Study on Correlation of Molecular Structure of Carbohydrate with Its Nutritional Value and Ruminal Degradation Characteristics in Different Heat Treated Okara

LI Xin, XU Hongjian, HUO Pengju, WANG Yujie, LIU Xin, YAO Enyue, ZHANG Yonggen, SUN Manji

doi:10.3969/j.issn.1006-267x.2018.11.046

2018 , Vol. 30 >Issue 11: 4683 - 4694

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.046

Molecular Nutrition

Study on Correlation of Molecular Structure of Carbohydrate with Its Nutritional Value and Ruminal Degradation Characteristics in Different Heat Treated Okara

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College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China

Received date: 2018-04-13

Online published: 2018-11-20

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Abstract

The purpose of this experiment is to explore the effect of different heat treated on the molecular structure of carbohydrates in okara and its relationship with nutritional value and ruminal degradation characteristics. The nutrient value of different heat treated okara was evaluated by conventional chemical analysis combined with Cornell net carbohydrate-protein system (CNCPS) and nylon bag technology, changes of the molecular structure of carbohydrates in okara was analyzed by Fourier transform infrared spectroscopy (FTIR) technique, then explore the correlation between the molecular structure of carbohydrates and nutritional value and ruminal degradation characteristics. The results showed as follows:1) heat treatment reduced the nutritional value of okara and the rumen degradability of neutral detergent fibers (NDF). 2) Different heat treatment had significant effect on the peak area of structural carbohydrates (STCHO), fiber composites (CELC), total carbohydrates (CHO) and the corresponding peak heights in the three peak areas in the molecular structure of carbohydrates in okara (P<0.05). 3) The peak area ratio of STCHO to CHO and the peak to height ratio of total carbohydrates can effectively estimate the nutritional value of different heat treated okara and the content of NDF rumen degradation parameters. In summary, different heat treatment have effects on the molecular structure, nutritional value and ruminal degradability of NDF in okara, and there is a correlation between the molecular structure of carbohydrates in okara and their nutritional value along with ruminal degradability in NDF. It is proved that the spectral information of okara obtained by FTIR spectroscopy can directly reflect the degree of thermal damage.

Key words： heat treated; okara; FTIR technique; carbohydrate molecular structure; correlation

Cite this article

LI Xin, XU Hongjian, HUO Pengju, WANG Yujie, LIU Xin, YAO Enyue, ZHANG Yonggen, SUN Manji . Study on Correlation of Molecular Structure of Carbohydrate with Its Nutritional Value and Ruminal Degradation Characteristics in Different Heat Treated Okara[J]. Chinese Journal of Animal Nutrition, 2018 , 30(11) : 4683 -4694 . DOI: 10.3969/j.issn.1006-267x.2018.11.046

References

[1] 祝义伟,龙勃,龙勇,等.豆渣中营养成分的检测及其含量声称[J].食品研究与开发,2017,38(8):117-120.

[2] 李岩,孟庆祥,陈万宝.应用CNCPS方法和体外产气法研究豆腐渣饲料的营养价值[J].中国畜牧兽医,2017,44(5):1355-1362.

[3] 张学燕,崔占鸿,孙璐,等.利用体外产气法及CNCPS体系评定豆渣、菜籽粕的营养价值[J].青海畜牧兽医杂志,2017,47(3):18-22.

[4] 张永根,薛世崇,李洋,等.固态发酵豆渣作生产反刍动物饲料工艺条件的研究[J].东北农业大学学报,2016,47(5):76-82.

[5] 潘树国.废豆渣生产生物蛋白饲料可行性研究[J].现代畜牧科技,2017(10):8-9.

[6] 张文佳.产朊假丝酵母和白地霉混合固态发酵豆渣生产反刍动物饲料的研究[D].硕士学位论文.哈尔滨:东北农业大学,2015.

[7] GONZÁLEZ-VEGA J C,KIM B G,HTOO J K,等.热处理豆粕饲喂生长猪的氨基酸消化率[J].中国饲料,2015(24):32-37.

[8] XIN H S,DING X,ZHANG L Y,et al.Investigation of the spectroscopic information on functional groups related to carbohydrates in different morphological fractions of corn stover and their relationship to nutrient supply and biodegradation characteristics[J].Journal of Agricultural and Food Chemistry,2017,65(20):4035-4043.

