Molecular Nutrition

Effects of Acute Heat Stress on Intestinal Barrier Molecule, Nutrient Transporter and Adenosine Monophosphate Activated Protein Kinase of Broilers

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  • College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China

Received date: 2018-06-16

  Online published: 2018-12-19

Abstract

The study aimed to investigate the effects of acute heat stress on the intestinal function of broilers from three aspects as intestinal barrier, nutrient transport and adenosine monophosphate activated protein kinase (AMPK) signaling pathway. A total of 80 healthy 28-day-old Arbor Acres broilers with similar weight were randomly allocated into a heat stress group and a control group with 8 replicates per group and 5 chicks per replicate. The ambient temperature in the heat stress group was (35±1) ℃ from 08:00 to 18:00 for 10 hours heat stress treatment, and the ambient temperature in the control group was (23±1) ℃. After the heat stress treatment, 1 chick per replicate was sacrificed, and jejunum mucosa and serum samples were excised. Meanwhile the growth performance, the contents of serum glucose (GLU), total cholesterol (TCHO), triglyceride (TG) and endotoxin (ET), and the relative gene expression levels of occludin, claudin-1, zonula occluden-1 (ZO-1), cationic amino acid transporters-1 (CAT-1), peptide transporter-1 (PepT-1), glucose transporter-2 (GLUT-2), liver kinase B1 (LKB1) and AMPKα1 in jejunum mucosa of broilers were detected. The results showed as follows: 1) compared with the control group, acute heat stress significantly reduced the average body weight gain and the average feed intake (P<0.05), while significantly increased the ratio of feed to gain (P<0.05) of broilers. 2) Compared with the control group, the acute heat stress significantly increased serum ET content (P<0.05), but no significant changes were detected on the contents of serum GLU, TG and TCHO (P>0.05) of broilers. 3) Compared with the control group, the relative gene expression level of ZO-1 was significantly increased after acute heat stress (P<0.05), but the acute heat stress did not significantly affect the relative gene expression levels of claudin-1 and occludin in jejunum mucosa (P>0.05) of broilers; the acute heat stress did not significantly affect the relative gene expression levels of CAT-1, PepT-1 and GLUT-2 in jejunum mucosa of broilers (P>0.05); the relative gene expression level of LKB1 in jejunum mucosa of broilers was significantly increased after acute heat stress (P<0.05), and the relative gene expression level of AMPKα1 in jejunum mucosa of broilers tended to be increased by the acute heat stress (P=0.060 3). In conclusion, the acute heat stress can significantly reduce the growth performance, damage the intestinal barrier, but do not affect the transport of amino acids, oligopeptide and GLU in intestine of broilers. Besides, the acute heat stress can affect the expression of LKB1 in jejunum mucosa of broilers.

Cite this article

ZHOU Huajin, ZHANG Hui, WANG Jiguang, HAO Yangyang, ZHONG Guang, SONG Zhigang . Effects of Acute Heat Stress on Intestinal Barrier Molecule, Nutrient Transporter and Adenosine Monophosphate Activated Protein Kinase of Broilers[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 4922 -4930 . DOI: 10.3969/j.issn.1006-267x.2018.12.019

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