Effects of Eucommia ulmoides Oliver Extracts on Growth Performance, Serum Biochemical Indexes and Liver Lipid Metabolism of Ningxiang Pigs

LI Chenyan, ZHANG Yang, XIAO Dingfu, XING Yueteng, YU Yuannian, WU Xin, LIU Xingliang, XIE Xianming, ZHANG Xu, ZENG Yongbo, ZHANG Bin

doi:10.3969/j.issn.1006-267x.2018.12.041

2018 , Vol. 30 >Issue 12: 5127 - 5133

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.12.041

Molecular Nutrition

Effects of Eucommia ulmoides Oliver Extracts on Growth Performance, Serum Biochemical Indexes and Liver Lipid Metabolism of Ningxiang Pigs

Expand

1. Hunan Co-Innovation Center of Safety Animal Production, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
3. Ningxiang Animal Husbandry, Veterinary and Aquatic Products Bureau, Ningxiang 410600, China

Received date: 2018-05-22

Online published: 2018-12-19

Fold

Abstract

This study was conducted to determine the effects of dietary Eucommia ulmoides Oliver extracts (EUOE) on growth performance, serum biochemical indexes and liver lipid metabolism of Ningxiang pigs. Twenty-four healthy castrated female Ningxiang pigs with the body weight of about 43 kg were randomly allocated to 2 groups with 4 replicates per group and 3 pigs per replicate. Pigs in control group were fed a basal diet, and the others in experimental group were fed the basal diet supplemented with 1 500 mg/kg EUOE. The experiment lasted for 56 days. The results showed as follows: compared with control group, 1) dietary EUOE increased average daily gain of Ningxiang pigs slightly, decreased average daily feed intake by 46.88 g/d and the ratio of feed to gain by 2.74%, but there were no significant differences (P>0.05). 2) Dietary EUOE significantly increased the content of high-density lipoprotein (HDL) in serum of Ningxiang pigs by 20.29% (P<0.05) and tended to increase the content of triglyceride (TG) in serum by 33.33% (0.05≤P<0.10), and significantly decreased the content of low-density lipoprotein (LDL) in serum by 13.21% (P<0.05). 3) Dietary EUOE tended to increase the content of palmitoleic acid of monounsaturated fatty acids (MUFA) in liver of Ningxiang pigs by 19.60% (0.05≤P<0.10), and tented to decrease the content of linoleic acid of polyunsaturated fatty acids (PUFA) in liver by 10.71% (0.05≤P<0.10). 4) Dietary EUOE significantly down-regulated the mRNA expression levels of adipose triglyceride lipase (ATGL), acetyl-CoA carboxylase (ACC), liver X receptor α (LXRα) and fatty acid synthase (FAS) in liver of Ningxiang pigs by 62.00%, 76.00%, 77.00% and 82.00%, respectively (P<0.05). These results indicate that dietary EUOE can affect fatty acids metabolism of Ningxiang pigs by increasing the content of HDL in serum and decreasing the content of LDL in serum and down-regulating the mRNA expression levels of FAS, ACC and so on in liver.

Key words： Eucommia ulmoides Oliver extracts; growth performance; serum biochemical indexes; fatty acids; Ningxiang pig

Cite this article

LI Chenyan, ZHANG Yang, XIAO Dingfu, XING Yueteng, YU Yuannian, WU Xin, LIU Xingliang, XIE Xianming, ZHANG Xu, ZENG Yongbo, ZHANG Bin . Effects of Eucommia ulmoides Oliver Extracts on Growth Performance, Serum Biochemical Indexes and Liver Lipid Metabolism of Ningxiang Pigs[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 5127 -5133 . DOI: 10.3969/j.issn.1006-267x.2018.12.041

References

[1] ZHU M Q,SUN R C.Eucommia ulmoides Oliver:a potential feedstock for bioactive products[J].Journal of Agricultural and Food Chemistry,2018,66(22):5422-5438.

[2] HUSSAIN T,TAN B E,LIU G,et al.Health-promoting properties of Eucommia ulmoides:a review[J].Evidence-Based Complementary and Alternative Medicine,2016,2016:5202908.

[3] XIE G P,JIANG N,WANG S N,et al.Eucommia ulmoides Oliv. bark aqueous extract inhibits osteoarthritis in a rat model of osteoarthritis[J].Journal of Ethnopharmacology,2015,162:148-154.

[4] 石海仁,滚双宝,张生伟,等.杜仲叶对育肥猪生长性能、胴体性状、抗氧化能力及肠道菌群的影响[J].动物营养学报,2018,30(1):350-359.

[5] LIU H W,LI K,ZHAO J S,et al.Effects of polyphenolic extract from Eucommia ulmoides Oliver leaf on growth performance,digestibility,rumen fermentation and antioxidant status of fattening lambs[J].Animal Science Journal,2018,89(6):888-894.

[6] FENG Z M,GUO J P,KONG X F,et al.Molecular cloning and expression profiling of G protein coupled receptor 120 in Landrace pig and different Chinese indigenous pig breeds[J].Journal of Food Agriculture and Environment,2012,10(3):809-814.

