Effects of Lactobacillus reuteri LR1 on Serum Biochemical Indexes and mRNA Expressions of Intestinal Nutrient Transporters of Weaned Piglets

YANG Guangda, WANG Li, YI Hongbo, WANG Zhilin, YANG Xuefen, GAO Kaiguo, WEN Xiaolu, JIANG Zongyong

doi:10.3969/j.issn.1006-267x.2018.11.035

2018 , Vol. 30 >Issue 11: 4589 - 4600

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.035

Molecular Nutrition

Effects of Lactobacillus reuteri LR1 on Serum Biochemical Indexes and mRNA Expressions of Intestinal Nutrient Transporters of Weaned Piglets

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1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
2. State Key Laboratory of Livestock and Poultry Breeding, Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South China, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2018-04-12

Online published: 2018-11-20

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Abstract

This experiment was conducted to investigate the effects of Lactobacillus reuteri (L. reuteri) LR1 on serum biochemical indexes and mRNA expressions of intestinal nutrient transporters of weaned piglets. A total of 144 weaned piglets (Duroc×Landrace×Yorkshire, 21 days of age) with an initial body weight of (6.49±0.01) kg were randomly allocated to 3 groups consisting of 8 replicates with 6 piglets each replicate. Piglets in control group were fed a basal diet, those in antibiotics group were fed the basal diet+100 mg/kg olaquindox+75 mg/kg aureomycin, and those in L. reuteri group were fed the basal diet+5×10¹⁰ CFU/kg L. reuteri LR1.The experiment lasted for 14 days. The results showed as follows:1) compared with the control group, antibiotics supplementation significantly increased serum glucose (GLU) content (P<0.05), and significantly decreased urea nitrogen (UN) content (P<0.05). 2) Compared with the control group, L. reuteri LR1 supplementation significantly increased the mRNA expressions of motilin (MLN) in duodenum and cholecystokinin (CCK) in jejunum (P<0.05); antibiotics supplementation significantly increased the mRNA expression of MLN in duodenum (P<0.05). 3) Compared with the control group, L. reuteri LR1 supplementation significantly increased the mRNA expressions of Na⁺ dependent glutamine transporter 2 (ASCT2), cationic amino acid transporter 1 (CAT1), peptide transporter 1 (PepT1) in duodenum, neutral and basic amino acid transport protein (rBAT), y⁺L amino acid transporter 1 (y⁺LAT1) in jejunum and y⁺LAT1 in ileum (P<0.05); antibiotics supplementation significantly increased the mRNA expressions of y⁺LAT1, CAT1, PepT1 in jejunum and mammalian target of rapamycin (mTOR) in jejunum and ileum (P<0.05). 4) Compared with the control group, L. reuteri LR1 supplementation significantly increased the mRNA expressions of intestinal fatty acid binding protein (I-FABP), fatty acid binding protein 3 (FABP3), acetyl CoA carboxylase α (ACCα), fatty acid synthase (FASN) in duodenum and FABP3 in jejunum and peroxisome proliferator-activated receptor γ (PPARγ) in duodenum, jejunum and ileum (P<0.05); antibiotics supplementation significantly increased the mRNA expression of ACCα in jejunum (P<0.05). 5) Compared with control group, L. reuteri LR1 supplementation significantly increased the mRNA expression of sodium-dependent glucose transporter 1 (SGLT1) in jejunum and ileum (P<0.05); antibiotics supplementation significantly increased the mRNA expressions of SGLT1 and sodium-dependent glucose transporter 3 (SGLT3) in duodenum (P<0.05). In conclusion, diet supplemented with 5×10¹⁰ CFU/kg L. reuteri LR1 has favorable promoting effects on transportation of intestinal nutrients in weaned piglets, characterized by promoting physical and chemical digestion, facilitating absorption and transportation of peptides, amino acids and fatty acid, enhancing protein and fatty acid synthesis in intestine of weaned piglets. Hence, L. reuteri LR1 has great potential for the replacement of in-feed antibiotics and can be used for development of antibiotics substitution of pig feeds.

Key words： Lactobacillus reuteri LR1; antibiotic; weaned piglets; nutrients; transporters

Cite this article

YANG Guangda, WANG Li, YI Hongbo, WANG Zhilin, YANG Xuefen, GAO Kaiguo, WEN Xiaolu, JIANG Zongyong . Effects of Lactobacillus reuteri LR1 on Serum Biochemical Indexes and mRNA Expressions of Intestinal Nutrient Transporters of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2018 , 30(11) : 4589 -4600 . DOI: 10.3969/j.issn.1006-267x.2018.11.035

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