Molecular Nutrition

Effects of Methionine Deficiency on Small Intestinal Development and Goblet Cells of Broilers

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  • 1. Qiandongnan Vocational & Technical College for Nationalities, Kaili 556000, China;
    2. Key Laboratory of Southwest China Wildlife Resources Conservation of Ministry of Education, College of Life Science, China West Normal University, Nanchong 637009, China

Received date: 2018-05-18

  Online published: 2018-12-19

Abstract

Aims of this research were to determine the effects of methionine (Met) deficiency on the small intestine development and goblet cells of broilers. A total of 120 one-day-old broilers with body weight of (45±5) g were divided into two groups with 6 replicates each group and 10 broilers in each replicate. Broilers in the control group were fed a basal diet, and those in the experimental group were fed Met-deficient diet for 42 days. Samples were collected on day 14, 28 and 42 and the villus height, crypt depth and the goblet cell population in different small intestine sections of broilers were detected by the light microscopy and histochemistry method. Results showed that the small intestinal villus height, crypt depth and the ratio of villus height to crypt depth were significantly or extremely significantly decreased (P<0.05 or P<0.01), and the small intestinal goblet cell amounts were also significantly or extremely significantly decreased (P<0.05 or P<0.01) in the experimental group compared with the control group. In conclusion, Met deficiency can reduce the villus height, crypt depth and the goblet cell population, indicating that the normal development and function of small intestine of broilers are finally impaired.

Cite this article

LIU Xiaodong, PENG Lilan, HE Shengmei, ZHANG Sen, SHEN Hailiang, WU Bangyuan . Effects of Methionine Deficiency on Small Intestinal Development and Goblet Cells of Broilers[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 4898 -4906 . DOI: 10.3969/j.issn.1006-267x.2018.12.017

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