Molecular Nutrition

Effects of Probiotics on Growth Performance, Serum Biochemical Indexes and Intestinal Function of Squabs

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  • 1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;
    2. Institute of Animal Husbandry and Veterinary Sciences, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    3. Huzhou Huajia Special Breeding Ltd., Huzhou 313000, China;
    4. Hangzhou Kangdequan Feed Ltd., Hangzhou 311107, China

Received date: 2018-05-02

  Online published: 2018-11-20

Abstract

This study was designed to investigate the effects of Clostridium butyrate and Lactobacillus on growth performance, serum biochemical indexes, intestinal digestive enzymes activities and intestinal morphology of squabs. Eight-four pairs of pigeons during the same breeding period and 252 1-day-old squabs were selected, and 3 squabs were fed by 1 pair of pigeons. The pigeons and squabs were randomly divided into 7 groups with 6 replicates per group and 2 pairs of pigeons and 6 squabs per replicate. Pigeons in control group (group A) were fed a basal diet, those in antibiotic group (group B) were fed the basal diet supplemented with 150 mg/kg chlortetracycline, and the others in experimental groups (groups C, D, E, F and G) were fed the basal diets supplemented with 5×107, 1×108, 2×108 CFU/kg Clostridium butyrate, 5×109 CFU/kg Lactobacillus and 5×109 CFU/kg Lactobacillus+1×108 CFU/kg Clostridium butyrate, respectively. The experiment lasted for 28 days. The results showed as follows:1) average daily gain at 15 to 28 days of age and 1 to 28 days of age and body weight at 28 days of age of squabs in group G were significantly higher than those in control group (P<0.05), but there were no significant differences between group G and antibiotic group (P>0.05). 2) Compared with control group and antibiotic group, Clostridium butyrate and Lactobacillus significantly increased the contents of total protein (TP) and albumin (ALB) in serum and significantly decreased the content of total cholesterol (TC) in serum of squabs except for the contents of TP and TC in serum in group E (P<0.05). The contents of triglyceride (TG) and urea nitrogen (UN) in serum in group G were significantly lower than those in control group (P<0.05). 3) Compared with control group and antibiotic group, the activities of trypsin, lipase and amylase in duodenal contents of squabs in group G and the activity of amylase in duodenal contents in group C were significantly increased (P<0.05). 4) Compared with control group, villus height and the ratio of villus height to crypt depth (V/C) of duodenum, jejunum and ileum of squabs in group G were significantly increased (P<0.05), and the crypt depth of duodenum, jejunum and ileum in group G was significantly decreased (P<0.05). Compared with control group, the mucosal thickness of duodenum in groups D and G was significantly increased (P<0.05), and the crypt depth of jejunum and ileum in groups C and D was significantly decreased (P<0.05). Compared with antibiotic group, the mucosal thickness of duodenum and V/C of jejunum and ileum in group G were significantly increased (P<0.05). In conclusion, adding Clostridium butyrate and Lactobacillus in pigeons diets can promote growth, improve serum biochemical indexes, increase the activities of intestinal digestive enzymes and improve intestinal morphology of squabs. Under present experimental condition, the effects of compound addition of Clostridium butyrate and Lactobacillus are better than those of single addition, and group D has the best effect among single addition.

Cite this article

YUAN Wenhua, LI Guoqin, ZHAO Wenwen, YUAN Huikun, ZHOU Xueqin, LI Zhefeng, HOU Jia, LU Lizhi, DIAO Xinping . Effects of Probiotics on Growth Performance, Serum Biochemical Indexes and Intestinal Function of Squabs[J]. Chinese Journal of Animal Nutrition, 2018 , 30(11) : 4721 -4728 . DOI: 10.3969/j.issn.1006-267x.2018.11.050

References

[1] SALEHA A A,MYAING T T,KANNAN G,et al.Possible effect of antibiotic-supplemented feed and environment on the occurrence of multiple antibiotic resistant Escherichia coli in chickens[J].International Journal of Poultry Science,2009,8(1):28-31.  

[2] 张秀江,胡虹,张永战,等.丁酸梭菌对仔猪和生长肥育猪生产性能的影响研究[J].河南科学,2015,33(10):1745-1749.

[3] 张刚.乳酸细菌-基础、技术和应用[M].北京:化学工业出版,2007:2-11.

[4] HIGGINS S E,HIGGINS J P,WOLFENDEN A D,et al.Evaluation of a Lactobacilus-based probiotic culture for the reduction of Salmonella enteritidis in neonatal broiler chicks[J].Poultry Science,2008,87(1):27-31.  

[5] 于娜,郑捷,朱振元,等.黄芪渣虫草发酵物对白羽王鸽免疫活性的影响[J].天津科技大学学报,2017,32(2):13-18.

[6] 李龙.藏鸡源乳酸菌和红景天对肉鸡生产性能、腹水敏感性和肠道功能的影响[D].博士学位论文.杨凌:西北农林科技大学,2015.

