Effects of Selenomethionine on Growth Performance, Tissue Selenium Content, Antioxidant Capacity and Serum Biochemical Indices of Centropristis striata

ZHANG Qingwen, CHEN Chao, SHAO Yanxiang, ZHAO Tingting

doi:10.3969/j.issn.1006-267x.2018.11.053

2018 , Vol. 30 >Issue 11: 4746 - 4756

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.11.053

Molecular Nutrition

Effects of Selenomethionine on Growth Performance, Tissue Selenium Content, Antioxidant Capacity and Serum Biochemical Indices of Centropristis striata

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1. Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;
2. College of Fisheries and Science, Shanghai Ocean University, Shanghai 201306, China

Received date: 2018-04-27

Online published: 2018-11-20

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Abstract

This experiment was conducted to investigate the effects of selenomethionine (Se-Met) on growth performance, tissue selenium content, antioxidant capacity and serum biochemical indices of Centropristis striata. A total of 540 healthy juvenile Centropristis striata with the initial body weight of (81.62±0.75) g were randomly divided into 6 groups with 3 replicates per group and 30 fish per replicate. Fish in those groups were fed experimental diets which containing 0.09 (control group), 0.21, 0.44, 0.58, 0.77 and 1.05 mg/kg selenium, respectively. The experiment lasted for 8 weeks. The results showed as follows:1) the final body weight and weight gain rate of dietary selenium content 0.58 mg/kg group were the highest, and significantly higher than those of other groups (P<0.05). The feed conversion rate of dietary selenium content 0.58 mg/kg group was the lowest, and significantly lower than that of dietary selenium content 0.09, 0.77 and 1.05 mg/kg groups (P<0.05). 2) The muscle crude lipid content of dietary selenium content 0.77 mg/kg group was the highest, and significantly higher than that of other groups except dietary selenium content 0.58 mg/kg group (P<0.05). The muscle crude ash content of dietary selenium content 0.44 mg/kg group was the highest, and significantly higher than that of other groups (P<0.05). 3) The liver selenium content of dietary selenium content 0.77 mg/kg group was significantly higher than that of dietary selenium content 0.09, 0.21 and 0.44 mg/kg groups (P<0.05). The serum selenium content of dietary selenium content 0.58 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.09, 0.21, 0.44 and 1.05 mg/kg groups (P<0.05). 4) The serum glutathione peroxidase (GPx) activity of dietary selenium content 1.05 mg/kg group was significantly higher than that of other groups (P<0.05). The serum glutathione reductase (GR) activity of dietary selenium content 0.58 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.77 and 1.05 mg/kg groups (P<0.05). The serum catalase (CAT) activity of dietary selenium content 0.58 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.09, 0.21 and 1.05 mg/kg groups (P<0.05). The serum glutathione S-transferase (GST) activity of dietary selenium content 1.05 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.09, 0.21, 0.44 and 0.58 mg/kg groups (P<0.05). 5) The liver GPx activity of dietary selenium content 0.09 mg/kg group was the lowest, and significantly lower than that of 0.44, 0.58 and 0.77 mg/kg groups (P<0.05). The liver GR activity of dietary selenium content 0.09 mg/kg group was the lowest, and significantly lower than that of 0.44, 0.58 and 1.05 mg/kg groups (P<0.05). The liver GST activity of dietary selenium content 0.09 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.44, 0.58, 0.77 and 1.05 mg/kg groups (P<0.05). The liver superoxide dismutase (SOD) activity of dietary selenium content 0.77 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.09 and 1.05 mg/kg groups (P<0.05). The liver CAT activity of dietary selenium content 0.44 mg/kg group was the highest, and significantly higher than that of other groups (P<0.05). 6) The serum total protein content of dietary selenium content 0.58 mg/kg group was the highest, and significantly higher than that of other groups (P<0.05). The serum total cholesterol content of dietary selenium content 0.58 mg/kg group was the highest, and significantly higher than that of dietary selenium content 0.09, 0.21 and 0.44 mg/kg groups (P<0.05). The serum immunoglobulin M content and lysozyme activity of dietary selenium content 0.77 mg/kg group was the highest, and significantly higher than those of dietary selenium content 0.09, 0.21, 0.44 and 1.05 mg/kg groups (P<0.05). Base on weight gain rate, liver GPX activity and serum lysozyme activity as evaluating indicators by quadratic curve analysis, the dietary selenium requirement of Centropristis striata is 0.62, 0.92 and 0.72 mg/kg, respectively, and the suitable dietary selenium content is 0.62 to 0.92 mg/kg.

Key words： Centropristis striata; selenomethionine; growth performance; tissue selenium content; antioxidant capacity

Cite this article

ZHANG Qingwen, CHEN Chao, SHAO Yanxiang, ZHAO Tingting . Effects of Selenomethionine on Growth Performance, Tissue Selenium Content, Antioxidant Capacity and Serum Biochemical Indices of Centropristis striata[J]. Chinese Journal of Animal Nutrition, 2018 , 30(11) : 4746 -4756 . DOI: 10.3969/j.issn.1006-267x.2018.11.053

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