Effects of Asparagine on Growth Performance and Toll-Like Receptor 4 and Nucleotide Binding Oligomerization Domain Signaling Pathways of Hypothalamus-Pituitary-Adrenal Axis of Weaned Piglets Challenged by Lipopolysaccharide

CHEN Huifu, PI Dingan, LENG Weibo, WANG Xiuying, ZHU Huiling, LIU Yulan, KANG Ping

doi:10.3969/j.issn.1006-267x.2018.12.037

2018 , Vol. 30 >Issue 12: 5089 - 5098

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.12.037

Molecular Nutrition

Effects of Asparagine on Growth Performance and Toll-Like Receptor 4 and Nucleotide Binding Oligomerization Domain Signaling Pathways of Hypothalamus-Pituitary-Adrenal Axis of Weaned Piglets Challenged by Lipopolysaccharide

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Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China

Received date: 2018-05-07

Online published: 2018-12-19

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Abstract

This study was conducted to investigate the effects of asparagine (Asn) on growth performance and key gene mRNA expression in toll like receptor 4 (TLR4) and nucleotide binding oligomerization domain (NOD) signal pathways of the hypothalamus-hypophysis-adrenal (HPA) axis of weaned piglets challenged by lipopolysaccharide (LPS). Twenty-four healthy (21±2)-old-day weaning piglets (Duroc×Landrace×Yorkshire) with a body weight of (8.12+0.56) kg were selected and randomly divided into 4 groups: 1) control group (basal diet), 2) LPS group (basal diet+LPS); 3) LPS+0.5% Asn group (basal diet +0.5% Asn+LPS); 4) LPS+1% Asn group (basal diet+1.0% Asn+LPS). There were 6 replicates in each group and 1 pig in each replicate. On day 20, pigs in LPS group, LPS+0.5% Asn group and LPS+1% Asn group were injected with 100 μg/kg BW LPS, and pigs in the control group were injected with the same amount of saline. The experiment lasted for 20 days. The results showed that compared with the control group, LPS challange and dietary Asn supplementation had no significant effects on the growth performance of weaned piglets challenged by LPS (P>0.05). LPS challenge significantly increased the mRNA expression of TLR4, myeloid differentiation factor 88 (MyD88), NOD2, receptor-interacting protein serine/threonine kinases 2 (RIP2) and nuclear factor-κB (NF-κB) mRNA expression in the HPA axis (P<0.05). Dietary Asn supplementation could significantly decrease the mRNA expression of NF-κB in hypothalamus (linear, P<0.05), and had a tendency to decrease the mRNA expression of TLR4 and NOD2 in adrenal (linear, P<0.10). In addition, dietary Asn supplementation decreased the mRNA expression of tumor necrosis factor-associated factor 6 (TRAF6) in hypophysis (linear, P<0.05), and significantly increased the mRNA expression of RIP2 in adrenal (linear, P<0.05; quadratic, P<0.05). The results suggest that although Asn had no significant effects on the growth performance of weaned piglets challenged by LPS, but it can inhibit TLR4 signal pathway via decreasing the mRNA expression of NF-κB in hypothalamus.

Key words： asparagine; lipopolysaccharide; weaned piglets; hypothalamus-hypophysis-adrenal axis; TLR4; NOD

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CHEN Huifu, PI Dingan, LENG Weibo, WANG Xiuying, ZHU Huiling, LIU Yulan, KANG Ping . Effects of Asparagine on Growth Performance and Toll-Like Receptor 4 and Nucleotide Binding Oligomerization Domain Signaling Pathways of Hypothalamus-Pituitary-Adrenal Axis of Weaned Piglets Challenged by Lipopolysaccharide[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 5089 -5098 . DOI: 10.3969/j.issn.1006-267x.2018.12.037

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