[7] 雷燕.益生菌对肉鸡生产性能、消化道生理及肠道微生物区系的影响[D].硕士学位论文.雅安:四川农业大学,2009.

[8] FRECE J,KOS B,SVETEC I K,et al.Synbiotic effect of Lactobacillus helveticus M92 and prebiotics on the intestinal microflora and immune system of mice[J].Journal of Dairy Research,2009,76(1):98-104.  

[9] 陈晓帅,杨海明,孟俊,等.复合益生菌对乳鸽生长性能、屠宰性能、免疫器官指数和血清生化指标的影响[J].动物营养学报,2017,29(7):2384-2390.

[10] YANG C M,CAO G T,FERKET P R,et al.Effects of probiotic,Clostridium butyricum,on growth performance,immune function,and cecal microflora in broiler chickens[J].Poultry Science,2012,91(9):2121-2129.  

[11] 贾志新.丁酸梭菌对樱桃谷肉鸭生长性能、免疫和抗氧化功能及肠道食糜VFA含量的影响[D].硕士学位论文.南京:南京农业大学,2014.

[12] COLLADO M C,SURONO I S,MERILUOTO J,et al.Potential probiotic characteristics of Lactobacillus and Enterococcus strains isolated from traditional Dadih fermented milk against pathogen intestinal colonization[J].Journal of Food Protection,2007,70:700-705.

[13] 陈晓帅,杨海明,孟俊,等.益生菌对乳鸽生长性能、屠宰性能、免疫器官指数和血清生化指标的影响[J].中国饲料,2017(9):26-29,34.

[14] 王杰,艾萍萍,刁其玉,等.复合益生菌和纤维寡糖对断奶仔猪生长性能、粪便微生物及血清指标的影响[J].动物营养学报,2016,28(3):881-890.

[15] DHANALAKSHMI S,DEVI R S,SRIKUMAR R,et al.Protective effect of Triphala on cold stress-induced behavioral and biochemical abnormalities in rats[J].Pharmaceutical Journal,2007,127(11):1863-1867.

[16] 贾聪慧,杨彩梅,曾新福,等.丁酸梭菌对肉鸡生长性能、抗氧化能力、免疫功能和血清生化指标的影响[J].动物营养学报,2016,28(3):908-915.

[17] 林冬梅,祝国强,李玉兰,等.复合益生菌制剂对蛋种鸡血清生化指标的影响[J].中国家禽,2009(8):22-24.

[18] LIU H L,YANG C J,JING Y,et al.Ability of lactic acid bacteria isolated from mink to remove cholesterol:in vitro and in vivo studies[J].Canadian Journal of Microbiology,2013,59(8):563-569.  

[19] 陈晓帅,杨海明,孟俊,等.复合益生菌制剂对乳鸽生长性能、脏器指数和血清生化指标的影响[J].江苏农业科学,2017,45(15):150-152.

[20] 罗辉,周剑,叶华.微生态制剂对鱼类肠道结构和消化酶活性的影响[J].水产科学,2006,25(2):105-108.

[21] JIN L Z,HO Y W,ABDULLAH N,et al.Digestive and bacterial enzyme activities in broilers fed diets supplemented with Lactobacillus cultures[J].Poultry Science,2000,79(6):886-891.  

[22] ZIAEI-NEJAD S,REZAEI M H,TAKAMI G A,et al.The effect of Bacillus spp. bacteria used as probiotics on digestive enzyme activity,survival and growth in the Indian white shrimp Fenneropenaeus indicus[J].Aquaculture,252(2/3/4):516-524.

[23] 段颖,赵玉鑫,潘翠玲,等.富硒益生菌对蛋鸡抗氧化能力和肠道菌群及消化酶活性的影响[J].南京农业大学学报,2010,33(2):68-72.

[24] 岳晓敬,扶雄锋,胡栾莎,等.复合益生菌发酵豆粕对断奶仔猪肠道形态和消化酶活性的影响[J].中国畜牧杂志,2016,52(11):49-54.

[25] AIDY S E,VAN DEN BOGERT B,KLEEREBEZEM M.The small intestine microbiota,nutritional modulation and relevance for health[J].Current Opinion in Biotechnology,2015,32:14-20.

[26] MAGALHAES J G,TATTOLI I,GIRARDIN S E.The intestinal epithelial barrier:how to distinguish between the microbial flora and pathogens[J].Seminars in Immunology,2007,19(2):106-115.  

[27] 周勃.枯草芽孢杆菌对肉鸡小肠黏膜组织形态结构的影响[J].国外畜牧学(猪与禽),2017,37(8):75-77.

[28] 秦红.益生菌对育肥猪肠道屏障功能的影响[D].硕士学位论文.太谷:山西农业大学,2016.

[29] SAMANYA M,YAMAUCHI K E.Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var. Natto[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2002,133(1):95-104.  

[30] FENG J,LIU X,XU Z R,et al.Effect of fermented soybean meal on intestinal morphology and digestive enzyme activities in weaned piglets[J].Digestive Diseases and Sciences,2007,52(8):1845-1850.  
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