Effects of Dietary Methionine Level on Methionine Cycle Metabolism-Related Indicators in Liver and Serum of Lambs

WANG Jie, CUI Kai, WANG Shiqin, DIAO Qiyu, ZHANG Naifeng

doi:10.3969/j.issn.1006-267x.2019.01.024

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 1: 188 - 197

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.01.024

Molecular Nutrition

Effects of Dietary Methionine Level on Methionine Cycle Metabolism-Related Indicators in Liver and Serum of Lambs

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Feed Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Beijing 100081, China

Received date: 2018-06-04

Online published: 2019-01-16

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Abstract

The aim of this study was to assess the effects of dietary methionine (Met) level on methionine cycle metabolism-related indicators in liver and serum of lambs. Twelve pairs of male Hu twin lambs with an average body weight of(4.93±0.20) kg and weaned at 7 days of age were selected and divided into two groups with a matched-pairs experimental design, the experiment consisted of two stages. During the 1st stage (8 to 56 days of age), lambs were fed diets containing either sufficient[basal milk replacer+0.70% Met and basal starter+0.40% Met, control (CON) group] or deficient Met[basal milk replacer and basal starter, low Met (LM) group]. During the 2nd stage (57 to 84 days), all lambs were stop feeding milk replacer and fed the same diet containing sufficient Met (basal starter+0.40% Met). Blood samples of six twins were collected from jugular vein at 56 and 84 days of age before the lambs slaughtered, and they were slaughtered at 56 and 84 days of age for separating liver and liquid nitrogen preservation. The blood and liver samples were used to measured Met cycle metabolism-related indicators. The results showed as follows:1) the Met content and S-adenosyl methionine (SAM)/S-adenosyl-L-homocysteine (SAH) value in liver of lambs at 56 days of age in LM group were significantly higher than those in CON group (P<0.05), but the contents of Met, SAM and SAH in liver of lambs at 84 days of age in LM group were significantly lower than those in CON groups (P<0.05). 2) At 56 days of age, no significant differences were observed in the key enzyme activities of Met cycle metabolism in liver of lambs between LM and CON groups (P>0.05) except betaine-homocysteine methyltransferase (BHMT) activity, which in LM group was significantly lower than that in CON group (P<0.05); at 84 days of age, the BHMT, methionine adenosyltransferase (MAT) and glycine N-methyltransferase (GNMT) activities in liver of lambs in LM group were significantly lower than those in CON group (P<0.05). 3) Serum Met and SAM contents of lambs at 56 and 84 days of age in LM group were significantly lower than those in CON (P<0.05), but no significant differences were observed in the serum SAH and homocysteine (Hcy) contents between groups (P>0.05). The results suggest that low dietary methionine level significantly decreases the activity of BHMT in liver and the contents of Met and SAM in serum, motivates the production of Met in liver, and significantly affect the SAM/SAH value in liver, decreases the level of body methyl donor, and then affects normal methyl metabolism of lambs; recovery of dietary methionine level, methionine metabolism cycle related indicators in liver and serum fail to fully restore with Met supplementation.

Key words： lambs; methionine; methionine cycle; methyl metabolism

Cite this article

WANG Jie, CUI Kai, WANG Shiqin, DIAO Qiyu, ZHANG Naifeng . Effects of Dietary Methionine Level on Methionine Cycle Metabolism-Related Indicators in Liver and Serum of Lambs[J]. Chinese Journal of Animal Nutrition, 2019 , 31(1) : 188 -197 . DOI: 10.3969/j.issn.1006-267x.2019.01.024

References

[1] OLLIKAINEN M,SMITH K R,JOO E J,et al.DNA methylation analysis of multiple tissues from newborn twins reveals both genetic and intrauterine components to variation in the human neonatal epigenome[J].Human Molecular Genetics,2010,19(21):4176-4188.

[2] SCHNEIDER E,PLIUSHCH G,EL HAJJ N,et al.Spatial,temporal and interindividual epigenetic variation of functionally important DNA methylation patterns[J].Nucleic Acids Research,2010,38(12):3880-3890.

