Effects of Cysteamine Hydrochloride on Fur Quality, Serum Biochemical and Hormone Parameters and Liver Related Gene Expression of Male Minks during Winter Fur-Growing Period

SUN Weili, FAN Yanyan, ZHANG Ting, WANG Zhuo, LI Hengwei, LI Guangyu

doi:10.3969/j.issn.1006-267x.2018.11.032

2018 , Vol. 30 >Issue 11: 4559 - 4568

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.032

Molecular Nutrition

Effects of Cysteamine Hydrochloride on Fur Quality, Serum Biochemical and Hormone Parameters and Liver Related Gene Expression of Male Minks during Winter Fur-Growing Period

Expand

1. Institute of Special Animal and Plant Sciences, Chinese Academy of Agriculture Sciences, Jilin Provincial Key Laboratory for Molecular Biology of Special Economic Animals, Changchun 130112, China;
2. Muyuan Food Technology Co., Ltd., Luoyang 420106, China;
3. Shandong Yakang Detection Technology Co., Ltd., Weifang 261101, China

Received date: 2018-04-19

Online published: 2018-11-20

Fold

Abstract

This experiment was conducted to investigate the effects of dietary cysteamine hydrochloride (CSH) on fur quality, serum biochemical and hormone parameters and liver related gene expression of male minks during winter fur-growing period. Fifty six 160-day-old male minks with similar body weight[(2.13±0.10) kg] were randomly divided into 7 groups with 8 replicates per group and 1 mink per replicate. Minks were fed the basal diets supplemented with 0 (group Ⅰ, control group) 60 (group Ⅱ), 90 (group Ⅲ), 120 (group Ⅳ), 60 (group Ⅴ), 90 (group Ⅵ) and 120 mg/kg (group Ⅶ) CSH, respectively; the supplemental way of groups Ⅱ, Ⅲ and Ⅳ was continuous supplementation, and the supplemental way of groups Ⅴ, Ⅵ and Ⅶ was interval supplementation (1 week continuous, 1 weeks interval). The pre-experimental period lasted for 7 days, and the experimental period lasted for 51 days. The results showed as follows:1) the skin length, aciculum length and villus length of minks in groups Ⅵ and Ⅶ were significantly higher than those in the control group (P<0.05 or P<0.01). 2) The serum urea nitrogen content in groups Ⅱ, Ⅲ, Ⅴ, Ⅵ and Ⅶ was significantly lower than that in the control group (P<0.05 or P<0.01), and the serum triglyceride content in groups Ⅱ, Ⅲ, Ⅴ, Ⅵ and Ⅶ was significantly lower than that in the control group (P<0.05 or P<0.01). 3) The serum growth hormone content in group Ⅵ was significantly higher than that in the control group and group Ⅳ (P<0.05), and the serum somatostatin content in groups Ⅱ, Ⅲ and Ⅵ was significantly lower than that in the control group and group Ⅳ (P<0.05), the serum growth hormone receptor content in groups Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ and Ⅶ was significantly higher than that in the control group (P<0.05 or P<0.01), the serum insulin-like growth factor-Ⅰ content in groups Ⅱ, Ⅲ, Ⅴ, Ⅵ and Ⅶ was significantly higher than that in the control Ⅳ (P<0.05 or P<0.01). 4) The liver insulin-like growth factor-Ⅰ gene expression level in group Ⅵ was significantly higher than that in the control group and groups Ⅱ, Ⅳ, Ⅴ (P<0.05), the liver insulin-like growth factor-Ⅰ receptor gene expression level in group Ⅵ was significantly higher than that in groups Ⅱ, Ⅳ, Ⅴ (P<0.05), the liver growth hormone receptor gene expression level in groups Ⅱ, Ⅲ, Ⅳ, Ⅴ, Ⅵ and Ⅶ was significantly higher than that in the control group (P<0.05 or P<0.01). In conclusion, in this experimental condition, the optimal CSH supplemental level for diets of male minks during winter fur-growing period is 90 mg/kg, and the optimal supplemental way was interval supplementation.

Key words： cysteamine hydrochloride; minks; fur quality; serum parameters; gene expression

Cite this article

SUN Weili, FAN Yanyan, ZHANG Ting, WANG Zhuo, LI Hengwei, LI Guangyu . Effects of Cysteamine Hydrochloride on Fur Quality, Serum Biochemical and Hormone Parameters and Liver Related Gene Expression of Male Minks during Winter Fur-Growing Period[J]. Chinese Journal of Animal Nutrition, 2018 , 30(11) : 4559 -4568 . DOI: 10.3969/j.issn.1006-267x.2018.11.032

References

[1] SZABO S,RCICHIN S.Somatostatin in rat tissues is depleted by cysteam.ine administration[J].Endocrinology,1981,109(2):2255-2257.

[2] PALKOVITS M,BROWNSTEIN M J,EIDEN L E,et al.Selective depletion of somatostatin in rat brain by cysteamine[J].Brain Research,1982,240(1):178-180.

[3] MILLARD W J,SAGAR S M,LAUDIS D M,et al.Cysteamine:a potent and specific depletor of pituitary prolactin[J].Science,1982,217(4558):452-454.

[4] DILIBERTO E J,Jr,DISTEFANO V,SMITH J C.Mechanism and kinetics of the inhibition of dopamine-β-hydroxylase by 2-mercaptoethylguanidine[J].Biochemical Pharmacology,1973,22(23):2961-2972.

[5] SAGAR S M,LANDRY D,MILLARD W J,et al.Depletion of somatostatin-like immunoreactivity in the rat central nervous system by cysteamine[J].Journal of Neuroscience,1982,2(2):225-231.

