Molecular Nutrition

Effects of Corticotropin-Releasing Factor on Appetite Regulation of Broilers

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  • College of Animal Science and Technology/College of Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, China

Received date: 2018-05-09

  Online published: 2018-12-19

Abstract

This experiment used the live broilers as the object of study, intravenous injection of exogenous corticotropin-releasing factor (CFR), the changes of feed intake and the expression of appetite regulation related genes in the hypothalamus and intestine were analyzed, and the effects of CFR on the appetite regulation of broilers were investigated. Twenty 1-day-old male Arbor Acres (AA) broilers with similar body weight were randomly divided into 2 groups at 7 days of age with 10 broilers per group (one cage per broiler). At 10 days of age, broilers in the experimental group were injected 1 000.0 μg/kg BW CRF through wing intravenous and broilers in the control group were injected the same dose of saline at 08:00. After 2 h injection, the feed intake was recorded, and the mRNA relative expression level of appetite related genes in the hypothalamus, duodenum, jejunum and ileum were measured by RT-PCR, such as ghrelin, corticotropin releasing factor receptor (CRFR)1, CRFR2 and growth hormone secretagogues receptor-1α (GHSR-1α). The results showed that compared with the control group, intravenous injection of CRF significantly decreased the feed intake of broilers (P<0.05); significantly increased the mRNA relative expression levels of ghrelin and CRFR1 in hypothalamus of broilers (P<0.05), but did not affect the mRNA relative expression levels of CRFR2 and GHSR-1α in hypothalamus (P>0.05); significantly increased the mRNA relative expression levels of CRFR1, CRFR2 and GHSR-1α in duodenum, jejunum and ileum of broilers (P<0.05), significantly decreased the mRNA relative expression level of ghrelin in duodenum (P<0.05), but did not affect the mRNA relative expression level of ghrelin in jejunum and ileum (P>0.05). In conclusion, intravenous injection of CRF can decrease the feed intake of broilers, induce the central ghrelin gene expression, the inhibition of appetite suppression mediated by the up-regulated expression of CRFR1, ghrelin in the hypothalamus and CRFR1, CRFR2 and GHSR-1α genes in the intestinal tract may be the cause of the decrease of feed intake.

Cite this article

ZHOU Huajin, LI Hepeng, ZHANG Hui, WANG Jiguang, SONG Zhigang . Effects of Corticotropin-Releasing Factor on Appetite Regulation of Broilers[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 5099 -5106 . DOI: 10.3969/j.issn.1006-267x.2018.12.038

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