Effects of Adding High Doses of Tea Polyphenols into Diets on Performance, Egg Quality and Lipid Metabolism of Laying Hens during Later Laying Period

HE Junjin, WANG Jianping, DING Xuemei, ZENG Qiufeng, BAI Shiping, ZHANG Keying

doi:10.3969/j.issn.1006-267x.2018.11.036

2018 , Vol. 30 >Issue 11: 4601 - 4610

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.036

Molecular Nutrition

Effects of Adding High Doses of Tea Polyphenols into Diets on Performance, Egg Quality and Lipid Metabolism of Laying Hens during Later Laying Period

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Key Laboratory for Animal Disease-Resistance Nutrition and Feed of Ministry, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2018-04-17

Online published: 2018-11-20

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Abstract

This experiment was conducted to study the effects of adding high doses of tea polyphenols (TP) into diets on performance, egg quality and serum lipometabolism parameters and hepatic lipometabolism-related gene expression of laying hens during later laying period. A total of 480 Lohmann laying hens (65-week-old) were randomly allotted into 4 groups with 8 replicates per group and 15 hens per replicate. The laying hens in control group were fed a basal diet, and those in experimental groups were fed the basal diets supplemented with 666, 1 333 and 2 666 mg/kg TP (its purity reached 63.58%, and the content of catechin in it was 40.20%), respectively. There was a pretrial period of 2 weeks followed by an experimental period of 9 weeks. The results showed as follows:1) compared with the control group, adding 2 666 mg/kg TP significantly decreased the laying rate of week 1 to 3, 4 to 6 and 1 to 9 (P<0.05), significantly decreased the average daily feed intake of week 1 to 3, 4 to 6, 7 to 9 and 1 to 9 (P<0.05), and significantly increased the feed/egg of week 1 to 3 and 4 to 6 (P<0.05); adding 1 333 and 2 666 mg/kg TP significantly decreased the average egg weight of week 1 to 3, 4 to 6 and 1 to 9 (P<0.05). 2) Compared with the control group, adding 2 666 mg/kg TP significantly increased the Haugh unit of week 2 (P<0.05), and significantly decreased the eggshell ratio of week 2 (P<0.05); adding 1 333 mg/kg TP significantly increased the Haugh unit of week 4 (P<0.05), and significantly decreased the eggshell ratio of week 2 (P<0.05); adding 666, 1 333 and 2 666 mg/kg TP significantly decreased egg yolk cholesterol content of week 9 (P<0.05); adding 666, 1 333 and 2 666 mg/kg TP did not significantly affect the eggshell strength, egg yolk color, egg yolk ratio, albumen ratio and egg yolk fatty acid composition (P>0.05). 3) Compared with the control group, adding 666, 1 333 and 2 666 mg/kg TP significantly decreased the serum total cholesterol (TC) and low density lipoprotein cholesterin (LDL-C) contents (P<0.05); adding 1 333 and 2 666 mg/kg TP significantly decreased the serum triglyceride (TG) content (P<0.05). 4) Compared with the control group, adding 666, 1 333 and 2 666 mg/kg TP significantly increased the mRNA relative expression level of AMP-activated protein kinase (AMPK) in liver (P<0.05). In conclusion, adding high doses (1 333 and 2 666 mg/kg) of TP into diets cause the decrease of performance of laying hens during later laying period, but can increase the Haugh unit of egg, decrease the serum TC and LDL-C contents and egg yolk cholesterol content, and up-regulate the expression of AMPK gene in liver.

Key words： tea polyphenols; laying hens; performance; egg quality; lipid metabolism

Cite this article

HE Junjin, WANG Jianping, DING Xuemei, ZENG Qiufeng, BAI Shiping, ZHANG Keying . Effects of Adding High Doses of Tea Polyphenols into Diets on Performance, Egg Quality and Lipid Metabolism of Laying Hens during Later Laying Period[J]. Chinese Journal of Animal Nutrition, 2018 , 30(11) : 4601 -4610 . DOI: 10.3969/j.issn.1006-267x.2018.11.036

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