[9] LIU N,YU P Q.Molecular clustering,interrelationships and carbohydrate conformation in hull and seeds among barley cultivars[J].Journal of Cereal Science,2011,53(3):379-383.

[10] GHOLIZADEH H,NASERIAN A A,XIN H S,et al.Detecting carbohydrate molecular structural makeup in different types of cereal grains and different cultivars within each type of grain grown in semi-arid area using FTIR spectroscopy with uni-and multi-variate molecular spectral analyses[J].Animal Feed Science and Technology,2014,194:136-144.

[11] PRATES L L,REFAT B,LEI Y G,et al.Relationship of carbohydrates and lignin molecular structure spectral profiles to nutrient profile in newly developed oats cultivars and barley grain[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2018,188:495-506.

[12] JI C Y,ZHANG X W,YAN X G,et al.Bio-functions and molecular carbohydrate structure association study in forage with different source origins revealed using non-destructive vibrational molecular spectroscopy techniques[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2017,183:260-266.

[13] 张平安,张建威,宋连军,等.豆渣微波热风联合干燥特性研究[J].南方农业学报,2011,42(5):528-530.

[14] 李波,张延生,李瑞峰,等.不同干燥方法对豆腐渣功能性质的影响[J].河南科技学院学报(自然科学版),2008,36(3):64-66.

[15] NRC.Nutrient requirements of dairy cattle[M].7th ed.Washington,D.C.:National Academy Press,2001.

[16] 杨胜.饲料分析及饲料质量检测技术[M].北京:北京农业大学出版社,1999.

[17] 张旭,蒋桂韬,王向荣,等.酶法测定谷物副产品中淀粉含量[J].广东饲料,2013,22(10):33-35.

[18] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.

[19] SNIFFEN C J,O'CONNOR J D,VAN SOEST P J,et al.A net carbohydrate and protein system for evaluating cattle diets:Ⅱ.Carbohydrate and protein availability[J].Journal of Animal Science,1992,70(11):3562-3577.

[20] PENG Q H,KHAN N A,WANG Z S,et al.Moist and dry heating-induced changes in protein molecular structure,protein subfractions,and nutrient profiles in camelina seeds[J].Journal of Dairy Science,2014,97(1):446-457.

[21] ØRSKOV E R,MCDONALD I.The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage[J].The Journal of Agricultural Science,1979,92(2):499-503.

[22] 高红,郝小燕,张广宁,等.应用体外产气法和尼龙袋法评价几种粮食加工副产物的营养价值[J].中国饲料,2017(7):14-19.

[23] SAMADI,THEODORIDOU K,YU P Q.Detect the sensitivity and response of protein molecular structure of whole canola seed (yellow and brown) to different heat processing methods and relation to protein utilization and availability using ATR-FT/IR molecular spectroscopy with chemometrics[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2013,105:304-313.

[24] FALES S L.温度对高羊茅的纤维含量、组成及体外消化动力学的影响[J].草原与业,1988(3):46-50.

[25] NUEZ-ORTÍN W G,YU P.Estimation of ruminal and intestinal digestion profiles,hourly effective degradation ratio and potential N to energy synchronization of co-products from bioethanol processing[J].Journal of the Science of Food and Agriculture,2010,90(12):2058-2067.

[26] MCKINNON J J,OLUBOBOKUN J A,MUSTAFA A,et al.Influence of dry heat treatment of canola meal on site and extent of nutrient disappearance in ruminants[J].Animal Feed Science and Technology,1995,56(3/4):243-252.

[27] XIN H S,YU P Q.Using ATR-FT/IR to detect carbohydrate-related molecular structure features of carinata meal and their in situ residues of ruminal fermentation in comparison with canola meal[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2013,114:599-606.

[28] YU P Q,DAMIRAN D,AZARFAR A,et al.Detecting molecular features of spectra mainly associated with structural and non-structural carbohydrates in co-products from bioethanol production using DRIFT with uni-and multivariate molecular spectral analyses[J].International Journal of Molecular Sciences,2011,12(3):1921-1935.

[29] XIN H S,FALK K C,YU P Q.Studies on Brassica carinata seed.2.Carbohydrate molecular structure in relation to carbohydrate chemical profile,energy values,and biodegradation characteristics[J].Journal of Agricultural and Food Chemistry,2013,61(42):10127-10134.

[30] 李欣新.双低菜粕和豆粕分子结构与营养特性和奶牛生产性能的关系[D].博士学位论文.哈尔滨:东北农业大学,2016.

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