Effects of Methionine Hydroxy Analog Chelate Zinc on Performance, Eggshell Quality and Immune-Related Gene Expression of Laying Hens during Later Period of Laying

QI Xi, LI Jilong, MA Shuxue, LIU Xing, GAO Yupeng, MIN Yuna

doi:10.3969/j.issn.1006-267x.2018.12.021

2018 , Vol. 30 >Issue 12: 4939 - 4946

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.12.021

Molecular Nutrition

Effects of Methionine Hydroxy Analog Chelate Zinc on Performance, Eggshell Quality and Immune-Related Gene Expression of Laying Hens during Later Period of Laying

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College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China

Received date: 2018-05-28

Online published: 2018-12-19

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Abstract

This experiment was conducted to study the effects of methionine hydroxy analog chelate zinc (MHA-Zn) on performance, eggshell quality and immune-related gene expression of laying hens during later period of laying. A total of 960 Hy-Line grey laying hens (57-week-old) with similar body weight and laying rate were randomly assigned into 4 groups with 8 replicates per group and 30 hens per replicate. The corn-soybean meal basal diet (zinc content was 35.08 mg/kg) was formulated according to NRC(1994) standard for laying hens and the recommended nutrient levels of Hy-Line company, hens in the control group were fed the basal diet supplemented with 80 mg/kg zinc sulfate (as zinc), and hens in the experimental groups were fed the basal diet supplemented with 20, 40 and 80 mg/kg MHA-Zn (as zinc), respectively. The pre-experimental period lasted for 4 weeks, and the experimental period lasted for 12 weeks. The results showed as follows: 1) there were no significant differences in laying rate, average egg weight, average daily feed intake, feed to egg ratio and daily egg mass of laying hens among all groups (P>0.05). 2) There were no significant differences in albumen height, Haugh unit and yolk color of eggs among all groups (P>0.05). The eggshell strength and eggshell thickness of eggs in 40 and 80 mg/kg MHA-Zn groups were significantly higher than those in control group and 20 mg/kg MHA-Zn group (P<0.05), and the broken egg rate was significantly lower than that in control group and 20 mg/kg MHA-Zn group (P<0.05). 3) There were no significant differences in the mRNA relative expression levels of immune-related genes such as nuclear factor-κB1 (NF-κB1), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in spleen among all groups (P>0.05). The mRNA relative expression level of interleukin-8 (IL-8) in spleen in 80 mg/kg MHA-Zn group was significantly lower than that in 40 mg/kg MHA-Zn group (P<0.05), and the mRNA relative expression level of IL-8 in spleen in 40 mg/kg MHA-Zn group was significantly lower than that in 20 mg/kg MHA-Zn group (P<0.05). In conclusion, dietary supplemented with 40 and 80 mg/kg MHA-Zn can significantly increase the eggshell thickness and eggshell strength, significantly decrease the broken egg rate, and significantly decrease the mRNA relative expression level of proinflammatory factor IL-8. The optimal dietary MHA-Zn level of laying hens during later period of laying is 40 mg/kg.

Key words： laying hens; MHA-Zn; performance; eggshell quality; immune-related gene

Cite this article

QI Xi, LI Jilong, MA Shuxue, LIU Xing, GAO Yupeng, MIN Yuna . Effects of Methionine Hydroxy Analog Chelate Zinc on Performance, Eggshell Quality and Immune-Related Gene Expression of Laying Hens during Later Period of Laying[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 4939 -4946 . DOI: 10.3969/j.issn.1006-267x.2018.12.021

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