Effects of L-Arginine on Growth Performance, Immune Function and Genes Expression Levels of Tumor Necrosis Factor-&alpha; and Interferon-&gamma; in Liver and Kidney of Cold-Stressed Piglets

HUANG Siqi, QU Hongyan, HUANG Dapeng, GENG Zhongcheng

doi:10.3969/j.issn.1006-267x.2019.01.018

2019 , Vol. 31 >Issue 1: 131 - 139

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.01.018

Molecular Nutrition

Effects of L-Arginine on Growth Performance, Immune Function and Genes Expression Levels of Tumor Necrosis Factor-α and Interferon-γ in Liver and Kidney of Cold-Stressed Piglets

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1. College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China;
2. Center of Breeding and Rescuing Endangered Wildlife, Harbin 150050, China

Received date: 2018-06-29

Online published: 2019-01-16

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Abstract

The aim of this experiment was to investigate the effects of L-arginine (L-Arg) on growth performance, immune function and gene expression levels of tumor necrosis factor-α(TNF-α) and interferon-γ (IFN-γ) in the liver and kidney of cold stressed piglets. The experiment was designed with two factors with repeated complete cross factorial test. According to body weight and sexual distinction, a total of 72 piglets with 28 days of weaning and similar weight[(7.48±0.47) kg] were selected and divided into 6 groups with 6 repetitions per group and 2 piglets per repetition, and cross treated with two temperatures (normal temperature 23 to 27℃, low temperature 14 to 18℃) and three levels of L-Arg supplementation (0, 0.8% and 1.2%). The experiment lasted for 21 d. The results showed as follows:1) temperature (P=0.006) and L-Arg (P=0.027) had significant influence on the average daily gain of piglets, and their interaction could significantly increase the average daily gain of piglets (P=0.047). The temperature, L-Arg and their interaction had no significant influence on the average daily feed intake of piglets (P>0.05). Temperature (P=0.031) and L-Arg (P=0.037) had significant effects on the ratio of feed to gain, and their interaction had no significant effect on the ratio of feed to gain (P=0.269). 2) Temperature and L-Arg had significant influence on the weight of thymus and spleen of piglets (P<0.05), and their interaction had no significant effect on thymus weight (P=0.352), while significantly affected the weight of spleen (P=0.026). 3) The interaction of temperature and L-Arg had significant effects on serum immunoglobulin (Ig) A (P=0.049), IgM (P=0.021) and IgG (P=0.015) contents. 4) The interaction of temperature and L-Arg had significant effects on the gene expression level of IFN-γ in the liver (P=0.018), and had no significant effect on the gene expression levels of TNF-α and IFN-γ in the kidney (P>0.05). The results show that adding L-Arg to the diet can improve the growth performance and immune function of the cold-stressed piglets.

Key words： L-Arg; cold stress; growth performance; immune function; gene expression level

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HUANG Siqi, QU Hongyan, HUANG Dapeng, GENG Zhongcheng . Effects of L-Arginine on Growth Performance, Immune Function and Genes Expression Levels of Tumor Necrosis Factor-α and Interferon-γ in Liver and Kidney of Cold-Stressed Piglets[J]. Chinese Journal of Animal Nutrition, 2019 , 31(1) : 131 -139 . DOI: 10.3969/j.issn.1006-267x.2019.01.018

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