[6] SAGAR S M,MARTIN J B.The effect of cysteamine on immunoreactive somatostatin in the rabbit retina[J].Neuroscience Letters,1982,34(3):265-269.

[7] PATEL Y C,PIERZCHALA I,AMHERDT M,et al.Effects of cysteamine and antibody to somatostatin on islet cell function in vitro.Evidence that intracellular somatostatin deficiency augments insulin and glucagon secretion[J].The Journal of Clinical Investigation,1985,75(4):1249-1255.

[8] POULSEN S S,KIRKEGAARD P,OLSEN P S,et al.Role of delayed gastric emptying in the pathogenesis of cysteamine-induced duodenal ulcer in the rat[J].Scandinavian Journal of Gastroenterology,1982,17(3):325-330.

[9] MCLEOD K R,HARMON D L,SCHILLO K K,et al.Effects of cysteamine on pulsatile growth hormone release and plasma insulin concentrations in sheep[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,1995,112(3):523-533.

[10] 王忠艳.半胱胺在银狐育成期、生长期和准备配种期饲料中的应用[D].博士学位论文.哈尔滨:东北林业大学,2006.

[11] 白世平,袁缨,权志中,等.日粮中添加半胱胺对雄性水貂的生产性能、血液激素水平的影响[J].经济动物学报,2006,10(3):133-136.

[12] 黄卉.饲料中添加半胱胺对貉生产性能的影响[D].硕士学位论文.哈尔滨:东北林业大学,2015.

[13] 徐银学,李建武,龚卫忠,等.CS87与添加剂对海狸鼠生长及有关激素水平的影响[J].畜牧与兽医,2001,33(5):9-11.

[14] NRC.Nutrient requirements of mink and foxes[S].2nd ed.Washington,D.C.:National Academy Press,1982.

[15] 张海华.日粮蛋白质和蛋氨酸水平对水貂生产性能及毛皮发育的影响[D].博士学位论文.北京:中国农业科学院,2011.

[16] 黄继卓,任道平,胡英爽.半胱胺和大豆黄酮对东北细毛羊生长性能和羊毛品质的影响[J].黑龙江畜牧兽医,2009(6):54-55.

[17] 徐军,刘晳洁.日粮中添加半胱胺对辽宁绒山羊产绒性能的影响[J].中国畜牧兽医,2010,37(7):210-212.

[18] 赵国先,宋智娟,张晓云,等.毛皮质量的营养调控[J].中国饲料,2005(13):5-6,9.

[19] 王建红,刁其玉,许先查,等.赖氨酸、蛋氨酸和苏氨酸对犊牛生长性能和血清生化指标的影响[J].动物营养学报,2011,23(2):226-233.

[20] 钟诚,方国玺,李凤双.青山羊血清含氮生化指标与蛋白质营养代谢关系的研究[J].山东农业大学学报,1986,17(1):83-88.

[21] 樊燕燕,孙伟丽,王卓,等.半胱胺对冬毛期雄性水貂生产性能、营养物质消化率及氮代谢的影响[J].动物营养学报,2016,28(10):3337-3345.

[22] 陈会敏,徐安莉,徐建民,等.牛蒡子提取物对高脂血症大鼠血脂及脂肪肝形成的影响[J].吉林中医药,2012,32(6):615-617.

[23] TANNENBAUM G S,LING N.The interrelationship of growth hormone(GH) releasing factor and somatostatin in generation of the ultradian rhythm of GH secretion[J].Endocrinology,1985,115(5):1952-1957.

[24] GAUSE I,EDÉN S.Induction of growth hormone (GH) receptors in adipocytes of hypophysectomized rats by GH[J].Endocrinology,1986,118(1):119-124.

[25] MAITER D,UNDERWOOD L E,MAES M,et al.Acute down-regulation of the somatogenic receptors in rat liver by a single injection of growth hormone[J].Endocrinology,1988,122(4):1291-1296.

[26] 赵宗胜,王根林,李大全,等.IGF-Ⅰ和EGF对绵羊毛囊体外培养的影响[J].南京农业大学学报,2006,29(2):138-141.

[27] LI Y,LIU X C,ZHANG Y,et al.Effects of cysteamine on mRNA levels of growth hormone and its receptors and growth in orange-spotted grouper (Epinephelus coioides)[J].Fish Physiology and Biochemistry,2013,39(3):605-613.

[28] 马细兰,张勇,刘晓春,等.半胱胺盐酸盐(CSH)对尼罗罗非鱼(Oreochromis niloticus)生长及生长轴相关基因表达的影响[J].海洋与湖沼,2010,41(2):240-245.

[29] 任道平,耿忠诚,刘胜军,等.半胱胺和大豆黄酮对东北细毛羊部分组织IGF-ⅠmRNA表达量的影响[J].动物营养学报,2009,21(6):967-973.

[30] VALENTINIS B,ROMANO G,PERUZZI F,et al.Growth and differentiation signals by the insulin-like growth factor 1 receptor in hemopoietic cells are mediated through different pathways[J].Journal of Biological Chemistry,1999,274(18):12423-12430.

[31] IOZZO R V,BURASCHI S,GENUA M,et al.Decorin antagonizes IGF receptor I (IGF-ⅠR) function by interfering with IGF-ⅠR activity and attenuating downstream signaling[J].Journal of Biological Chemistry,2011,286(40):34712-34721.

[32] SARFSTEIN R,PASMANIK-CHOR M,YEHESKEL A,et al.2012.Insulin-like growth factor-Ⅰ receptor (IGF-ⅠR) translocates to nucleus and autoregulates IGF-ⅠR gene expression in breast cancer cells[J].Journal of Biological Chemistry,2012,287(4):2766-2776.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References