Vitamin C Requirement of Juvenile Centropristis striata

ZHAO Tingting, CHEN Chao, SHAO Yanxiang, ZHANG Qingwen, LIU Ling

doi:10.3969/j.issn.1006-267x.2018.12.034

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 12: 5062 - 5074

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.12.034

Molecular Nutrition

Vitamin C Requirement of Juvenile Centropristis striata

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1. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, Qingdao 266071, China;
2. College of Fishers and Life Science, Shanghai Ocean University, Shanghai 201306, China

Received date: 2018-05-28

Online published: 2018-12-19

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Abstract

This experiment was conducted to investigate the vitamin C requirement of juvenile Centropristis striata. A single factor test design was adopted in the experiment, a total of 630 juvenile Centropristis striata with average body weight of (88.34±24.10) g were selected and randomly divided into 7 groups with 3 replicates per group and 30 fishes per replicate. Fishes in the 7 groups were fed the isonitrogenous and isocaloric diets which vitamin C contents (measured values) were 10.97 (control), 74.03, 148.33, 213.64, 335.33, 618.88, 910.98 mg/kg, respectively. The effects of dietary vitamin C on growth performance, serum biochemical indices, related enzyme activities, tissue vitamin C concentrations and muscle composition of juvenile Centropristis striata were detected. The experiment lasted for 8 weeks. The results showed as follows: 1) the weight gain rate and special gain rate in 74.03 mg/kg group were significantly higher than those in 10.97, 618.88 and 910.98 mg/kg groups (P<0.05), the feed conversion ratio in 74.03 mg/kg group was significantly lower than that in 10.97 and 910.98 mg/kg groups (P<0.05), the protein efficiency ratio in 10.97 and 910.98 mg/kg groups was significantly lower than that in 74.03 and 618.88 mg/kg groups (P<0.05), the condition factor in 335.33 mg/kg group was significantly lower than that in 74.03, 148.33 and 213.64 mg/kg groups (P<0.05). 2) The serum total protein content in 10.97 and 74.03 mg/kg groups was significantly lower than that in other groups (P<0.05), the serum triglyceride content in 618.88 and 910.98 mg/kg groups was significantly lower than that in other groups (P<0.05), the serum lysozyme activity in 10.97 mg/kg group was significantly lower than that in 213.64, 335.33, 618.88 and 910.98 mg/kg groups (P<0.05). 3) The serum superoxide dismutase (SOD) activity in 335.33 mg/kg group was significantly higher than that in 10.97, 74.03 and 910.98 mg/kg groups (P<0.05), the serum total antioxidant capacity (T-AOC) in 335.33 mg/kg group was significantly higher than that in 10.97, 618.88 and 910.98 mg/kg groups (P<0.05), the serum malondialdehyde (MDA) content in 335.33 mg/kg group was significantly lower than that in 910.98 mg/kg group (P<0.05). 4) The activities of SOD and catalase (CAT) in liver in 10.97 mg/kg group were significantly lower than those in other groups (P<0.05), the liver MDA content in 148.33, 335.33 and 910.98 mg/kg was significantly lower than that in 10.97 mg/kg group (P<0.05). 5) The muscle SOD activity and T-AOC in 74.03 mg/kg group were significantly higher than those in other groups (P<0.05). 6) The serum glutamic oxalacetic transaminase (GOT) activity in 213.64, 335.33, 618.88 and 910.98 mg/kg groups was significantly lower than that in 10.97 mg/kg group (P<0.05), the liver GOT activity in 10.97 mg/kg group was significantly lower than that in other groups (P<0.05). The serum glutamic-pyruvic transaminase (GPT) activity in 10.97 and 74.03 mg/kg groups was significantly higher than that in other groups (P<0.05). 7) The serum vitamin C accumulation in 910.98 mg/kg group was significantly higher than that in other groups (P<0.05), the liver vitamin C accumulation in 910.98 mg/kg group was significantly higher than that in 10.97, 74.03, 148.33 and 213.64 mg/kg groups (P<0.05), the muscle vitamin C accumulation in 618.88 mg/kg group was significantly higher than that in 10.97, 74.03, 148.33 and 213.64 mg/kg groups (P<0.05). 8) The muscle moisture content in 74.03 mg/kg group was significantly lower than that in 10.97, 148.33 and 213.64 groups (P<0.05), the muscle collagen content in 910.98 mg/kg group was significantly higher than that in other groups (P<0.05). In conclusion, set the special gain rate, liver vitamin C accumulation and serum lysozyme activity as evaluation indexes, the broken-line regression analysis results indicate that dietary vitamin C requirements of juvenile Centropristis striata are 66.66, 309.93 and 345.11 mg/kg, respectively.

Key words： Centropristis striata; vitamin C; growth; antioxidant; vitamin C accumulation

Cite this article

ZHAO Tingting, CHEN Chao, SHAO Yanxiang, ZHANG Qingwen, LIU Ling . Vitamin C Requirement of Juvenile Centropristis striata[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 5062 -5074 . DOI: 10.3969/j.issn.1006-267x.2018.12.034

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