The aim of this study was to investigate the effects of dietary different levels of folic acid and vitamin B₁₂ (VB₁₂) on growth performance, slaughter performance, fat deposition, very long chain fatty acid elongase 7 (ELOVL7) gene expression level and expression difference in different tissues of Wulong geese, and to analyze the correlation. A total of 420 Wulong geese at 5 weeks of age were randomly divided into 7 groups. Each group had 6 replicates and each replicate consisted of 5-male and 5-female geese. GroupsⅠto Ⅵ were experimental groups,and groupⅦ was a control group. The trial used a 2×3 factorial crossover design. The supplementation levels of folic acid were 0.25 and 2.00 mg/kg and the supplementation levels of VB₁₂ were 0.003 and 0.009,0.018 mg/kg. The experiment lasted for 4 weeks. The Real-time PCR was used to test the ELOVL7 gene expression level in the liver of Wulong geese in groups, and geese in the control group was selected to detect ELOVL7 gene expression in 11 tissues of heart, liver, kidney, abdominal fat, lung, gizzard, gland stomach, breast muscle, leg muscle, spleen and pancreas. The results showed as follows:1) the interaction of dietary different levels of folic acid and VB₁₆ had a significant effect on final weight of Wulong geese (P<0.05), the weight of group Ⅴ was the highest, and had no significant effect on average daily gain and feed to gain ratio (P>0.05). 2) The interaction of dietary different levels of folic acid and VB₁₆ had significant effects on the contents of triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) in serum (P<0.05). Dietary different levels of folic acid had significant effects on the contents of TG and LDL-C in serum (P<0.05), and dietary different levels of VB₁₂ had significant effects on the contents of glucose (GLU) and LDL-C in serum (P<0.05). 3) The interaction of dietary different levels of folic acid and VB₁₆ had a significant effect on the percentage of breast muscle (P<0.05), and had no significant effects on dressing percentage, percentage of half-eviscerated yield, percentage of eviscerated yield and percentage of leg muscle (P>0.05). The percentage of breast muscle in groups Ⅰ, Ⅴ was significantly higher than that in control group (P<0.05). 4) The interaction of dietary different levels of folic acid and VB₁₆ had significant effects on subcutaneous fat percentage, abdominal fat percentage, fat strip width, intramuscular fat (IMF) percentage in the breast muscle and IMF percentage in the leg muscle (P<0.05), and the subcutaneous fat percentage in group Ⅴ was significantly lower than that in other groups (P<0.05). 5) The interaction of dietary different levels of folic acid and VB₁₆ had a significant effect on ELOVL7 gene expression level in the liver (P<0.05); dietary different levels of folic acid or VB₁₆ could increase ELOVL7 gene expression level in the liver compared with the control group (P<0.05). 6) The ELOVL7 gene expression level in the liver had significantly negative correlation with dressing percentage (P<0.05), TG and LDL-C contents in serum (P<0.05), total cholesterol (TC) content in serum (P<0.01), subcutaneous fat percentage, abdominal fat percentage, IMF percentage in the leg muscle (P<0.05), and had significantly positive correlation with fat strip width (P<0.05). 7)The ELOVL7 gene expression level in the liver was the highest in abdominal fat percentage, and followed by the lung and pancreas, and that in the leg muscle and breast muscle was the lowest. In conclusion, 1) dietary different levels of folic acid and VB₁₂ significantly altered the expression of ELOVL7 gene, lipid metabolism biochemical indexes and organic nutrient composition of Wulong geese; 2) the 0.25 mg/kg folic acid and 0.009 mg/kg VB₁₂ (group Ⅴ) is the best combination.