Lipid-Lowering Effect of Green Tea Powder and Green Tea Polyphenols in Canines

LI Yu, XU Yizhong, LIANG Ting, TAO Huanqing, HE Kun, HAO Ning, ZOU Suping, FENG Shibin, WANG Xichun, ZHOU Yibin, WANG Yijun, ZHANG Jinsong, WU Jinjie, WAN Xiaochun

doi:10.3969/j.issn.1006-267x.2018.12.039

Chinese Journal of Animal Nutrition >

2018 , Vol. 30 >Issue 12: 5107 - 5117

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.12.039

Molecular Nutrition

Lipid-Lowering Effect of Green Tea Powder and Green Tea Polyphenols in Canines

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1. State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China;
2. College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2018-06-21

Online published: 2018-12-19

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Abstract

The current study aimd to study the lipid-lowering effect of green tea powder and green tea polyphenols in canines, and to reveal the mechanism of lipid-lowering effect. The experiment selected 15 healthy 5-month-old local pastoral canines, and randomly divided into 3 groups with 5 canines in each group. The canines in control group were fed a basal diet, the canines in green tea powder group were fed the basal diet supplemented with 1.00% green tea powder, and the canines in green tea polyphenols group was fed the basal diet supplemented with 0.25% green tea polyphenols. The trial lasted for 12 weeks. The results showed as follows: 1) during the whole experiment period, the body weight of 3 groups showed an increasing trend, and the body weight of green tea powder group and green tea polyphenols group were significantly lower than that of control group. Compared with the control group, the body weight of green tea powder group began to show significant difference in the 4th week (P<0.05), and remained difference until the end of the experiment (the 12th week); the body weight of green tea polyphenol group began to show significant difference in the 6th week (P<0.05), and then (the 7th, 8th, 9th and 10th week) fluctuated to no significant difference (P>0.05), and in the last two weeks (the 11th and 12th week) showed significant difference (P<0.05). 2) The contents of serum total cholesterol (TC) and low density lipoprotein cholesterin (LDL-C) in the 7th to 12th week of green tea powder group and green tea polyphenols group were significantly lower than those of control group (P<0.05); the content of serum triglyceride (TG) in the 8th to 12th week of green tea powder group and green tea polyphenols group was significantly lower than that of control group (P<0.05); the content of serum high density lipoprotein cholesterol (HDL-C) in the 6th to 12th week of green tea powder group and green tea polyphenols group was significantly higher than that of control group (P<0.05). 3) Compared with the control group, both green tea powder group and green tea polyphenols group could significantly decrease the mRNA relative expression levels of acetyl CoA carboxylase α (ACCα), fatty acid synthase (FAS) and apolipoprotein B-100 (ApoB-100) (P<0.05), and could significantly increase the mRNA relative expression levels of apolipoprotein A-1(ApoA-1), peroxisome proliferators-activated receptor α (PPARα) and acyl-CoA oxidase (ACO) in canine hepatic tissue (P<0.05). The mRNA relative expression level of fatty acid translocase (CD36) in canine hepatic tissue of green tea powder group was not significantly different from that in control group (P>0.05), and it of green tea polyphenol group was significantly higher than that of control group in canine hepatocytes (P<0.05). Under this experimental condition, the results indicate that 1.00% green tea powder or 0.25% green tea polyphenols added to the canine diets can effectively control the body weight of canines, regulate the lipid metabolism in canines, decrease the mRNA relative expression levels of ACCα, FAS and ApoB-100 in canine hepatic tissue, increase the mRNA relative expression levels of ApoA-1, CD36, PPARα and ACO in canine hepatic tissue, and play an important role in lowering lipid.

Key words： canines; green tea powder; green tea polyphenols; lowering lipid; expression of gene

Cite this article

LI Yu, XU Yizhong, LIANG Ting, TAO Huanqing, HE Kun, HAO Ning, ZOU Suping, FENG Shibin, WANG Xichun, ZHOU Yibin, WANG Yijun, ZHANG Jinsong, WU Jinjie, WAN Xiaochun . Lipid-Lowering Effect of Green Tea Powder and Green Tea Polyphenols in Canines[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 5107 -5117 . DOI: 10.3969/j.issn.1006-267x.2018.12.039

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