Effects of High Molybdenum Diet on Performance, Egg Quality, Serum Biochemical Indexes and Hepatic Antioxidative Indexes of Laying Hens during Late Laying Period

WANG Jianping, YANG Zengqiao, DING Xuemei, BAI Shiping, ZENG Qiufeng, ZHANG Keying

doi:10.3969/j.issn.1006-267x.2019.01.019

2019 , Vol. 31 >Issue 1: 140 - 147

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.01.019

Molecular Nutrition

Effects of High Molybdenum Diet on Performance, Egg Quality, Serum Biochemical Indexes and Hepatic Antioxidative Indexes of Laying Hens during Late Laying Period

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Animal Nutrition Institute, Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Key Laboratory for Animal Disease-Resistance Nutrition and Feed, Ministry of Agriculture, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2018-06-29

Online published: 2019-01-16

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Abstract

This experiment was conducted to study the effects of high molybdenum (Mo) diet on performance, egg quality, serum biochemical indexes and hepatic antioxidative indexes of laying hens during late laying period. A total of 200 pink-eggshell Lohnman laying hens (65-week-old) were randomly allotted into 4 groups with 5 replicates per group and 10 birds per replicate. Laying hens in control group were fed a basal diet (Mo content was 1.50 mg/kg), and those in other 3 groups were fed the basal diet supplemented with 10 (M10), 20 (M20), and 100 mg/kg Mo (M100) as sodium molybdate for 5 weeks. The results showed as follows:1) Mo supplementation linearly decreased laying rate (P=0.04). Compared with the control group, the laying rate in M100 group was decreased by 5.62% and the feed/egg and broken eggshell rate were increased by 11.98% and 168.59%, respectively, and the differences were significant (P<0.05). 2) Mo supplementation linearly reduced the egg thickness (P=0.04), yolk color (P=0.01) and eggshell lightness value (P=0.01), and linearly increased the eggshell redness value (P=0.04). The eggshell thickness in M20 and M100 groups was significantly decreased, and the yolk color in M100 group was significantly decreased compared with control group (P<0.05). 3) The activities of serum aspartate transaminase (AST), alanine transaminase (ALT) and alkaline phosphatase (AKP) in M100 group were significantly increased compared with control group (P<0.05), and the increase rates were 46.02%, 51.99% and 15.72%, respectively. The serum uric acid content in M100 group was significantly lower than that in other groups (P<0.05). Serum xanthine oxidase (XOD) activity showed a quadratic curve change with the Mo supplemental level increasing (P<0.05). Compared with the control group, the serum XOD activity in M10 and M20 groups was increased by 85.93% and 101.14% (P<0.05), respectively, while it was decreased by 46.64% in M100 group (P<0.05). Compared with the control group, the serum total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) activity in M100 group were significantly reduced (P<0.05), and the serum reduced glutathione (GSH) and malondialdehyde (MDA) contents were significantly increased (P<0.05). In conclusion, dietary supplemented with 10 and 20 mg/kg Mo can increase serum XOD activity without any effects on performance, egg quality and hepatic antioxidant ability of laying hens during late laying period, but when Mo supplemental level is 100 mg/kg, it can reduce the performance, egg quality, serum XOD activity and hepatic antioxidant ability of laying hens during late laying period.

Key words： molybdenum; laying hens; performance; egg quality; antioxidant ability

Cite this article

WANG Jianping, YANG Zengqiao, DING Xuemei, BAI Shiping, ZENG Qiufeng, ZHANG Keying . Effects of High Molybdenum Diet on Performance, Egg Quality, Serum Biochemical Indexes and Hepatic Antioxidative Indexes of Laying Hens during Late Laying Period[J]. Chinese Journal of Animal Nutrition, 2019 , 31(1) : 140 -147 . DOI: 10.3969/j.issn.1006-267x.2019.01.019

References

[1] SCHWARZ G,MENDEL R R,RIBBE M W.Molybdenum cofactors,enzymes and pathways[J].Nature,2009,460(7257):839-847.

[2] MARELJA Z,LEIMKVHLER S,MISSIRLIS F.Iron sulfur and molybdenum cofactor enzymes regulate the Drosophila life cycle by controlling cell metabolism[J].Frontiers in Physiology,2018,9:50.

[3] SCHWARZ G.Molybdenum cofactor biosynthesis and deficiency[J].Cellular and Molecular Life Sciences,2005,62(23):2792-2810.

[4] 郝贵增,尚泽松,爨淑楠,等.钼对AA+肉鸡生产性能、脂质代谢及抗氧化能力的影响[J].中国兽医学报,2014,34(8):1373-1376.

[5] 王佳炜.微量元素钼的生理作用及其对机体功能的影响研究进展[J].医学综述,2013,19(19):3460-3463.

