Mutagenesis Breeding and Fermentation Condition Optimization of High Yield Fiber Decomposition Enzyme of Trichoderma koningii

ZHU Yuxia, GAO Aiqin, ZHANG Tieying, LIU Junli, LIU Xintong, ZHANG Zhipeng, LIAO Chaoyong

doi:10.3969/j.issn.1006-267x.2018.12.048

2018 , Vol. 30 >Issue 12: 5192 - 5201

DOI: https://doi.org/10.3969/j.issn.1006-267x.2018.12.048

Molecular Nutrition

Mutagenesis Breeding and Fermentation Condition Optimization of High Yield Fiber Decomposition Enzyme of Trichoderma koningii

Expand

1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, China;
2. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2018-05-29

Online published: 2018-12-19

Fold

Abstract

This experiment was conducted to study the mutagenesis breeding and optimal fermentation condition optimization of high yield fiber decomposition enzyme of Trichoderma koningii. High cellulose decomposition enzyme of mutant strain was obtained through the mutagenesis breeding (ultraviolet, diethyl sulfate and ultraviolet-diethyl sulfate compound mutagenesis) of Trichoderma koningii, and the fermentation conditions of enzyme production and the hydrolysis ability of straw were studied. The results showed that a mutant strain of Trichoderma koningii UH-1 was selected through ultraviolet, diethyl sulfate and ultraviolet-diethyl sulfate compound mutagenesis of the original strain of Trichoderma koningii; compared with the original strain, the xylanase activity increased by 42.7% and the cellulase activity increased by 78.5% in mutant strain. The optimization of fermentation condition were corn stalk to bran was 7 to 3, ammonium sulphate concentration was 2%, inoculation quantity was 1.1 mL, culture medium moisture was 67%, fermentation time was 84 h, potassium dihydrogen phosphate concentration was 1%. In this condition, the filter paper enzyme activity reached 0.86 U/g, cellulase activity reached 99.81 U/g, xylanase activity reached 1 150.32 U/g; however, in the original strain of Trichoderma koningii, the filter paper enzyme activity was 0.23 U/g, the cellulase activity was 46.38 U/g, the xylanase activity was 677.86 U/g. This study showed that the mutagenesis of ultraviolet-diethyl sulfuric acid is effective in mutagenesis breeding of Trichoderma koningii, thus to improve enzyme producing ability and straw hydrolysis capacity.

Key words： Trichoderma koningii; ultraviolet mutagenesis; chemical mutagenesis; screening; fermentation conditions

Cite this article

ZHU Yuxia, GAO Aiqin, ZHANG Tieying, LIU Junli, LIU Xintong, ZHANG Zhipeng, LIAO Chaoyong . Mutagenesis Breeding and Fermentation Condition Optimization of High Yield Fiber Decomposition Enzyme of Trichoderma koningii[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 5192 -5201 . DOI: 10.3969/j.issn.1006-267x.2018.12.048

References

[1] 宋大利,侯胜鹏,王秀斌,等.中国秸秆养分资源数量及替代化肥潜力[J].植物营养与肥料学报,2018,24(1):1-21.

[2] 花莉,张成,马宏瑞,等.秸秆生物质炭土地利用的环境效益研究[J].生态环境学报,2010,19(10):2489-2492.

[3] 钱玉婷.秸秆常温降解菌的筛选及其生长特性研究[D].硕士学位论文.南京:南京农业大学,2009:1-61.

[4] 陈仕伟.黑曲霉、绿色木霉的诱变选育及其在秸秆腐熟剂中的应用初探[D].硕士学位论文.武汉:华中农业大学,2013:1-59.

[5] 常娟.高效玉米秸秆生物饲料的研制及其在肉鸡生产中的应用研究[D].博士学位论文.郑州:河南农业大学,2011:1-105.

[6] 史国翠.康氏木霉的提取及发酵条件的筛选[D].硕士学位论文.延边:延边大学,2011:1-40.

[7] 李慧君.秸秆纤维素降解菌的筛选及其利用研究[D].硕士学位论文.咸阳:西北农林科技大学,2010.

[8] 冯波.木聚糖酶产生菌的筛选、发酵条件优化及其酶学性质研究[D].硕士学位论文.长沙:湖南农业大学,2016:1-55.

[9] 张铁鹰.固态生物发酵研究进展与未来发展趋势[J].饲料与畜牧,2017(17):24-30.

[10] 孙小宝,万嘉欣,曹佳雯,等.溶解性多糖单加氧酶的研究进展[J].生物工程学报,2018,34(2):177-187.

[11] 唐仁陶.溶胀素与内切纤维素酶协同水解结晶纤维素的研究[D].硕士学位论文.长春:吉林大学,2010:1-50.

[12] IKE M,PARK J Y,TABUSE M,et al.Cellulase production on glucose-based media by the UV-irradiated mutants of Trichoderma reesei[J].Applied Microbiology and Biotechnology,2010,87(6):2059-2066.

[13] LI X H,YANG H J,ROY B,et al.Enhanced cellulase production of the Trichoderma viride mutated by microwave and ultraviolet[J].Microbiological Research,2010,165(3):190-198.

[14] 李敏霞.提高木霉属纤维素酶系中β-葡萄糖苷酶活性的研究[D].硕士学位论文.济南:齐鲁工业大学,2014:1-69.

[15] 刘爽.中低温秸秆降解菌的筛选及其秸秆降解效果研究[D].硕士学位论文.北京:中国农业科学院,2011:1-61.

[16] 殷中伟.秸秆纤维素高效降解菌株的筛选及对秸秆降解效果初步研究[D].硕士学位论文.北京:中国农业科学院,2010:1-62.

[17] 颜玉兰.产木聚糖酶木霉菌株固态发酵条件研究[D].硕士学位论文.南昌:江西农业大学,2012:1-38.

[18] 刘春芬.纤维素酶高产菌株的选育及酶的分离纯化研究[D].硕士学位论文.重庆:西南农业大学,2005:1-53.

[19] 史国翠,尹文新,张敏.康氏木霉产纤维素酶固态发酵条件的研究[J].江苏农业科学,2011(1):378-380.

[20] 谢夏阳.碳氮源对不同菌种产纤维素酶影响的研究[J].科技创新导报,2012(22):4-5,8.

[21] 孙君社,李雪,董秀芹.纤维素酶高产菌株的选育及产酶条件的研究[J].北京林业大学学报,2002,24(2):83-85.

[22] 王实玉.秸秆固态发酵工艺及其产沼气效果的研究[D].硕士学位论文.武汉:湖北工业大学,2009:1-53.

[23] 孙斐,陈靠山,张鹏英.固态发酵麸皮和玉米芯生产拟康氏木霉孢子的研究[J].中国农学通报,2010,26(6):236-239.

[24] 林元山.康氏木霉AS3.2774纤维素酶系的诱导、阻遏、纯化及鉴定研究[D].博士学位论文.南宁:广西大学,2011:1-111.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References