Molecular Nutrition

Effects of Dietary Different Oil Ratios on Growth Performance, Serum Biochemical Parameters and Intestinal Morphology of Male Silver Foxes during Winter-Furring Period

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  • State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Science, Chinese Academy of Agriculture Sciences, Changchun 130112, China

Received date: 2018-05-03

  Online published: 2018-12-19

Abstract

This experiment was conducted to study the effects of dietary different oil ratios on growth performance, serum biochemical parameters and intestinal morphology of male silver foxes during winter-furring period. Forty-eight 157-day-old male silver foxes with an average body weight of (5 450±140) g were randomly divided into 4 groups with 12 replicates per group and 1 fox per replicate, and they were fed experimental diets containing 14.00% oil but with different oil ratios. In the four experimental diets, the composition and proportion of oils were 12.00% fish oil and 2.00% soybean oil (group Ⅰ), 9.38% corn oil and 4.62% soybean oil (group Ⅱ), 12.00% corn oil and 2.00% soybean oil (group Ⅲ), 1.50% fish oil and 12.50% corn oil (group Ⅳ), respectively. The experiment period was 7 days for adaption and 40 days for trial. The results showed as follows: 1) dietary different oil ratios significantly affected body length (P<0.05), but did not significantly affect final weight, average daily gain, average daily feed intake, feed/gain and fresh fur length (P>0.05). The body length of group Ⅱ was significantly higher than that of group Ⅳ (P<0.05), but had no significant difference compared with groups Ⅰ and Ⅲ (P>0.05). 2) Dietary different oil ratios significantly or extremely significantly affected serum triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) contents (P<0.05 or P<0.01), but did not significantly affect serum high density lipoprotein cholesterol (HDL-C), cholesterol (CHO) and glucose (GLU) contents (P>0.05). The serum TG content of group Ⅱ was significantly lower than that of groups Ⅰ, Ⅲ and Ⅳ (P<0.05). The serum LDL-C content of group Ⅰ was extremely significantly higher than that of groups Ⅱ, Ⅲ and Ⅳ (P<0.01). 3) Dietary different oil ratios significantly affected serum globulin (GLOB) content (P<0.05), but did not significantly affect serum total protein (TP), albumin (ALB) and urea nitrogen (UN) contents (P>0.05). The serum GLOB content of group Ⅱ was significantly higher than that of groups Ⅲ and Ⅳ (P<0.05), but no significant difference was found between groups Ⅰ and Ⅱ (P>0.05). 4) Dietary different oil ratios significantly affected serum complement 4 (C4) content (P<0.05), but did not significantly affect serum immunoglobulin A (IgA), immunoglobulin M (IgM), immunoglobulin G (IgG), complement 3 (C3), tumor necrosis factor (TNF) and interleukin-2 (IL-2) contents (P>0.05). The serum C4 content of group Ⅲ was significantly lower than that of groups Ⅰ, Ⅱ and Ⅳ (P<0.05), and there was no significant difference among groups Ⅰ, Ⅱ and Ⅳ (P>0.05). 5) Dietary different oil ratios extremely significantly affected intestinal villous height, crypt depth and villous height/crypt depth (P<0.01). The villous height of group Ⅱ was extremely significantly higher than that of groups Ⅰ, Ⅲ and Ⅳ (P<0.01), and the villous height of groups Ⅲ and Ⅳ was extremely significantly higher than that of group Ⅰ (P<0.01). The crypt depth of group Ⅰ was extremely significantly higher than that of groups Ⅱ, Ⅲ and Ⅳ (P<0.01), while no significant difference among groups Ⅱ, Ⅲ and Ⅳ (P>0.05). The villous height/crypt depth in group Ⅱ was extremely significantly higher than that of group Ⅰ (P<0.01), and significantly higher than that of group Ⅳ (P<0.05), but had no significant difference compared with group Ⅲ (P>0.05). The results of this experiment indicate that diets containing 9.38% corn oil+4.62% soybean oil as oil sources can reduce serum TG and LDL-C contents, enhance serum GLOB content, improve intestinal morphology, which is beneficial to promote the body length of male silver foxes during winter-furring period.

