Effects of Chitosan Oligosaccharide on Growth Performance, Intestinal Structure and Non-Specific Immune Function of Crucian Carp (Carassius auratus gibelio)

SUN Fei, HE Jie, YE Yuantu, CAI Chunfang, WU Ping, WU Daiwu, ZHOU Luyang, GAO Minmin, YU Nong, ZHANG Yanfang

doi:10.3969/j.issn.1006-267x.2018.12.044

2018 , Vol. 30 >Issue 12: 5153 - 5163

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.12.044

Molecular Nutrition

Effects of Chitosan Oligosaccharide on Growth Performance, Intestinal Structure and Non-Specific Immune Function of Crucian Carp (Carassius auratus gibelio)

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1. Key Laboratory of Aquatic Nutrition of Jiangsu Province, School of Biology and Basic Medical Sciences, Soochow University, Suzhou 215123, China;
2. Zhongtaihe(Beijing) Science & Technology Development Company, Beijing 100027, China

Received date: 2018-05-28

Online published: 2018-12-19

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Abstract

In order to study the effects of chitosan oligosaccharides (COS) on growth performance, intestinal structure and non-specific immune function of crucian carp (Carassius auratus gibelio), eight experimental diets were formulated which added 0, 0.02%, 0.04% and 0.06% COS into basal diets containing normal soybean oil or oxidized soybean oil, respectively, and those diets were named as CG, OO, CG-200, CG-400, CG-600, OO-200, OO-400 and OO-600, respectively. The diet which did not add COS and take normal soybean oil as oil material (CG) was set as a positive control group, and the diet which did not add COS and take oxidized soybean oil as oil material (OO) was set as a negative control group. A total of 960 crucian carp with an initial body weight of (7.60±0.05) g were randomly divided into 8 groups with 3 replicates (net cages) per group and 40 fish per replicate, and cultured in a pond net cage for 72 d. The results showed that, compared with CG group, the specific growth rate (SGR) of CG-200 group was increased by 16.20% (P<0.05), but that of CG-400 and CG-600 groups had no significant change (P>0.05); the intestinal fold height and intestinal wall thickness were increased by 86.84% and 20.45% (P<0.05), the intestinal fold width was decreased by 12.18% (P>0.05), the serum superoxide dismutase (SOD) activity was increased by 6.59% (P>0.05) and the serum malondialdehyde (MDA) content was decreased by 16.94% (P>0.05) of CG-200 group; the SGR was decreased by 8.10% (P<0.05), the intestinal fold height was decreased by 17.81%, the intestinal fold width was increased by 70.30% (P<0.05), the intestinal wall thickness was decreased by 30.33% (P>0.05), the serum SOD activity was decreased 27.35% (P<0.05) and the serum MDA content was increased by 25.72% (P<0.05) of OO group. Compared with the OO group, the SGR of OO-200, OO-400 and OO-600 groups was tend to increase (P>0.05). The intestinal fold height was increased by 18.89% (P<0.05), the intestinal fold width was decreased by 42.99% (P<0.05), the intestinal wall thickness was decreased by 38.35% (P>0.05), the serum SOD activity was increased by 40.90% (P<0.05) and the serum MDA content was decreased 16.20% (P>0.05) of OO-400 group. The fish of OO-400 group achieved the similar states in SGR, intestinal structure, serum SOD activity and MDA content as those of CG group. In this experiment, the results indicate that, the growth, intestinal structure and non-specific immunity of crucian carp can be improved by adding 0.02% COS to a 4% soybean oil routine diet. Oxidized oil in the diet has negative effects on the growth and health of crucian carp. Adding 0.04% COS to a 4% oxidized soybean oil diet can improve the negative effects which caused by oxidizing oil, and can adjust the health degree of crucian carp to the normal level.

Key words： crucian carp (Carassius auratus gibelio); chitosan oligosaccharide; oxidized oil; intestinal structure; non-specific immune indexes

Cite this article

SUN Fei, HE Jie, YE Yuantu, CAI Chunfang, WU Ping, WU Daiwu, ZHOU Luyang, GAO Minmin, YU Nong, ZHANG Yanfang . Effects of Chitosan Oligosaccharide on Growth Performance, Intestinal Structure and Non-Specific Immune Function of Crucian Carp (Carassius auratus gibelio)[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 5153 -5163 . DOI: 10.3969/j.issn.1006-267x.2018.12.044

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