Molecular Nutrition

Study on Inhibitory Effects of Achyranthes bidentata Polysaccharides on Salmonella Infection in Intestinal Porcine Epithelial Cells-1

Expand
  • Hunan Co-Innovation Center of Animal Production Safety, College of Animal Science and Technology, Hunan Agriculture University, Changsha 410128, China

Received date: 2018-05-24

  Online published: 2018-12-19

Abstract

This experiment was conducted to evaluate the effects of Achyranthes bidentata polysaccharides (ABPS) on proliferation, mRNA expression of tight junction related proteins and infection of Salmonella in intestinal porcine epithelial cells-1 (IPEC-1). Adding ABPS in IPEC-1 culture medium, the final concentration of ABPS were 0 (control), 50, 100, 200, 400 μg/mL. The effects of ABPS on IPEC-1 were detected using MTT assay, quantitative real-time PCR and plate colony counting method. The results showed as follows: 1) compared with the control group, ABPS had no significant effect on IPEC-1 proliferation (P>0.05). 2) The ABPS significantly inhibited Salmonella infection in IPEC-1, with the increase of ABPS concentration, the number of infected Salmonella decreased first and then increased. The number of infected salmonella in IPEC-1 treated with 50 μg/mL ABPS was the lowest among all groups, and was significantly lower than that in control group and 200 and 400 μg/mL of ABPS groups (P<0.01). 3) Compared with the control group, 50 and 200 μg/mL of ABPS significantly up-regulated the mRNA relative expression level of tight junction related proteins (ras-related C3 botulinum toxin substrate 1, zonula occluden-1, occludin, claudin-1) in IEPC-1 (P<0.01). Above results indicate that suitable ABPS concentration can promote IEPC-1 proliferation, strengthen the barrier function of intestinal mucosa and inhibit Salmonella infection with up-regulation the mRNA expression of tight junction related proteins.

Cite this article

ZHAO Yurong, WANG Yaodong . Study on Inhibitory Effects of Achyranthes bidentata Polysaccharides on Salmonella Infection in Intestinal Porcine Epithelial Cells-1[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 5083 -5088 . DOI: 10.3969/j.issn.1006-267x.2018.12.036

References

[1] DEITCH E A.Bacterial translocation or lymphatic drainage of toxic products from the gut:what is important in human beings[J].Surgery,2002,131(3):241-244.  



[2] ZHOU Y,YUAN H R,CUI L,et al.Effects of visfatin on the apoptosis of intestinal mucosal cells in immunological stressed rats[J].Acta Histochemica,2017,119(1):26-31.  



[3] TANG X P,LIU H,YANG S F,et al.Epidermal growth factor and intestinal barrier function[J].Mediators of Inflammation,2016,2016:1927348.



[4] GONZÁLEZ-MARISCAL L,NAVA P,HERNÁNDEZ S.Critical role of tight junctions in drug delivery across epithelial and endothelial cell layers[J].Journal of Membrane Biology,2005,207(2):55-68.  



[5] SUZUKI T.Regulation of intestinal epithelial permeability by tight junctions[J].Cellular and Molecular Life Sciences,2013,70(4):631-659.  



[6] 庾庆华.肠上皮细胞紧密连接调节的研究[D].博士学位论文.南京:南京农业大学,2009.



[7] WANG B,WU G Y,ZHOU Z G,et al.Glutamine and intestinal barrier function[J].Amino Acids,2015,47(10):2143-2154.  



[8] 吴姚平,武晓丽,徐锋,等.鼠伤寒沙门氏菌对肠道屏障的破坏作用[J].南昌大学学报(理科版),2017,41(3):265-269.



[9] SHIFFLETT D E,CLAYBURGH D R,KOUTSOURIS A,et al.Enteropathogenic E. coli disrupts tight junction barrier function and structure in vivo[J].Laboratory Investigation,2005,85(10):1308-1324.  



[10] BHUNIA A K.Salmonella enterica[M]//BHUNIA A K ed.Foodborne Microbial Pathogens.New York:Springer,2018,271-287.



[11] 张娜.猪沙门氏菌病的流行病学、临床表现、诊断和防控[J].现代畜牧科技,2017(4):86.



[12] 陈清华,刘祝英,贺建华.牛膝多糖的生物学功能及作用机制研究进展[J].饲料研究,2008(9):8-11.



[13] 陈清华.牛膝多糖对猪的营养效应和免疫调控机理研究[D].博士学位论文.长沙:湖南农业大学,2008.



[14] 秦文雅.牛膝多糖对免疫应激仔猪肠道的影响及其作用机理[D].硕士学位论文.长沙:湖南农业大学,2012.



[15] 李孟伟.牛膝多糖调控仔猪肠上皮细胞免疫应激及其机理[D].硕士学位论文.长沙:湖南农业大学,2016.



[16] 朱奇,陆斌兴,覃有泉,等.沙门氏菌生物学研究进展[J].疾病监测与控制,2015(7):474-478.



[17] URIBE J H,COLLADO-ROMERO M,ZALDÍVAR-LÓPEZ S,et al.Transcriptional analysis of porcine intestinal mucosa infected with Salmonella typhimurium revealed a massive inflammatory response and disruption of bile acid absorption in ileum[J].Veterinary Research,2016,47:11.



[18] 马杰,李孟伟,王雄,等.牛膝多糖体外抑菌效果的研究[J].饲料研究,2017(20):32-34,28.



[19] BALKOVETZ D F,KATZ J.Bacterial invasion by a paracellular route:divide and conquer[J].Microbes and Infection,2003,5(7):613-619.  



[20] CHEN Y,LI D F,DAI Z L,et al.L-methionine supplementation maintains the integrity and barrier function of the small-intestinal mucosa in post-weaning piglets[J].Amino Acids,2014,46(4):1131-1142.  



[21] CAPALDO C T,POWELL D N,KALMAN D.Layered defense:how mucus and tight junctions seal the intestinal barrier[J].Journal of Molecular Medicine,2017,95(9):927-934.  



[22] WOJCIAK-STOTHARD B,TSANG L Y F,PALEOLOG E,et al.Racl and RhoA as regulators of endothelial phenotype and barrier function in hypoxia-induced neonatal pulmonary hypertension[J].American Journal of Physiology-Lung Cellular and Molecular Physiology,2006,290(6):L1173-L1182.



[23] PREDESCU D,PREDESCU S,SHIMIZU J,et al.Constitutive eNOS-derived nitric oxide is a determinant of endothelial functional integrity[J].American Journal of Physiology-Lung Cellular and Molecular Physiology,2005,289(3):L371-L381.



[24] LAMPUGNANI M G,ZANETTI A,BREVIARIO F,et al.VE-cadherin regulates endothelial actin activating Rac and increasing membrane association of Tiam[J].Molecular Biology of the Cell,2002,13(4):1175-1189.  



[25] LIU Z,TAN J L,COHEN D M,et al.Mechanical tugging force regulates the size of cell-cell junctions[J].Proceedings of the National Academy of Sciences of the United States of America,2010,107(22):9944-9994.  



[26] NORITAKE J,FUKATA M,SATO K,et al.Positive role of IQGAP 1,an effector of Rac1,in actin-meshwork formation at sites of cell-cell contact[J].Molecular Biology of the Cell,2004,15(3):1065-1076.  
Outlines

/