Molecular Nutrition

Research Progress on Intestinal Mucus Barrier Function

Expand
  • School of Food Science & Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China

Received date: 2018-05-23

  Online published: 2018-12-19

Abstract

Mammals have many defense mechanisms in the intestinal tract to protect the body from pathogens and prevent the effects of intestinal microbiota. Intestine mucus is the first line of defense mechanisms. The mucus layer separates the bacteria from the epithelial cells by their continuous secretion and promotes the clearance of bacteria to reduce the incidence of intestinal inflammation and infection. Recent studies have found that pathogenic microorganisms have evolved mechanisms that bypass mucus protection system. This article reviewed the basic characteristics of intestinal mucus barrier and its role in the establishment of intestinal commensal microbiota and defense against the transplantation and invasion of pathogenic microorganisms. These research results would provide the reference for better understanding the role of mucous barrier in maintaining intestinal health.

Cite this article

HAN Feifei, PENG Liyuan, ZHANG Xuefei, YANG Shasha, HAN Jianzhong . Research Progress on Intestinal Mucus Barrier Function[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 4769 -4775 . DOI: 10.3969/j.issn.1006-267x.2018.12.001

References

[1] MOAL V L L,SERVIN A.The front line of enteric host defense against unwelcome intrusion of harmful microorganisms:mucins,antimicrobial peptides,and microbiota[J].Clinical Microbiology Reviews,2006,19(2):315-337.  



[2] KESIMER M,KIRKHAM S,PICKLES R J,et al.Tracheobronchial air-liquid interface cell culture:a model for innate mucosal defense of the upper airways?[J].American Journal of Physiology:Lung Cellular and Molecular Physiology,2009,296(1):L92-L100.



[3] PRZYBYLA L M,THEUNISSEN T W,JAENISCH R,et al.Matrix remodeling maintains embryonic stem cell self-renewal by activating Stat3[J].Stem Cells,2013,31(6):1097-1106.  



[4] MARTÍNEZ-AUGUSTIN O,RIVERO-GUTIÉRREZ B,MASCARAQUE C,et al.Food derived bioactive peptides and intestinal barrier function[J].International Journal of Molecular Sciences,2014,15(12):22857-22873.  



[5] 胡艳艳,刘小伟.肠道杯状细胞结构和功能的研究进展[J].国际病理科学与临床杂志,2013,33(5):424-430.



[6] MCCAULEY H A,GUASCH G.Three cheers for the goblet cell:maintaining homeostasis in mucosal epithelia[J].Trends in Molecular Medicine,2015,21(8):492-503.  



[7] DAVIS C W,DICKEY B F.Regulated airway goblet cell mucin secretion[J].Annual Review of Physiology,2008,70(1):487-512.  



[8] MOWAT A M.Anatomical basis of tolerance and immunity to intestinal antigens[J].Nature Reviews Immunology,2003,3(4):331-341.  



[9] JOHANSSON M E V,THOMSSON K A,HANSSON G C.Proteomic analyses of the two mucus layers of the colon barrier reveal that their main component,the Muc2 mucin,is strongly bound to the fcgbp protein[J].Journal of Proteome Research,2009,8(7):3549-3557.  



[10] JOHANSSON M E V,GUSTAFSSON J K,SJÖBERG K E,et al.Bacteria penetrate the inner mucus layer before inflammation in the dextran sulfate colitis model[J].PLoS One,2010,5(8):e12238.



[11] PELASEYED T,BERGSTRÖM J H,GUSTAFSSON J K,et al.The mucus and mucins of the goblet cells and enterocytes provide the first defense line of the gastrointestinal tract and interact with the immune system[J].Immunological Reviews,2014,260(1):8-20.  



[12] HATTRUP C L,GENDLER S J.Structure and function of the cell surface (tethered) mucins[J].Annual Review of Physiology,2008,70(1):431-457.  



[13] DO S I,KIM K,KIM D H,et al.Associations between the expression of mucins (MUC1,MUC2,MUC5AC,and MUC6) and clinicopathologic parameters of human breast ductal carcinomas[J].Journal of Breast Cancer,2013,16(2):152-158.  



[14] 黄春兰,曾悦.杯状细胞及肠道黏液屏障的功能研究[J].国际消化病杂志,2017,37(6):357-360.



[15] MACKIE A R,ROUND A N,RIGBY N M,et al.The role of the mucus barrier in digestion[J].Food Digestion,2012,3(1/2/3):8-15.



[16] CONE R A.Barrier properties of mucus[J].Advanced Drug Delivery Reviews,2009,61(2):75-85.  



[17] ATUMA C,STRUGALA V,ALLEN A,et al.The adherent gastrointestinal mucus gel layer:thickness and physical state in vivo[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2001,280(5):G922-G929.



[18] FLOREY H W.The secretion and function of intestinal mucus[J].Gastroenterology,1962,43:326-329.



[19] GRUBER P,RUBINSTEIN A,LI V H K,et al.Gastric emptying of nondigestible solids in the fasted dog[J].Journal of Pharmaceutical Sciences,1987,76(2):117-122.  



[20] CONE R A.Chapter 4-mucus[M]//MESTECKY J,BIENENSTOCK J,LAMM M E,et al.Mucosal Immunology.3rd ed.Amsterdam:Elsevier Academic Press,2005:49-72.



[21] LAI S K,O'HANLON E,HARROLD S,et al.Rapid transport of large polymeric nanoparticles in fresh undiluted human mucus[J].Proceedings of the National Academy of Sciences of the United States of America,2007,104(5):1482-1487.  



[22] FORSTNER G.Signal transduction packaging and secretion of mucins[J].Annual Review of Physiology,1995,57(1):585-605.  



