mechanism of stress-induced metabolism disorders of liver in poultry, this study explored the effect of glucocorticoid-dexamethasone (DEX) on the expression of fat and bile acid metabolism related genes in chicken embryo hepatocytes. Nineteen embryo age specific pathogen free (SPF) eggs were selected, and primary cultured (37℃, 5% CO2)chicken embryo hepatocytes were treated with 0 (control), 200, 500, and 1 000 nmol/L dexamethasone respectively for 24 h. The results showed as follows:compared with the control group, treated with the high dose (500 or 1 000 nmol/L) dexamethasone significantly reduced the mRNA relative expression levels of fat metabolism related genes, such as fatty acid transporter 1 (FATP-1), sterol regulatory element binding protein-1C (SREBP-1C), apolipoprotein B100 (APOB100) and liver X receptor (LXR) (P<0.05), and the mRNA relative expression level of bile acid uptake related gene Na+/taurocholate co-transporting polypeptide (NTCP) was also reduced (P<0.05); While the mRNA relative expression level of bile acid excretion related gene bile salt output pump (BSEP) was significantly increased (P<0.05); treated with the low dose (200 nmol/L) dexamethasone significantly increased the mRNA relative expression levels of bile acid synthesis related genes cholesterol7-alpha hydroxylase (CYP7A1) and farnesoid X receptor (FXR) (P<0.05), meanwhile, the SREBP-1C mRNA relative expression level was significantly decreased (P<0.05), and the BSEP mRNA relative expression level was significantly increased (P<0.05). In conclusion, high dose glucocorticoids can inhibit the synthesis and transport of fat and bile acid intake in chicken embryo hepatocytes, while low dose glucocorticoids can promote the synthesis and excretion of bile acids, some of these reactions have a dose-dependent response.

Cite this article

ZHOU Huajin, YANG Jiachang, ZHANG Hui, WANG Jiguang, HAO Yangyang, DU Peng, SONG Zhigang . Effects of Glucocorticoids on Expression of Lipid and Bile Acid Metabolism Related Genes in Chicken Embryo Hepatocytes[J]. Chinese Journal of Animal Nutrition, 2018 , 30(10) : 4262 -4268 . DOI: 10.3969/j.issn.1006-267x.2018.10.053

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