This experiment was conducted to study the effects of Bacillus subtilis-zinc on growth development, organ index, nutrient utilization and organ trace elements (zinc, copper and iron) content of congenital zinc deficiency rats, and to determine the feeding effects of Bacillus subtilis-zinc and feasibility of the trace elements reduction. The same pregnancy period rats were selected for the model establishment experiment of congenital zinc deficiency rats, rats in the model group were fed a low zinc diet (zinc level was 13 mg/kg) and rats in the control group were fed a normal diet (zinc level 38 mg/kg), from 10 days after pregnancy to the end of lactation. After that, eighteen 24-day-old normal juvenile rats from the control group in the model establishment experiment were selected as a normal group (group Ⅰ, zinc level was 13 mg/kg), and fed a normal diet; in addition, ninety 24-day-old congenital zinc deficiency juvenile rats from the model group in the model establishment experiment were selected and randomly divided into 5 experimental groups, which were zinc deficiency group (group Ⅱ, zinc level was 13 mg/kg), zinc sulfate (ZnSO₄) group (group Ⅲ, zinc level was 38 mg/kg), low-dose Bacillus subtilis-zinc group (group Ⅳ, zinc level was 15 mg/kg), mid-dose Bacillus subtilis-zinc group (group Ⅴ, zinc level was 30 mg/kg) and high-dose Bacillus subtilis-zinc group (group Ⅵ, zinc level was 45 mg/kg), respectively, and fed low zinc diets; there were 3 replicates in each group and 6 rats in each replicate. The experiment lasted for 5 weeks. The results showed as follows:1) compared with group Ⅰ, the body weight (BW) and average daily feed intake (ADFI) of group Ⅱ were significantly decreased (P<0.05 or P<0.01). Compared with group Ⅱ, the BW and ADFI of groups Ⅳ, Ⅴ and Ⅵ were significantly increased (P<0.05 or P<0.01). Compared with group Ⅲ, the BW, average body gain (ADG) and ADFI of group Ⅴ were significantly increased (P<0.05). 2) Compared with group Ⅰ, the liver index of group Ⅱ was significantly decreased (P<0.05). Compared with group Ⅱ, the heart index and liver index of groups Ⅴ and Ⅵ were significantly increased (P<0.05 or P<0.01). Compared with group Ⅲ, the heart index of group Ⅵ were significantly increased (P<0.05), the heart index and kidney index of group Ⅴ were significantly increased (P<0.05 or P<0.01), the kidney index of group Ⅳ were significantly increased (P<0.05). 3) Compared with group Ⅰ, the utilizations of crude protein (CP), crude fat (EE), crude fiber (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), calcium and zinc of group Ⅱ were significantly decreased (P<0.05 or P<0.01). Compared with group Ⅱ, the utilizations of CP, CF, EE NDF ADF, calcium and zinc of groups Ⅴ and Ⅵ were significantly increased (P<0.05 or P<0.01). Compared with group Ⅲ, the EE utilization of group Ⅳ was significantly increased (P<0.05), the utilizations of EE, CF, NDF ADF, calcium and zinc of group Ⅴ were significantly increased (P<0.01), the utilizations of EE, CF, NDF ADF and zinc of group Ⅵ were significantly increased (P<0.05 or P<0.01). 4) Compared with group Ⅰ, the nitrogen intake, deposit nitrogen and nitrogen availability of group Ⅱ were significantly decreased (P<0.05 or P<0.01). Compared with group Ⅱ, the nitrogen intake, deposit nitrogen and nitrogen availability of groups Ⅴ and Ⅵ were significantly increased (P<0.05 or P<0.01). Compared with group Ⅲ, the nitrogen intake, deposit nitrogen of group Ⅳ were significantly decreased (P<0.05), the nitrogen intake, deposit nitrogen of group Ⅴ were significantly increased (P<0.05). 5) Compared with group Ⅰ, the contents of zinc in heart, liver and kidney of group Ⅱ were significantly decreased (P<0.05 or P<0.01). Compared with group Ⅱ, the contents of zinc in heart, liver and kidney of groups Ⅴ and Ⅵ were significantly increased (P<0.05 or P<0.01). Compared with group Ⅲ, the contents of zinc in heart and kidney of group Ⅳ were significantly decreased (P<0.05), the liver zinc content of groups Ⅳ and Ⅵ was significantly decreased (P<0.05 or P<0.01). 6) Compared with group Ⅰ, the contents of copper in heart, brain and kidney of group Ⅱ were significantly decreased (P<0.05). Compared with group Ⅱ, the heart copper content of groups Ⅳ, Ⅴ and Ⅵ was significantly increased (P<0.05 or P<0.01). Compared with group Ⅲ, the brain copper content of groups Ⅳ and Ⅵ was significantly decreased (P<0.05), the heart copper content of group Ⅴ was significantly increased (P<0.05). 7) Compared with group Ⅰ, the contents of iron in heart, liver and kidney of group Ⅱ were significantly decreased (P<0.05 or P<0.01). Compared with group Ⅱ, the contents of iron in heart, liver and kidney of groups Ⅳ, Ⅴ and Ⅵ were significantly increased (P<0.05 or P<0.01). Compared with group Ⅲ, the liver iron content of groups Ⅳ, Ⅴ and Ⅵ were significantly decreased (P<0.05 or P<0.01). In conclusion, Bacillus subtilis-zinc can promote the growth and development of congenital zinc deficiency rats, improve liver index, heart index and nutrient utilization, regulate the distribution of trace elements, the effects are better than ZnSO₄, and reduce the addition amount of zinc in the diet.