[3] WU X Y,CHENG J N,LU L.Vitamin B₁₂ and methionine deficiencies induce genome damage measured using the cytokinesis-block micronucleus cytome assay in human B lymphoblastoid cell lines[J].Nutrition and Cancer,2013,65(6):866-873.

[4] SAENGKERDSUB S,LINGBECK J M,WILKINSON H H,et al.Characterization of isolated yeast growth response to methionine analogs[J].Journal of Environmental Science and Health,Part B:Pesticides,Food Contaminants,and Agricultural Wastes,2013,48(12):1112-1120.

[5] MATO J M,MARTÍNEZ-CHANTAR M L,LU S C.Methionine metabolism and liver disease[J].Annual Review of Nutrition,2008,28(1):273-293.

[6] 王杰,崔凯,毕研亮,等.蛋氨酸限制与补偿对羔羊生长性能及内脏器官发育的影响[J].动物营养学报,2016,28(11):3669-3678.

[7] EMMERT J L,GARROW T A,BAKER D H.Hepatic betaine-homocysteine methyltransferase activity in the chicken is influenced by dietary intake of sulfur amino acids,choline and betaine[J].Journal of Nutrition,1996,126(8):2050-2058.

[8] PARK E I,RENDUCHINTALA M S,GARROW T A.Diet-induced changes in hepatic betaine-homocysteine methyltransferase activity are mediated by changes in the steady-state level of its mRNA[J].The Journal of Nutritional Biochemistry,1997,8(9):541-545.

[9] FINKELSTEIN J D,MARTIN J J,HARRIS B J.Effect of dietary cystine on methionine metabolism in rat liver[J].The Journal of Nutrition,1986,116(6):985-990.

[10] FINKELSTEIN J A,CHANCE W T,FISCHER J E.Brain serotonergic activity and plasma amino acid levels in genetically obese Zucker rats[J].Pharmacology Biochemistry and Behavior,1982,17(5):939-944.

[11] 余东游,许梓荣.甲基供体促仔猪生长的内分泌机制的探讨[J].中国畜牧杂志,2000,36(5):8-10.

[12] 许梓荣,占秀安.甜菜碱对肉雏鸡蛋氨酸和脂肪代谢的影响[J].畜牧兽医学报,1998,29(3):212-219.

[13] 中华人民共和国农业部.NY/T 816-2004肉羊饲养标准[S].北京:中国农业出版社,2004.

[14] 刁其玉,屠焰.一种犊牛羔羊用代乳粉:中国,02128844.5[P].2004-05-12.

[15] MIRAND P P,THERIEZ M.Amino acid requirements of preruminant lambs[J].Annales de Zootechnie,1977,26(2):287-287.

[16] 王波,柴建民,王海超,等.蛋白质水平对湖羊双胞胎公羔生长发育及肉品质的影响[J].动物营养学报,2015,27(9):2724-2735.

[17] 张丽英.饲料分析及饲料质量检测技术[M].2版.北京:中国农业大学出版社,2003.

[18] 许贵善,刁其玉,纪守坤,等.不同饲喂水平对肉用绵羊生长性能、屠宰性能及器官指数的影响[J].动物营养学报,2012,24(5):953-960.

[19] MUHAMMAD N,ABUBAKAR A A.Influence of supplemental methionine on growth performance of uda lambs in a semi-arid environment[J].International Journal of Applied Agricultural and Apicultural Research,2012,8(1):16-23.

[20] ABDELRAHMAN M M,HUNAITI D A.The effect of dietary yeast and protected methionine on performance and trace minerals status of growing Awassi lambs[J].Livestock Science,2008,115(2/3):235-241.

[21] 刘小刚.营养限制及补偿对羔羊内脏器官和血液中CD4⁺、CD8⁺ T淋巴细胞的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2010.

[22] CHILLIARD Y,BOCQUIER F,DOREAU M.Digestive and metabolic adaptations of ruminants to undernutrition and consequence on reproduction[J].Reproduction Nutrtion Development,1998,38(2):131-152.

