Effects of Low Protein Diets on Growth Performance and Intestinal Microbiota of Growing-Finishing Pigs

WANG Jing, WANG Sixin, LIU Hui, ZHANG Wei, ZHANG Dongyan, WANG Yamin, JI Haifeng

doi:10.3969/j.issn.1006-267x.2018.12.014

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 12: 4864 - 4873

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.12.014

Molecular Nutrition

Effects of Low Protein Diets on Growth Performance and Intestinal Microbiota of Growing-Finishing Pigs

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Beijing Academy of Agriculture and Forestry Sciences, Institute of Animal Husbandry and Veterinary Medicine, Beijing 100097, China

Received date: 2018-05-24

Online published: 2018-12-19

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Abstract

The aim of this study was to investigate the effects of low protein diet on growth performance and intestinal microbiota of growing-finishing pigs. A total of 140 growing-finishing pigs (Landrace×Large White) with initial body weight of (45.50±3.64) kg were randomly distributed into 2 groups with 5 replicates per group and 14 pigs in each replicate. The growing-fishing pigs in control group were fed a normal protein diet (protein level was 15.05%), while others in experimental group were a fed low protein diet (protein level was 12.97%). The pre-experimental period lasted for 5 days, and the experimental period lasted for 30 days. The results showed as follows: 1) the low protein diet had no significant effects on growth performance of growing-finishing pigs (P>0.05). 2)The low protein diet increased the intestinal microbiota richness and diversity of growing-finishing pigs. The microbiota composition of experimental pigs in 2 groups had less difference on the phylum level, while had great difference at genus level. Species difference analysis based on LEfSe showed that there were significant different on bacteria abundance at several taxonomic levels except phylum and class level (P<0.05). Low protein diet could significant increased the relative abundance of Lachnospiraceae, Ruminococcaceae, Butyrivibrio, Pseudobutyrivibrio which can produce butyrate by using carbohydrates and fibers (P<0.05), while significant decrease the relative abundance of Paraeggerthella and Delftia which likely cause infection (P<0.05). In addition, microbial community analysis showed that pigs in low protein diet group had different microbial community compared with pigs in normal protein diet. In conclusion, dietary protein level reduced about 2% have no negative impact on growth performance, and have beneficial effects on intestinal microbiota balance and body health.

Key words： low protein level diet; growing-finishing pigs; growth performance; intestinal microbiota

Cite this article

WANG Jing, WANG Sixin, LIU Hui, ZHANG Wei, ZHANG Dongyan, WANG Yamin, JI Haifeng . Effects of Low Protein Diets on Growth Performance and Intestinal Microbiota of Growing-Finishing Pigs[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 4864 -4873 . DOI: 10.3969/j.issn.1006-267x.2018.12.014

References

[1] KERR B J,SOUTHERN L L,BIDNER T D,et al.Influence of dietary protein level,amino acid supplementation,and dietary energy levels on growing-finishing pig performance and carcass composition[J].Journal of Animal Science,2003,81(12):3075-3087.

[2] KERR B J,MCKEITH F K,EASTER R A.Effect on performance and carcass characteristics of nursery to finisher pigs fed reduced crude protein, amino acid-supplemented diets.[J]. Journal of Animal Science, 1995, 73(2):433-40.

[3] ISAACSON R,KIM H B.The intestinal microbiome of the pig[J].Animal Health Research Reviews,2012,13(1):100-109.

[4] YANG Y X,DAI Z L,ZHU W Y.Important impacts of intestinal bacteria on utilization of dietary amino acids in pigs[J].Amino Acids,2014,doi:10.1007/s00726-014-1807-y.

[5] 范沛昕.低蛋白饲粮对断奶仔猪和育肥猪肠道微生物区系的影响[D].硕士学位论文.北京:中国农业大学,2016.

[6] ZERVAS S,ZIJLSTRA R T.Effects of dietary protein and fermentable fiber on nitrogen excretion patterns and plasma urea in grower pigs[J].Journal of Animal Science,2002,80(12):3247-3256.

[7] PRANDINI A,SIGOLO S,MORLACCHINI M,et al.Microencapsulated lysine and low-protein diets:effects on performance,carcass characteristics and nitrogen excretion in heavy growing-finishing pigs[J].Journal of Animal Science,2013,91(9):4226-4234.

[8] 邓敦.低蛋白饲粮补充必需氨基酸对猪营养生理效应的研究[D].博士学位论文.长沙:中国科学院亚热带农业生态研究所,2007.

