The present study evaluated the effects of zinc supplemental level on tissue and organ zinc deposition and tibia indexes by adding different levels of zinc into the basal diet of Jinghong No.1 layer hens during laying peak period. Five hundred and forty 20-week-old healthy Jinghong No.1 layer hens were randomly allotted to 6 groups with 6 replicates per group and 15 layer hens per replicate. Birds in control group was fed a basal diet (contained 25 mg/kg zinc), and birds in trial groups were fed experimental diets which added 25, 50, 75, 100 and 125 mg/kg zinc (using sulfate zinc monohydrate as zinc source) on the basis of the basal diet. The pre-feeding period was 2 weeks and the experimental period was 24 weeks. The results showed as follows:1) adding different levels zinc into diets significantly or extremely significantly enhanced the serum zinc content of Jinghong No.1 layer hens at the end of 33 weeks of age (P<0.05 or P<0.01), but it did not significantly affect the serum zinc content at the end of 46 weeks of age (P>0.05). 2) Adding different levels zinc into diets extremely significantly affected the liver zinc content of Jinghong No.1 layer hens at the end of 46 weeks of age (P<0.01), and the liver zinc content was enhanced as the zinc supplemental level increasing, but did not significantly affect the muscle and pancreas zinc content (P>0.05). 3) Adding different levels zinc into diets did not significantly affect the tibia indxes including tibia weight, fat free weight, tibia ash weight, tibia zinc content and tibia ash zinc content of Jinghong No.1 layer hens at the end of 46 weeks of age (P>0.05). 4) Adding different levels zinc into diets extremely significantly affected the feces zinc content and zinc apparent availability of Jinghong No.1 layer hens at the end of 46 weeks of age (P<0.01), the feces zinc content was enhanced and the zinc apparent availability was reduced as the zinc supplemental level increasing. Comprehensive consideration, the optimal supplemental level of zinc is 75 mg/kg using sulfate zinc monohydrate as a zinc source in the basal diet containing 25 mg zinc of Jinghong No.1 layer hens during laying peak period.