[23] 李俊媛.结肠癌患者与结肠癌大鼠肠道菌群的比较分析[D].硕士学位论文.大连:大连医科大学,2017.



[24] 王婷婷.肠道菌群结构变化与结直肠癌发生发展关系的研究[D].博士学位论文.上海:上海交通大学,2012.



[25] ANDRIANIFAHANANA M,MONIAUX N,BATRA S K.Regulation of mucin expression:mechanistic aspects and implications for cancer and inflammatory diseases[J].Biochimica et Biophysica Acta:Reviews on Cancer,2006,1765(2):189-222.  



[26] JOHANSSON M E V,HANSSON G C.Immunological aspects of intestinal mucus and mucins[J].Nature Reviews Immunology,2016,16(10):639-649.  



[27] VENTURA M,TURRONI F,STRATI F,et al.The gut microbiota in health and disease[M]//MARCHESI J R.Human Microbiota and Microbiome.Wallingford:CABI,2014.



[28] VARUM F J O,VEIGA F,SOUSA J S,et al.An investigation into the role of mucus thickness on mucoadhesion in the gastrointestinal tract of pig[J].European Journal of Pharmaceutical Sciences,2010,40(4):335-341.  



[29] CEBRA J J.Influences of microbiota on intestinal immune system development[J].The American Journal of Clinical Nutrition,1999,69(5):1046S-1051S.



[30] SINGH M,VOHRA H,KUMAR L,et al.Induction of systemic and mucosal immune response in mice immunised with porins of Salmonella typhi[J].Journal of Medical Microbiology,1999,48(1):79-88.  



[31] JOHANSSON M E V,GUSTAFSSON J K,HOLMÉN-LARSSON J,et al.Bacteria penetrate the normally impenetrable inner colon mucus layer in both murine colitis models and patients with ulcerative colitis[J].Gut,2014,63(2):281-291.  



[32] JAKOBSSON H E,RODRÍGUEZ-PIÑEIRO A M,SCHÜTTE A,et al.The composition of the gut microbiota shapes the colon mucus barrier[J].Embo Reports,2015,16(2):164-177.  



[33] CARIO E,GERKEN G,PODOLSKY D K.Toll-like receptor 2 controls mucosal inflammation by regulating epithelial barrier function[J].Gastroenterology,2007,132(4):1359-1374.  



[34] RAKOFF-NAHOUM S,PAGLINO J,ESLAMI-VARZANEH F,et al.Recognition of commensal microflora by Toll-like receptors is required for intestinal homeostasis[J].Cell,2004,118(2):229-241.  



[35] HAMER H M,JONKERS D,VENEMA K,et al.Review article:the role of butyrate on colonic function[J].Alimentary Pharmacology & Therapeutics,2008,27(2):104-119.



[36] HANSSON G C.Role of mucus layers in gut infection and inflammation[J].Current Opinion in Microbiology,2012,15(1):57-62.  



[37] STECHER B,HAPFELMEIER S,MÜLLER C,et al.Flagella and chemotaxis are required for efficient induction of Salmonella enterica serovar Typhimurium colitis in streptomycin-pretreated mice[J].Infection and Immunity,2004,72(7):4138-4150.  



[38] BERGSTROM K S B,KISSOON-SINGH V,GIBSON D L,et al.Muc2 protects against lethal infectious colitis by disassociating pathogenic and commensal bacteria from the colonic mucosa[J].PLoS Pathogens,2010,6(5):e1000902.



[39] PHILLIPSON M,JOHANSSON M E V,HENRIKSNÄS J,et al.The gastric mucus layers:constituents and regulation of accumulation[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2008,295(4):G806-G812.



[40] HEAZLEWOOD C K,COOK M C,ERI R,et al.Aberrant mucin assembly in mice causes endoplasmic reticulum stress and spontaneous inflammation resembling ulcerative colitis[J].PLoS Medicine,2008,5(3):e54.



[41] BIRCHENOUGH G M H,NYSTRÖM E E L,JOHANSSON M E V,et al.A sentinel goblet cell guards the colonic crypt by triggering Nlrp6-dependent Muc2 secretion[J].Science,2016,352(6293):1535-1542.  



[42] PETERSSON J,SCHREIBER O,HANSSON G C,et al.Importance and regulation of the colonic mucus barrier in a mouse model of colitis[J].American Journal of Physiology:Gastrointestinal and Liver Physiology,2011,300(2):G327-G333.



[43] DEPLANCKE B,GASKINS H R.Microbial modulation of innate defense:goblet cells and the intestinal mucus layer[J].The American Journal of Clinical Nutrition,2001,73(6):1131S-1141S.



[44] BECKER S,OELSCHLAEGER T A,WULLAERT A,et al.Bacteria Regulate intestinal epithelial cell differentiation factors both in vitro and in vivo[J].PLoS One,2013,8(2):e55620.



[45] XAVIER R J,PODOLSKY D K.Unravelling the pathogenesis of inflammatory bowel disease[J].Nature,2007,448(7152):427-434.  



[46] 李冰,于岩波.肠黏液屏障在肠道中的作用[J].世界华人消化杂志,2017,25(19):1764-1771.



[47] 董文逍,曹海龙,王邦茂.杯状细胞在肠道疾病发病中作用的研究进展[J].国际消化病杂志,2015,35(4):244-246,269.



[48] STREMMEL W.Mucosal protection by phosphatidylcholine as new therapeutic concept in ulcerative colitis[J].Zeitschrift Für Gastroenterologie,2013,51(4):384-389.  



[49] DIEBEL L N,LIBERATI D M.Reinforcement of the intestinal mucus layer protects against Clostridium difficile intestinal injury in vitro[J].Journal of the American College of Surgeons,2014,219(3):460-468.  
Outlines

/