Effects of Dietary &beta;-Glucan on Growth Performance and Intestinal Microflora of Weaned Piglets

CHEN Qingju, LIU Jinyan, LU Changwen, MA Yajun, TANG Zhiru

doi:10.3969/j.issn.1006-267x.2018.11.049

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 11: 4712 - 4720

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.049

Molecular Nutrition

Effects of Dietary β-Glucan on Growth Performance and Intestinal Microflora of Weaned Piglets

Expand

Biological Feed and Molecular Nutrition Laboratory, College of Animal Science, Southwest University, Chongqing 400715, China

Received date: 2018-04-26

Online published: 2018-11-20

Fold

Abstract

This experiment was conducted to investigate the effects of β-glucan instead of antibiotics on growth performance, intestinal microflora and microbial amino acid decarboxylase activity of weaned piglets. Thirty 28-day-old healthy weaned piglets[(Rongchang×Yorkshire)×Landrace] were randomly divided into 3 groups with 10 replicates per group and 1 pig in each replicate. Pigs in the control group were fed a basal diet, those in the bacitracin zinc group were fed the basal diet supplemented with 100 mg/kg bacitracin zinc, and those in the β-glucan group were fed the basal diet supplemented with 400 mg/kg β-glucan. The experiment lasted for 28 days after 4 days adaption. The results showed that, compared with the control group, the growth performance and diarrhea index of weaned piglets in the β-glucan group and bacitracin zinc group were significantly increased (P<0.05), and the ratios of feed to gain in above two groups were significantly decreased (P<0.05). No significant differences were found in growth performance, diarrhea index and the ratio of feed to gain between the β-glucan group and bacitracin zinc group (P>0.05). Dietary β-glucan significantly reduced the activities of microbial tryptophan decarboxylase and lysine decarboxylase in ileum of weaned piglets compared with the bacitracin zinc (P<0.05). The activities of microbial methionine decarboxylase, ornithine decarboxylase and lysine decarboxylase in colon of weaned piglets in the β-glucan group and bacitracin zinc group were significantly decreased (P<0.05), while the tryptophan decarboxylase activity was significantly improved (P<0.05) compared with the control group. Dietary β-glucan didn't alter the dominant microbial flora in the colon, while improved the relative abundance of Lactobacillus, Veillonellaceae, Bacteroidetes, Firmicutes and Lachnospiraceae and reduced the relative abundance of harmful bacterium such as Clostridium, and this effect was equivalent to bacitracin zinc. In conclusion, supplementation with 400 mg/kg β-glucan can improve growth performance, reduce intestinal decarboxylase activity, improve the relative abundance of microorganisms and decrease the relative abundance of harmful bacterium in the colon of weaned piglets. The effects of β-glucan on promoting probiotics growth is better than bacitracin zinc.

Key words： β-glucan; weaned piglets; intestinal microflora; decarboxylase

Cite this article

CHEN Qingju, LIU Jinyan, LU Changwen, MA Yajun, TANG Zhiru . Effects of Dietary β-Glucan on Growth Performance and Intestinal Microflora of Weaned Piglets[J]. Chinese Journal of Animal Nutrition, 2018 , 30(11) : 4712 -4720 . DOI: 10.3969/j.issn.1006-267x.2018.11.049

References

[1] 王书凤,龚月生.不同抗生素组合对哺乳仔猪生产性能及肠道菌群的影响[J].西北农业学报,2007,16(5):63-66.

[2] LOOFT T,ALLEN H K,CANTAREL B L,et al.Bacteria,phages and pigs:the effects of in-feed antibiotics on the microbiome at different gut locations[J].The ISME Journal,2014,8(8):1566-1576.

[3] 刘灿,姜燕飞,张召锋,等.燕麦β-葡聚糖对2型糖尿病大鼠肠黏膜屏障的影响[J].食品科学,2016,37(11):167-173.

[4] 管骁,姚惠源,李景军,等.燕麦β-葡聚糖研究进展[J].食品科学,2009,30(15):231-237.

[5] 李万坤,闫鸿斌,才学鹏,等.β-葡聚糖的免疫增强作用机理研究进展[J].中国畜牧兽医,2007,34(7):151-155.

[6] ZHOU T X,JUNG J H,ZHANG Z F,et al.Effect of dietary β-glucan on growth performance,fecal microbial shedding and immunological responses after lipopolysaccharide challenge in weaned pigs[J].Animal Feed Science and Technology,2013,179(1/2/3/4):85-92.

[7] SHAO Y, LEI Z, YUAN J, et al.Effect of zinc on growth performance,gut morphometry,and cecal microbial community in broilers challenged with Salmonella enterica,serovar typhimurium[J].Journal of Microbiology,2014,52(12):1002-1011.

[8] 赖星,石宝石,刘金艳,等.日粮蛋白水平对生长育肥猪肠道微生物酶活性的影响[J].中国兽医学报,2017,37(2):327-334.

[9] 唐志如,邓欢,孙志洪,等.一种快速定量检测氨基酸脱羧酶的比色方法:中国,104374768A[P].2015-02-25.

[10] KIM H B,ISAACSON R E.The pig gut microbial diversity:understanding the pig gut microbial ecology through the next generation high throughput sequencing[J].Veterinary Microbiology,2015,177(3/4):242-251.

[11] SCHLOSS P D,WESTCOTT S L,RYABIN T,et al.Introducing mothur:open-source,platform-independent,community-supported software for describing and comparing microbial communities[J].Applied and Environmental Microbiology,2009,75(23):7537-7541.

[12] ASCHENBACH J R,SCHWELBERGER H G,AHRENS F,et al.Histamine inactivation in the colon of pigs in relationship to abundance of catabolic enzymes[J].Scandinavian Journal of Gastroenterology,2006,41(6):712-719.

[13] VOLTOLINI C,BATTERSBY S,ETHERINGTON S L,et al.A novel antiinflammatory role for the short-chain fatty acids in human labor[J].Endocrinology,2012,153(1):395-403.

[14] HOOPER L V,LITTMAN D R,MACPHERSON A J.Interactions between the microbiota and the immune system[J].Science,2012,336(6086):1268-1273.

[15] SEKIROV I,RUSSELL S L,ANTUNES L C M,et al.Gut microbiota in health and disease[J].Physiological Reviews,2010,90(3):859-904.

[16] El KAOUTARI A,ARMOUGOM F,GORDON J I,et al.The abundance and variety of carbohydrate-active enzymes in the human gut microbiota[J].Nature Reviews Microbiology,2013,11(7):497-504.

[17] 周怿,刁其玉,屠焰,等.酵母β-葡聚糖和抗生素对早期断奶犊牛生长性能和肠道菌群的影响[J].畜牧兽医学报,2010,41(6):685-691.

[18] 王忠,强文军,呙于明,等.β-1,3-葡聚糖对感染沙门菌鸡免疫功能和肠道菌群数量的影响[J].中国家禽,2010,32(5):14-18.

[19] HAYASHI H,SHIBATA K,SAKAMOTO M,et al.Prevotella copri sp. nov. and Prevotella stercorea sp. nov.,isolated from human faeces[J].International Journal of Systematic and Evolutionary Microbiology,2007,57(Pt 5):941-946.

[20] AZCARATE-PERIL M A,FOSTER D M,CADENAS M B,et al.Acute necrotizing enterocolitis of preterm piglets is characterized by dysbiosis of ileal cosa-assocmuiated bacteria[J].Gut Microbes,2011,2(4):234-243.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References