Dietary Protein Requirement of Juvenile Erythroculter ilishaeformis(♀)&times;Ancherythroculter nigrocauda (♂) Hybrid F1

WU Fan, WEN Hua, LI Qing, CHEN Jian, WANG Guiying, LI Pei, SUN Yanhong, LU Xing, YANG Changgeng

doi:10.3969/j.issn.1006-267x.2018.12.033

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 12: 5052 - 5061

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.12.033

Molecular Nutrition

Dietary Protein Requirement of Juvenile Erythroculter ilishaeformis(♀)×Ancherythroculter nigrocauda (♂) Hybrid F₁

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1. Fisheries Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan 430207, China;
2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
3. Wuhan Xianfeng Aquaculture Technology company limited, Wuhan 430207, China

Received date: 2018-06-06

Online published: 2018-12-19

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Abstract

This experiment was conducted to investigate the effects of dietary protein level on growth performance, whole body composition, serum biochemical indexes and digestive enzyme activities of juvenile Erythroculter ilishaeformis(♀)×Ancherythroculter nigrocauda(♂) hybrid F₁. Fish meal, casein and glutin were used as the protein sources to formulate 6 experimental diets containing different protein levels of 25.63%, 30.28%, 35.13%, 40.26%, 45.42% and 50.53%, respectively. A total of 630 juvenile hybrid F₁ with an average body weight of (5.42±0.16) g were randomly assigned to 6 groups with 3 replicates per group and 35 fish per replicate. The experiment lasted for 56 d. The results showed as follow: 1) the weight gain rate (WGR) and specific growth rate (SGR) of the juvenile hybrid F₁ significantly increased as dietary protein level increasing (P<0.05), and then tended to stabilize when protein level was 35.13%. The feed conversion ratio (FCR) showed a decrease trend, and the FCR in 40.26% group was the lowest, and significantly lower than that in the first three groups (P<0.05). Protein efficiency ratio (PER) and protein productive value (PPV) in 50.53% group were the lowest, and significantly lower than those in the first four groups (P<0.05). 2) The dietary protein level did not significantly affect the whole-body moisture and ash contents (P>0.05), but significantly affected the crude protein and crude lipid contents (P<0.05). Body protein contents in 40.26%, 45.42% and 50.53% groups were significantly higher than those in 25.63% group (P<0.05), while the crude lipid content trended to decrease as the dietary protein level increasing. The lipid content in 50.53% group was the lowest, and significantly lower than that in other groups (P<0.05). 3) There were no significant differences in triglyceride (TG) and total cholesterol (TCHO) contents in serum among all groups (P>0.05). Serum total protein (TP) content trended to increase first and then decrease with increasing dietary protein level, that in 40.26% group was the highest, and significanlty higher than that in other groups (P<0.05). Serum aspartate aminotransferase (AST) activity in 45.24% group was the highest, and significantly higher than that in other groups except 40.26% group (P<0.05). Serum alanine aminotransferase (ALT) activity was increased initially, and then reached a plateau with increasing dietary protein level. The values in 45.42% and 50.53% groups were significantly higher than those in other groups (P<0.05). 4) The protease and amylase activities in the intestine and liver significantly affected by dietary protein level (P<0.05). While the lipase activities in the intestine and liver did not significantly affected by dietary protein level (P>0.05). Broken-line regression analysis shows that the optimal requirement of dietary protein for juvenile hybrid F₁, using WGR and PPV as a response criteria, are 36.43% and 38.81%, respectively.

Key words： juvenile hybrid F₁; protein; growth; requirement; serum biochemical index; digestive enzyme

Cite this article

WU Fan, WEN Hua, LI Qing, CHEN Jian, WANG Guiying, LI Pei, SUN Yanhong, LU Xing, YANG Changgeng . Dietary Protein Requirement of Juvenile Erythroculter ilishaeformis(♀)×Ancherythroculter nigrocauda (♂) Hybrid F₁[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 5052 -5061 . DOI: 10.3969/j.issn.1006-267x.2018.12.033

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