[6] 呙于明.家禽营养[M].2版.北京:中国农业大学出版社,2004:134-135.

[7] 王自良,张海棠,李军民.钼的毒性与反刍动物钼中毒[J].黄牛杂志,2004(5):40-43.

[8] 郑黎.畜禽的钼中毒[J].广东畜牧兽医科技,1996,21(4):33-34.

[9] 朱重伟,张才,杨自军,等.不同钼添加水平对肉鸡生长性能和屠体性状的影响[J].湖北农业科学,2012,51(6):1195-1197.

[10] 张元贞.钼对鸡的营养作用[J].饲料与畜牧,1989,15(6):17-19.

[11] 郭荣富,戴志明,李琦华.不同钼水平日粮对雏鸭生长的影响[J].中国家禽,1992(4):36.

[12] ZHUANG Y,LIU P,WANG L Q,et al.Mitochondrial oxidative stress-induced hepatocyte apoptosis reflects increased molybdenum intake in caprine[J].Biological Trace Element Research,2016,170(1):106-114.

[13] GU X L,ALI T,CHEN R R,et al.In vivo studies of molybdenum-induced apoptosis in kidney cells of caprine[J].Biological Trace Element Research,2016,165(1):51-58.

[14] 王亚垒.高钼对绵羊骨骼的毒性作用研究[D].硕士学位论文.洛阳:河南科技大学,2013.

[15] 廖一林.钼镉联合暴露对麻鸭跖骨微观结构与及微量元素含量的影响[D].硕士学位论文.南昌:江西农业大学,2016.

[16] 王宏伟,赵静,杜祥月,等.钼对雌性小鼠骨骼形态结构的影响[J].河南科技大学学报(自然科学版),2014,35(6):71-74,79.

[17] 肖杰,杨帆,崔恒敏,等.高钼对雏鸡肝脏和脾脏抗氧化功能的影响[J].畜牧兽医学报,2010,41(7):883-890.

[18] 徐雄卫.钼镉联合诱导对鸭组织器官微量元素含量的影响[D].硕士学位论文.南昌:江西农业大学,2015.

[19] WANG J P,HE K R,DING X M,et al.Effect of feeding and withdrawal of vanadium and vitamin C on egg quality and vanadium residual over time in laying hens[J].Biological Trace Element Research,2017,177(2):367-375.

[20] YUAN Z H,ZHANG K Y,DING X M,et al.Effect of tea polyphenols on production performance,egg quality,and hepatic antioxidant status of laying hens in vanadium-containing diets[J].Poultry Science,2016,95(7):1709-1717.

[21] 夏冰,曹华斌,张彩英,等.钼镉联合诱导对麻鸭肾功能及肾Bcl-2基因mRNA表达的影响[J].中国兽医学报,2016,36(2):292-297.

[22] 张捷.不同钼水平对山羊组织自由基代谢及其金属含量的影响[D].硕士学位论文.南昌:江西农业大学,2011.

[23] JASINGE E,KULARATNAM G A M,DILANTHI H W,et al.Uric acid,an important screening tool to detect inborn errors of metabolism:a case series[J].BMC Research Notes,2017,10(1):454.

[24] 吕佳炜,殷红,李春风,等.碱性磷酸酶在骨矿化中的研究[J].黑龙江畜牧兽医,2013(1):32-34.

[25] KIERSZTAN A,WINIARSKA K,DROZAK J,et al.Differential effects of vanadium,tungsten and molybdenum on inhibition of glucose formation in renal tubules and hepatocytes of control and diabetic rabbits:beneficial action of melatonin and N-acetylcysteine[J].Molecular and Cellular Biochemistry,2004,261(1/2):9-21.

[26] BERSÉNYI A,BERTA E,KÁDÁR I,et al.Effects of high dietary molybdenum in rabbits[J].Acta Veterinaria Hungarica,2008,56(1):41-55.

[27] 杨自军,冉林武,赵树科,等.实验性钼中毒绵羊血液酶学指标的变化与锌、铜的保护作用[J].畜牧与兽医,2008,40(4):52-55.

[28] 王自良,张海棠,赵坤.微量元素钼在养鸡生产中的应用[J].中国家禽,1999,21(9):37-40.

[29] 肖杰.高钼对雏鸡肝肾影响的机理研究[D].硕士学位论文.成都:四川农业大学,2010.

[30] 王宏伟,周变华,张才,等.钼对鸡免疫功能及抗氧化能力的影响[J].中国兽医学报,2015,35(1):135-139.

[31] DEVINE P J,PERREAULT S D,LUDERER U.Roles of reactive oxygen species and antioxidants in ovarian toxicity[J].Biology of Reproduction,2012,86(2):1-10.

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