[7] ZHOU Y,RUAN Z,LI X L,et al.Eucommia ulmoides Oliver leaf polyphenol supplementation improves meat quality and regulates myofiber type in finishing pigs[J].Journal of Animal Science,2016,94(9):164-169.

[8] 王建辉,贺建华,易宣,等.杜仲提取物对猪生长性能及血液指标的影响[J].饲料研究,2007,(2):1-4.

[9] 秦龙山,邢月腾,张杨,等.半胱胺对宁乡猪胴体性状和肉品质的影响[J].动物营养学报,2017,29(9):3325-3330.

[10] 喻文娟,侯静文,朱邦尚.外标-气相色谱-质谱法准确测定猪肉中的14种脂肪酸[J].分析仪器,2012(3):10-16.

[11] 赵帅,段明房,胡红伟,等.杜仲叶的功能及在养殖动物中的应用[J].饲料博览,2017(3):27-30.

[12] KIM D C,KIM D S,KIM S J,et al.Eucommiae cortex inhibits TNF-α and IL-6 through the suppression of caspase-1 in lipopolysaccharide-stimulated mouse peritoneal macrophages[J].The American Journal of Chinese Medicine,2012,40(1):135-149.

[13] LEE S D,KIM H Y,SONG Y M,et al.The effect of Eucommia ulmoides leaf supplementation on the growth performance,blood and meat quality parameters in growing and finishing pigs[J].Animal Science Journal,2009,80(1):41-45.

[14] 陈鹏,杨在宾,黄丽波,等.八角和杜仲叶提取物对断奶仔猪生长性能、血清酶活性及肝脏肿瘤坏死因子-α分布和表达的影响[J].动物营养学报,2017,29(3):874-881.

[15] WANG J P,YOO J S,KIM H J,et al.Nutrient digestibility,blood profiles and fecal microbiota are influenced by chitooligosaccharide supplementation of growing pigs[J].Livestock Science,2009,125(2/3):298-303.

[16] 杨灿,唐小武,苏文璇,等.杜仲叶乙醇粗提物对母猪血液生化指标的影响[J].中国饲料,2018(1):55-58.

[17] 郑国栋,潘永芳,黎冬明,等.杜仲叶对小鼠肝脏脂肪代谢酶活性的影响[J].中国食品学报,2014,14(11):22-26.

[18] CHOI M S,JUNG U J,KIM H J,et al.Du-zhong (Eucommia ulmoides Oliver) leaf extract mediates hypolipidemic action in hamsters fed a high-fat diet[J].The American Journal of Chinese Medicine,2008,36(1):81-93.

[19] 刘云龙.绿原酸对大鼠非酒精性脂肪肝细胞凋亡的影响[D].硕士学位论文.长沙:湖南农业大学,2015.

[20] 李庆岗,经荣斌.猪肌内脂肪酸的研究进展[J].中国畜牧兽医,2004(3):10-12.

[21] GRINDFLEK E,SUNDVOLD H,LIEN S,et al.Rapid communication:physical and genetic mapping of the peroxisome proliferator activated receptor γ (PPARγ) gene to porcine chromosome 13[J].Journal of Animal Science,2000,78(5):1391-1392.

[22] SUNDRAM K,HAYES K C,SIRU O H.Dietary palmitic acid results in lower serum cholesterol than does a lauric-myristic acid combination in normolipemic humans[J].The American Journal of Clinical Nutrition,1994,59(4):841-846.

[23] 张春娥,张惠,刘楚怡,等.亚油酸的研究进展[J].粮油加工,2010(5):18-21.

[24] 田志梅,马现永,王丽,等.长期饲喂不同蛋白质水平饲粮对猪脂肪代谢相关基因表达的影响[J].动物营养学报,2017,29(10):3761-3772.

[25] KOBAYASHI Y,HIROI T,ARAKI M,et al.Facilitative effects of Eucommia ulmoides on fatty acid oxidation in hypertriglyceridaemic rats[J].Journal of the Science of Food and Agriculture,2012,92(2):358-365.

[26] JIN C F,BO L,LIN S M,et al.Mechanism of the inhibitory effects of Eucommia ulmoides Oliv. cortex extracts (EUCE) in the CCl₄-induced acute liver lipid accumulation in rats[J].International Journal of Endocrinology,2013,2013:751854.

[27] 周艳.杜仲叶多酚提取物对猪肉品质及绿原酸缓解肝-肠损伤研究[D].博士学位论文.南昌:南昌大学,2015.

[28] 单体中,汪以真.猪不同生长阶段脂肪酸合成酶基因的表达差异[J].农业生物技术学报,2006,14(2):293-294.

[29] WATT M J,VAN DENDEREN B J W,CASTELLI L A,et al.Adipose triglyceride lipase regulation of skeletal muscle lipid metabolism and insulin responsiveness[J].Molecular Endocrinology,2008,22(5):1200-1212.

[30] HAO S,XIAO Y,LIN Y,et al.Chlorogenic acid-enriched extract from Eucommia ulmoides leaves inhibits hepatic lipid accumulation through regulation of cholesterol metabolism in HepG2 cells[J].Pharmaceutical Biology,2016,54(2):251-259.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References