Effects of Dietary n-3 Highly Unsaturated Fatty Acids Level on Growth Performance, Serum Biochemical Indices and Fatty Acid Composition in Muscle and Liver of Larger Cobia (Rachycentron canadum)

HUANG Qincheng, SHI Jun, DONG Xiaohui, TAN Beiping, CHI Shuyan, YNAG Qihui, ZHANG Shuang, LIU Hongyu, YANG Yuanzhi

doi:10.3969/j.issn.1006-267x.2018.11.026

2018 , Vol. 30 >Issue 11: 4490 - 4503

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.026

Molecular Nutrition

Effects of Dietary n-3 Highly Unsaturated Fatty Acids Level on Growth Performance, Serum Biochemical Indices and Fatty Acid Composition in Muscle and Liver of Larger Cobia (Rachycentron canadum)

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1. Laboratory of Aquatic Animal Nutrition and Feed, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;
2. China Key Laboratory of Aquatic, Livestock and Poultry Feed Science and Technology in South China, Ministry of Agriculture, Zhanjiang, Zhanjiang 524000, China

Received date: 2018-04-16

Online published: 2018-11-20

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Abstract

This experiment was carried out to determine the effects of dietary n-3 highly unsaturated fatty acids (n-3HUFA) level on growth performance, serum biochemical indices and fatty acid composition in muscle and liver of larger cobia (Rachycentron canadum), in order to determine the suitable dietary n-3HUFA level of larger cobia. Seven isonitrogenous and isoenergetic experimental diets were formulated with fish meal, casein and peeled soybean meal as main protein sources and different proportions of fish oil and corn oil as lipid sources, and cobias with the initial body weight of 70 g were fed 7 isonitrogenous and isoenergetic diets containing grade levels of n-3HUFA (0.49%, 0.73%, 0.98%, 1.41%, 1.51%, 2.06% and 2.83%) for 8 weeks. Fish in 3 cages (replicates) fed one kind of diet, and each cage cultured 30 fish. The results showed as follows:1) the weight gain rate (WGR) and specific growth rate (SGR) were increased firstly and then decreased with the dietary n-3HUFA level increasing, and those in 0.73% to 1.51% groups were significantly higher than those in 0.49% and 2.83% groups (P<0.05). The survival rate (SR) in 2.83% group was significantly lower than that in other groups (P<0.05). The body crude lipid content was increased firstly and then decreased with the dietary n-3HUFA level increasing, and that in 0.98% and 1.41% groups was significantly higher than that in 2.06% and 2.83% groups (P<0.05). 2) Serum alanine transaminase (ALT) activity in 0.49% group was significantly higher than that in 2.06% group (P<0.05). Serum triglyceride (TG) content in 0.49% group was significantly higher than that in other groups (P<0.05). Serum total cholesterol (CHOL) content was increased firstly and then decreased with the dietary n-3HUFA level increasing, and the highest value was found in 0.73% group, which significantly higher than 0.49%, 2.06% and 2.83% groups (P<0.05). Serum high-density lipoprotein (HDL) content in 0.98% group was significantly higher than that in other groups (P<0.05). Serum low-density lipoprotein (LDL) content in 0.98% to 2.83% groups was significantly higher than that in 0.49% and 0.73% groups (P<0.05). 3) The highest C14:0 and C21:0 contents in muscle and liver were found in 2.83% group, and C16:0 content and the total amount of saturated fatty acids (∑SFA) were not significantly influenced by dietary n-3HUFA level (P>0.05). C18:1n-9, C18:2n-6 contents and the total amount of monounsaturated fatty acids (∑MUFA) all showed a decreased trend with the dietary n-3HUFA level increasing. With the dietary n-3HUFA level increasing, the total amount of n-6 polyunsaturated fatty acids (∑n-6PUFA) increased firstly and then kept stable, and the contents of C20:5n-3 (eicosapentaenoic acid), C22:5n-3, C22:6n-3 (docosahexaenoic acid), the total amount of n-3 polyunsaturated fatty acids (∑n-3PUFA) and the total amount of n-3HUFA (∑n-3HUFA) all showed a gradually rising tendency (P<0.05). In conclusion, dietary n-3HUFA positively improves growth performance of lager cobia and decreases the body lipid deposition, enhances lipid metabolization, and affects the fatty acid composition in body and liver. With the SGR as evaluation index, the suitable dietary n-3HUFA level of larger cobia is 0.95% by broken-line model.

Key words： larger cobia (Rachycentron canadum); n-3HUFA; growth; serum biochemical indices; fatty acid composition

Cite this article

HUANG Qincheng, SHI Jun, DONG Xiaohui, TAN Beiping, CHI Shuyan, YNAG Qihui, ZHANG Shuang, LIU Hongyu, YANG Yuanzhi . Effects of Dietary n-3 Highly Unsaturated Fatty Acids Level on Growth Performance, Serum Biochemical Indices and Fatty Acid Composition in Muscle and Liver of Larger Cobia (Rachycentron canadum)[J]. Chinese Journal of Animal Nutrition, 2018 , 30(11) : 4490 -4503 . DOI: 10.3969/j.issn.1006-267x.2018.11.026

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