[23] NOZIÈRE P,ATTAIX D,BOCQUIER F,et al.Effects of underfeeding and refeeding on weight and cellularity of splanchnic organs in ewes[J].Journal of Animal Science,1999,77(8):2279-2290.

[24] CAREW L B,MCMURTRY J P,ALSTER F A.Effects of methionine deficiencies on plasma levels of thyroid hormones,insulin-like growth factors-Ⅰ and -Ⅱ,liver and body weights,and feed intake in growing chickens[J].Poultry Science,2003,82(12):1932-1938.

[25] ISTASSE L,VAN EENAEME C,GÉRARD O,et al.Mechanisms of reduced and compensatory growth[J].Domestic Animal Endocrinology,2000,19(2):121-132.

[26] MATTHEWS J O,SOUTHERN L L.The effect of dietary betaine in eimeria acervulina-infected chicks[J].Poultry Science,2000,79(1):60-65.

[27] VIRTANEN E I,RUMSEY G.Betaine supplementation can optimize use of methionine,choline in diets[J].Feedstuffs,1996,68(42):12-13.

[28] PILLAI P B,FANATICO A C,BLAIR M E,et al.Homocysteine remethylation in broilers fed surfeit choline or betaine and varying levels and sources of methionine from eight to twenty-two days of age[J].Poultry Science,2006,85(10):1729-1736.

[29] DILGER R N,GARROW T A,BAKER D H.Betaine can partially spare choline in chicks but only when added to diets containing a minimal level of choline[J].The Journal of Nutrition,2007,137(10):2224-2228.

[30] BROSNAN J T,BROSNAN M E,BERTOLO R F P,et al.Methionine:a metabolically unique amino acid[J].Livestock Science,2007,112(1/2):2-7.

[31] SELHUB J,MILLER J W.The pathogenesis of homocysteinemia:interruption of the coordinate regulation by S-adenosylmethionine of the remethylation and transsulfuration of homocysteine[J].The American Journal of Clinical Nutrition,1992,55(1):131-138.

[32] 秦善春,刘欢,赵世晶,等.叶酸对小鼠甲基代谢作用的研究[J].营养学报,2014,36(2):145-148.

[33] BROSNAN J T,BROSNAN M E.The sulfur-containing amino acids:an overview[J].The Journal of Nutrition,2006,136(Suppl.6):1636S-1640S.

[34] AISSA A F,TRYNDYAK V,DE CONTI A,et al.Effect of methionine-deficient and methionine-supplemented diets on the hepatic one-carbon and lipid metabolism in mice[J].Molecular Nutrition & Food Research,2014,58(7):1502-1512.

[35] ESPE M,ANDERSEN S M,HOLEN E,et al.Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon[J].British Journal of Nutrition,2014,112(8):1274-1285.

[36] FINKELSTEIN J D.Methionine metabolism in mammals[J].The Journal of Nutritional Biochemistry,1990,1(5):228-237.

[37] PARK E,GARROW L,RENDUCHINTALA M,et al.Methionine deficiency increases betaine-homocysteine methyltransferase (BHMT) activity and mRNA levels in rat liver[J].FASEB Journal,1997,11(3):2121-2121.

[38] PARK E I,GARROW T A.Interaction between dietary methionine and methyl donor intake on rat liver betaine-homocysteine methyltransferase gene expression and organization of the human gene[J].Journal of Biological Chemistry,1999,274(12):7816-7824.

[39] LAMBERT B D,TITGEMEYER E C,STOKKA G L,et al.Methionine supply to growing steers affects hepatic activities of methionine synthase and betaine-homocysteine methyltransferase,but not cystathionine synthase[J].The Journal of Nutrition,2002,132(7):2004-2009.

[40] BARAK A J,TUMA D J.Betaine,metabolic by-product or vital methylating agent?[J].Life Sciences,1983,32(7):771-774.

[41] FINKELSTEIN J D,HARRIS B J,MARTIN J J,et al.Regulation of hepatic betaine-homocysteine methyltransferase by dietary methionine[J].Biochemical and Biophysical Research Communications,1983,108(1):344-348.

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