This study was conducted to evaluate the effects of dietary organic zinc and organic manganese on performance, egg quality, antioxidant capacity and immune function of laying breeding hens. Five hundred and seventy-six 23-week-old healthy Hy-line brown D strain grant parent laying breeding hens with similar body weight were randomly allotted to 3 groups with 12 replicates per group and 16 hens per replicate. The hens in control group (group A) were fed a basal diet supplemented with 80 mg/kg of zinc sulphate and 80 mg/kg manganese sulfate, respectively, those in equivalent addition group (group B) were fed the basal diet with 40 mg/kg organic zinc and 40 mg/kg organic manganese replaced 40 mg/kg of zinc sulphate and 40 mg/kg manganese sulfate, respectively, and the others in extra addition group (group C) were fed the diet of control group extra supplemented with 40 mg/kg organic zinc and 40 mg/kg organic manganese, respectively. The experiment lasted for 32 weeks. The results showed as follows: 1) at the 32^nd week and 1 to 32 weeks, average egg weight of laying breeding hens in group B was significantly higher than that in control group (P<0.05). At the 24^th week, laying rate in group C was significantly higher than that in control group (P<0.05), and broken egg rate in group C was significantly lower than that in group B (P<0.05) and extremely significantly lower than that in control group (P<0.01). 2) At the 16^th week, egg shape index of laying breeding hens in group B was significantly higher than that in the other groups (P<0.05), and the content of manganese in egg yolk in group C was significantly higher than that in control group (P<0.05). At the 32^nd week, eggshell lightness in group C was significantly higher than that in group B (P<0.05), eggshell redness in groups B and C was extremely significantly higher than that in control group (P<0.01), and the content of manganese in egg yolk in group B extremely significantly higher than that in the other groups (P<0.01). 3) Dietary organic zinc and organic manganese had no significant effects on the activities of total superoxide dismutase (T-SOD), manganese superoxide dismutase (Mn-SOD), copper/zinc-superoxide dismutase (Cu/Zn-SOD) and T lymphocyte subsets proportion (CD₄, CD₈ and CD₄/CD₈) in blood and cutaneous basophilia allergy (CBA) reaction of laying breeding hens (P>0.05). In conclusion, dietary organic zinc and organic manganese can increase average egg weight and laying rate, decrease broken egg rate, increase eggshell strength, improve egg quality and increase the content of manganese in egg yolk of laying breeding hens. Under this experiment condition, half organic and half inorganic zinc and half organic manganese and half inorganic manganese have the best effects.