Correlation Research between Furosine Content and Ruminal Degradation Characteristics and Intestinal Digestibility of Rumen Undegraded Protein in Distillers Dried Grains with Soluble

XU Hongjian, LI Xin, WANG Yujie, HAN Chunlei, ZHANG Yonggen

doi:10.3969/j.issn.1006-267x.2018.11.024

2018 , Vol. 30 >Issue 11: 4470 - 4481

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.024

Molecular Nutrition

Correlation Research between Furosine Content and Ruminal Degradation Characteristics and Intestinal Digestibility of Rumen Undegraded Protein in Distillers Dried Grains with Soluble

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College of Animal Science and Technology of Northeast Agricultural University, Harbin 150030, China

Received date: 2018-04-25

Online published: 2018-11-20

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Abstract

The aim of this experiment was to verify the correlation between the furosine content of Maillard reaction intermediates and the rumen degradation characteristic, intestinal digestibility of rumen undegraded protein of distillers dried grains with soluble (DDGS) with different degrees of heat processing, to establish regression equations, and to compare with the acid detergent insoluble protein as a sensitive index for heat treatment of feeds. In this study, high-performance liquid chromatography was used to determine furosine content, nylon bag method was used to determine the rumen degradation characteristics of dry matter and crude protein, and a modified three-step in vitro method was used to determine the intestinal digestibility of rumen undegraded protein in DDGS with different degrees of heat processing, to analyze correlation and establish regression equations. The results showed as follows:1) with increasing heating degree, the furosine and acid detergent insoluble protein contents in DDGS significantly increased (P<0.05), and the rumen degradation rate and intestinal digestibility of undegraded protein of dry matter and crude protein in DDGS significantly decreased (P<0.05). 2) Both furosine and acid detergent insoluble protein contents were significantly related to soluble fraction of dry matter and crude protein (r=-0.72 vs. -0.60; r=-0.60 vs. -0.51), undegradable fraction (r=0.96 vs. 0.84; r=0.96 vs. 0.85), effective degradation rate (r=-0.62 vs. -0.51; r=-0.72 vs. -0.61) and intestinal digestibility of rumen undegradable protein (r=-0.52 vs. -0.57), and the correlation was significant (P<0.05). The prediction equations could be established by the regression. 3) Furosine content had a higher correlation with ruminal degradation characteristics than acid detergent insoluble protein content. Both had similar correlations with the intestinal digestibility of rumen undegradable protein and total digestible protein content, but furosine and small intestine digestible protein content had a higher correlation(r=0.67). There is a correlation between furosine content and ruminal degradation characteristics and intestinal digestibility in DDGS with different heat processing, and a regression equation can be established. Furosine content is more suitable than acid detergent insoluble protein content as a key indicator to evaluate the degree of heat processing.

Key words： furosine; rumen degradable characteristics; intestinal digestibility; DDGS; heat processing

Cite this article

XU Hongjian, LI Xin, WANG Yujie, HAN Chunlei, ZHANG Yonggen . Correlation Research between Furosine Content and Ruminal Degradation Characteristics and Intestinal Digestibility of Rumen Undegraded Protein in Distillers Dried Grains with Soluble[J]. Chinese Journal of Animal Nutrition, 2018 , 30(11) : 4470 -4481 . DOI: 10.3969/j.issn.1006-267x.2018.11.024

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