Cite this article

ZHONG Wei, LUO Jing, ZHANG Ting, SUN Xuyang, LI Guangyu . Effects of Dietary Different Oil Ratios on Growth Performance, Serum Biochemical Parameters and Intestinal Morphology of Male Silver Foxes during Winter-Furring Period[J]. Chinese Journal of Animal Nutrition, 2018 , 30(12) : 5210 -5220 . DOI: 10.3969/j.issn.1006-267x.2018.12.050

References

[1] COHUET G,STRUIJKER-BOUDIER H.Mechanisms of target organ damage caused by hypertension:therapeutic potential[J].Pharmacology & Therapeutics,2006,111(1):81-98.  



[2] HULVER M W,BERGGREN J R,CORTRIGHT R N,et al.Skeletal muscle lipid metabolism with obesity[J].American Journal of Physiology:Endocrinology and Metabolism,2003,284(4):E741-E747.



[3] 王新颖,黎介寿.ω-3多不饱和脂肪酸在不同疾病中的应用研究[J].肠外与肠内营养,2007,14(3):177-182.



[4] 乔立君,郑征,马文慧,等.多不饱和脂肪酸对大鼠肠道菌群及脂肪代谢相关基因的影响[J].食品科学,2014,35(17):231-235.



[5] 郭艳,苏宜香.不同脂肪酸构成比对小鼠血脂影响的实验研究[J].营养学报,2004,26(1):5-8.



[6] SHEARER G C,HARRIS W S,PEDERSEN T L,et al.Detection of omega-3 oxylipins in human plasma and response to treatment with omega-3 acid ethyl esters[J].Journal of Lipid Research,2010,51(8):2074-2081.  



[7] 段叶辉,李凤娜,李丽立,等.n-6/n-3多不饱和脂肪酸比例对机体生理功能的调节[J].天然产物研究与开发,2014,26(4):626-631,479.



[8] 钟伟,张婷,罗婧,等.饲粮n-6/n-3多不饱和脂肪酸配比对北极狐冬毛期体脂沉积、体脂肪酸组成及血液生化指标的影响[J].畜牧兽医学报,2017,48(6):1054-1065.



[9] 钟伟,张婷,罗婧,等.饲粮n-6/n-3多不饱和脂肪酸比值对冬毛期北极狐生长性能及肝脏脂肪酸代谢相关蛋白基因表达的影响[J].动物营养学报,2017,29(3):906-915.



[10] 喻礼怀.饲粮脂肪酸ω-6/ω-3对鹅脂肪代谢影响及其分子机制的研究[D].博士学位论文.扬州:扬州大学,2012:34-43.



[11] 英永.不同比例n-6/n-3不饱和脂肪酸对鸡免疫功能的影响[D].硕士学位论文.兰州:甘肃农业大学,2008:20-32.



[12] 夏兆刚,呙于明,陈士勇,等.不同n-3/n-6多不饱和脂肪酸对产蛋鸡免疫功能的影响[J].中国兽医杂志,2004,40(7):6-9.



[13] ROUVINEN K.Dietary effects of omega-3 polyunsaturated fatty acids on body fat composition and health status of farm-raised blue and silver foxes[J].Acta Agriculturae Scandinavica,1991,41(4):401-414.  



[14] 陈小燕,王友升,李丽萍.3种色谱柱对37种脂肪酸的分离性能比较及鱼油脂肪酸检测[J].食品科学,2011,32(22):156-162.



[15] WU Y B,RAVINDRAN V,THOMAS D G,et al.Influence of method of whole wheat inclusion and xylanase supplementation on the performance,apparent metabolisable energy,digestive tract measurements and gut morphology of broilers[J].British Poultry Science,2004,45(3):385-394.  