[9] TUITOEK K,YOUNG L G,DE LANGE C F M,et al.The effect of reducing excess dietary amino acids on growing-finishing pig performance:an elevation of the ideal protein concept[J].Journal of Animal Science,1997,75(6):1575-1583.

[10] FIGUEROA J L,LEWIS A J,MILLER P S,et al.Nitrogen metabolism and growth performance of gilts fed standard corn-soybean meal diets or low-crude protein,amino acid-supplemented diets[J].Journal of Animal Science,2002,80(11):2911-2919.

[11] KIM H B,ISAACSON R E.The pig gut microbial diversity:understanding the pig gut microbial ecology through the nest generation high throughput sequencing[J].Veterinary Microbiology,2015,177(3/4):242-251.

[12] ZHANG C,ZHANG M,WANG S,et al.Interactions between gut microbiota,host genetics and diet relevant to development of metabolic syndromes in mice[J].The ISME Journal,2009,4(2):232-241.

[13] KONSTANTINOV S R,FAVIER C F,ZHU W Y,et al.Microbial diversity studies of the porcine gastrointestinal ecosystem during weaning transition[J].Animal Research,2004,53(4):317-324.

[14] 曹克飞.不同氮源对猪肠道微生物多样性及主要产蛋白酶菌株的影响[D].硕士学位论文.长春:吉林农业大学,2016.

[15] FAN P X,LIU P,SONG P X,et al.Moderate dietary protein restriction alters the composition of gut microbiota and improves ileal barrier function in adult pig model[J].Scientific Reports,2017,7:43412.

[16] SHOAIE S,KARLSSON F,MARDINOGLU A,et al.Understanding the interactions between bacteria in the human gut through metabolic modeling[J].Scientific Reports,2013,3:2532.

[17] 曾燕霞,王晶,季海峰,等.低蛋白质饲粮对育肥猪生产性能、氮代谢及血液生化指标的影响[J].家畜生态学报,2017,38(6):30-36.

[18] DAI Z L,LI X L,XI P B,et al.Metabolism of select amino acids in bacteria from the pig small intestine[J].Amino Acids,2012,42(5):1597-1608.

[19] BARRIOS C,BEAUMONT M,PALLISTER T,et al.Gut-microbiota-metabolite axis in early renal function decline[J].PLoS One,2015,10(8):e0134311.

[20] DENG Y F,LIU Y Y,ZHANG Y T,et al.Efficacy and role of inulin in mitigation of enteric sulfur-containing odor in pigs[J].Journal of the Science of Food and Agriculture,2017,97(8):2382-2391.

[21] DONOHOE D R,GARGE N,ZHANG X X,et al.The microbiome and butyrate regulate energy metabolism and autophagy in the mammalian colon[J].Cell Metabolism,2011,13(5):517-526.

[22] DOU X J,HAN J L,SONG W T,et al.Sodium butyrate improves porcine host defense peptide expression and relieves the inflammatory response upon toll-like receptor 2 activation and histone deacetylase inhibition in porcine kidney cells[J].Oncotarget,2017,8(16):26532-26551.

[23] LEE M R,HUANG Y T,LIAO C H,et al.Clinical and Microbiological characteristics of bacteremia caused by Eggerthella,Paraeggerthella,and Eubacterium species at a university hospital in Taiwan from 2001 to 2010[J].Journal of Clinical Microbiology,2012,50(6):2053-2055.

[24] WELLOCK I J,FORTOMARIS P D,HOUDIJK J G M,et al.The effect of dietary protein supply on the performance and risk of post-weaning enteric disorders in newly weaned pigs[J].Animal Science,2006,82(3):327-335.

[25] BHANDARI S K,OPAPEJU F O,KRAUSE D O,et al.Dietary protein level and probiotic supplementation effects on piglet response to Escherichia coli K88 challenge:performance and gut microbial population[J].Livestock Science,2010,133(1/2/3):185-188.

[26] ⅡNO T,MORI K,TANAKA K,et al.Oscillibacter valericigenes gen.nov.,sp.nov.,a valerate-producing anaerobic bacterium isolated from the alimentary canal of a Japanese corbicula clam[J].International Journal of Systematic and Evolutionary Microbiology,2007,57(8):1840-1845.

[27] 罗振,成艳芬,朱伟云.低蛋白饲粮对育肥猪盲肠代谢产物及菌群的影响[J].畜牧与兽医,2015,47(10):5-9.

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