Molecular Nutrition

Effects of Organic Acids (Salt) Supplementation in Low Fish Meal Diet on Growth Performance, Digestive Enzyme Activities and Nutrient Apparent Digestibility of Litopenaeus vannamei

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  • 1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;
    2. Key Laboratory of Freshwater Fishery Cermplasm Resources, Ministry of Aquaculture, Shanghai 201306, China;
    3. Shanghai University Knowledge Service Platform, Aquatic Animal Breeding Center, Shanghai Ocean University, Shanghai 201306, China

Received date: 2018-04-28

  Online published: 2018-11-20

Abstract

This study was conducted to evaluate the effects of organic acid (salt) supplementation in low fish meal diet on growth performance, digestive enzyme activities and nutrient apparent digestibility of Litopenaeus vannamei. A total of 1 080 Litopenaeus vannamei with an initial body weight of (4.60±0.05) g were randomly divided into 9 groups with 4 replicates per group and 30 shrimps per replicate. Shrimps in the positive control group (PC group) were fed a basal diet which contained 18% fish meal; shrimps in two negative control groups were fed the experimental diets which used meat and bone meal (MBM group) and soybean meal (SM group) replaced 1/3 fish meal, respectively; shrimps in experimental groups were fed the experimental diets of negative control groups supplemented with 0.2% citric acid (MBM-CA and SM-CA groups), malic acid (MBM-MA and SM-MA groups) and sodium butyrate (MBM-SB and SM-SB groups), respectively. The experiment lasted for 9 weeks. The results showed that compared with the PC group, the weight gain rate and apparent digestibility of crude protein and crude lipid in MBM and SM groups were significantly decreased (P<0.05), and the feed conversion ratio was increased (P<0.05). The meat and bone meal diet and soybean meal diet supplemented with citric acid and sodium butyrate could significantly increase the weight gain rate, protein retention and lipid retention and dry matter apparent digestibility of Litopenaeus vannamei (P<0.05), and significantly decrease the feed conversion ratio (P<0.05); the meat and bone meal diet and soybean meal diet supplemented with malic acid did not significantly affect the weight gain rate, feed conversion ratio, protein retention, lipid retention and apparent digestibility of dry matter, crude protein, crude lipid of Litopenaeus vannamei (P>0.05). There were no significant differences on the activities of protease and amylase in hepatopancreas of Litopenaeus vannamei (P>0.05). In summary, the supplementation of 0.2% citric acid or sodium butyrate in low fish meal diet (12% fish meal) can significantly promote the growth performance and feed efficiency of Litopenaeus vannamei, but the supplementation of 0.2% malic acid has no significant effect on the growth performance and feed efficiency.

Cite this article

HUAN Dianyu, LI Xiaoqin, YAO Wenxiang, YANG Hang, LIANG Gaoyang, LENG Xiangjun . Effects of Organic Acids (Salt) Supplementation in Low Fish Meal Diet on Growth Performance, Digestive Enzyme Activities and Nutrient Apparent Digestibility of Litopenaeus vannamei[J]. Chinese Journal of Animal Nutrition, 2018 , 30(11) : 4526 -4537 . DOI: 10.3969/j.issn.1006-267x.2018.11.029

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