[16] 沈曼曼.ω-6、ω-3多不饱和脂肪酸及其比值对畜禽影响的研究进展[J].广东饲料,2012,21(12):32-35.



[17] 喻礼怀,王剑飞,王梦芝,等.n-6/n-3多不饱和脂肪酸不同比例对扬州鹅生产性能和屠宰性能的影响[J].中国家禽,2012,34(23):18-22.



[18] 夏中生,陈继新,谢梅冬,等.饲粮油脂对蛋鸡生产性能、血清脂质含量和蛋黄脂肪酸组成的影响[J].广西农业生物科学,2003,22(3):171-177.



[19] SIMOPOULOS A P.Evolutionary aspects of diet,the omega-6/omega-3 ratio and genetic variation:nutritional implications for chronic diseases[J].Biomedicine & Pharmacotherapy,2006,60(9):502-507.  



[20] SIMOPOULOS A P.Evolutionary aspects of diet:the omega-6/omega-3 ratio and the brain[J].Molecular Neurobiology,2011,44(2):203-215.  



[21] PIRILLO A,CATAPANO A L.Omega-3 polyunsaturated fatty acids in the treatment of atherogenic dyslipidemia[J].Atherosclerosis Supplements,2013,14(2):237-242.  



[22] ISHIDA T,OHTA M,NAKAKUKI M,et al.Distinct regulation of plasma LDL cholesterol by eicosapentaenoic acid and docosahexaenoic acid in high fat diet-fed hamsters:participation of cholesterol ester transfer protein and LDL receptor[J].Prostaglandins,Leukotrienes and Essential Fatty Acids,2013,88(4):281-288.  



[23] SCHUCHARDT J P,NEUBRONNER J,BLOCK R C,et al.Associations between omega-3 index increase and triacylglyceride decrease in subjects with hypertriglyceridemia in response to six month of EPA and DHA supplementation[J].Prostaglandins,Leukotrienes and Essential Fatty Acids,2014,91(4):129-134.  



[24] LEE S P S,DART A M,WALKER K Z,et al.Effect of altering dietary n-6:n-3 PUFA ratio on cardiovascular risk measures in patients treated with statins:a pilot study[J].British Journal of Nutrition,2012,108(7):1280-1285.  



[25] 舒晓亮,蔡东联.膳食脂肪酸及n-6/n-3比与血脂及脂质过氧化关系的研究进展[J].肠外与肠内营养,2007,14(4):246-249.



[26] 余红心,贾俊静,李琦华,等.不同蛋白质水平日粮对云南武定鸡生长性能及血液生化指标的影响[J].中国饲料,2008(5):24-26.



[27] 高士争,雷风.日粮脂肪来源对肉鸡免疫功能的影响[J].中国家禽,1999,21(3):4-6.



[28] 陈士勇,呙于明,夏兆刚,等.不同类型多不饱和脂肪酸对产蛋鸡免疫功能及肝脏脂质过氧化的影响[J].营养学报,2003,25(4):383-388.



[29] 汪鲲,高占峰,齐广海,等.富含n-3PUFA蛋黄保健作用的研究[J].饲料工业,2000,21(8):1-4.



[30] GRIMM H,TIBELL A,NORRLIND B,et al.Immunoregulation by parenteral lipids:impact of the n-3 to n-6 fatty acid ratio[J].Journal of Parenteral & Enteral Nutrition,1994,18(5):417-421.  



[31] WINZELL M S,SVENSSON H,ARNER P,et al.The expression of hormone-sensitive lipase in clonal β-cells and rat islets is induced by long-term exposure to high glucose[J].Diabetes,2001,50(10):2225-2230.  



[32] 韩正康.家畜营养生理学[M].北京:农业出版社,1993:16-17.



[33] 马爽爽.内源性ω-3 PUFAs增加对小鼠体重及肠道益生菌的影响[D].硕士学位论文.青岛:青岛大学,2016:30-33.
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