Effects of Dietary Hydrolyzed Wheat Gluten in Late Pregnancy on Reproductive Performance, Serum Biochemical Parameters and Amino Acid Contents, and Placental Tissue Nutrient Transporters Related Gene Expression of Sows

KOU Jiao, LIANG Haiwei, LIU Ning, DAI Zhaolai, LI Ju, PAN Junliang, ZHU Fangzhou, WU Guoyao, WU Zhenlong

doi:10.3969/j.issn.1006-267x.2018.11.013

2018 , Vol. 30 >Issue 11: 4369 - 4378

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.013

Molecular Nutrition

Effects of Dietary Hydrolyzed Wheat Gluten in Late Pregnancy on Reproductive Performance, Serum Biochemical Parameters and Amino Acid Contents, and Placental Tissue Nutrient Transporters Related Gene Expression of Sows

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1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
2. Henan Yinfa Animal Husbandry Co., Ltd., Zhengzhou 451100, China;
3. Zhengzhou Newwill Nutrition Technology Co., Ltd., Zhengzhou 450100, China;
4. Department of Animal Science, Texas A & M University, College Station 77843, USA

Received date: 2018-05-03

Online published: 2018-11-20

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Abstract

This experiment was conducted to investigate the effects of dietary hydrolyzed wheat gluten (HWG) in late pregnancy on reproductive performance, serum biochemical parameters and amino acid contents, and placental tissue nutrient transporters related gene expression of sows. Thirty-three gestation sows (3 to 4 parities) with similar expected date of childbirth were randomly allocated to 3 groups with 11 replicates per group and 1 sow per replicate. Sows in the control group were fed a basal diet, and the others in the experimental groups were fed the basal diets supplemented with 1% and 2% HWG during day 90 to 114 of pregnancy, respectively. All diets were isonitrogenous and isocaloric. The results showed as follows:1) compared with the control group, dietary 1% HWG supplementation significantly increased the average birth weight of all piglets born alive of piglets (P<0.05), dietary 2% HWG supplementation significantly increased the total litter weight at birth for all piglets born and total litter weight at birth for all live piglets (P<0.05). 2) Compared with the control group, dietary 1% and 2% HWG supplementation significantly increased the contents of asparagine, histidine and ornithine in serum of sows on day 107 of pregnancy, and contents of histidine, isoleucine, leucine, phenylalanine and tyrosine in serum of umbilical cord on day 107 of pregnancy (P<0.05), whereas, significantly decreased the contents of ammonia, glycine in serum of sows and umbilical cord serum ammonia content (P<0.05); dietary 1% HWG supplementation significantly increased the serum valine content of sows (P<0.05), dietary 2% HWG supplementation significantly increased the contents of isoleucine and threonine in serum of sows, and contents of arginine, glusate, glutamine, glycine, methionine, serine, threonine, tryptophan, valine, albumin, glucose and alkaline phosphatase activity in serum of umbilical cord (P<0.05). 3) Compared with the control group, dietary 1% HWG supplementation significantly increased the mRNA relative expressions of sodium-dependent neutral amino acid transporter (SNAT)2, excitatoryamino acid transporter 3 (EAAT3), L-type amino acid transporter 2 (LAT2) and glucose transporter (GLUT)2 in placental tissue (P<0.05), dietary 2% HWG supplementation significantly increased the mRNA relative expressions of SNAT1, SNAT2, EAAT3, LAT2, peptide transporter (PepT)1, PepT2, GLUT1, GLUT2 and GLUT3 in placental tissue (P<0.05). In conclusion, dietary HWG in late pregnancy can improve the placental tissue nutrient transporters related gene expression, promote the efficiency of transport efficiency of maternal nutrients and the growth and development of fetal pigs, improve the total litter weight at birth for all piglets born.

Key words： hydrolyzed wheat gluten; gestation sows; reproductive performance; serum biochemical parameters; amino acids; placenta

Cite this article

KOU Jiao, LIANG Haiwei, LIU Ning, DAI Zhaolai, LI Ju, PAN Junliang, ZHU Fangzhou, WU Guoyao, WU Zhenlong . Effects of Dietary Hydrolyzed Wheat Gluten in Late Pregnancy on Reproductive Performance, Serum Biochemical Parameters and Amino Acid Contents, and Placental Tissue Nutrient Transporters Related Gene Expression of Sows[J]. Chinese Journal of Animal Nutrition, 2018 , 30(11) : 4369 -4378 . DOI: 10.3969/j.issn.1006-267x.2018.11.013

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