This experiment was conducted to determine the optimal dietary crude protein (CP) and ether extract (EE) levels for raccoon dogs during growing period, so as to provide scientific basis for diet formulation. A 3×3 completely random design was adopted, and 9 experimental diets were prepared with 3 CP levels (22%, 24% and 36%) and 3 EE levels (7.5%, 9.5% and 11.5%). All diets contained the same contents of lysine, sulfur-containing amino acids and threonine. Sixty-three 11-week-old male raccoon dogs were randomly divided into 9 groups with 7 raccoon dogs per group, and the raccoon dogs were feed separately. The experiment lasted for 56 days. The results showed as follows: 1) the final body weight, average daily gain (ADG) and feed/gain (F/G) of raccoon dogs were significantly affected by dietary EE level (P<0.05), and the raccoon dogs fed diets containing 9.5% and 11.5% of EE had significantly higher ADG than those fed diets with 7.5% of EE (P<0.05), while the F/G significantly decreased with the increase of dietary EE level (P<0.05). 2) The serum glucose content of raccoon dogs was significantly affected by dietary EE level (P<0.05), and it was significantly decreased with the increase of dietary EE level (P<0.05). The serum urea nitrogen content of raccoon dogs was significantly affected by dietary CP level (P<0.05), and the raccoon dogs fed diet containing 26% of CP had significantly higher serum urea nitrogen content than those fed diets containing 22% and 24% of CP (P<0.05), but no significant difference was found in serum urea nitrogen content between the raccoon dogs fed diets containing 24% and 22% of CP (P>0.05). The serum glucose content had the tendency to increase with the dietary CP level increasing (P=0.055). 3) The contents of serum free lysine, methionine, threonine, total essential amino acids, total nonessential amino acids and total amino acids were significantly affected by the interaction between dietary CP and EE levels (P<0.05), in which all above measured indices were higher when raccoon dogs fed diets with either 24% of CP and 7.5% of EE or 22% of CP and 9.5% of EE. The results suggest that raccoon dogs can have the better growth performance when the diet containing 22% of CP and 9.5% of EE during growing period under the precondition of balancing the contents of dietary lysine, sulfur-containing amino acids and threonine.