Effects of Short-Term Feed Restriction on Lipid Metabolism in Liver and Skeletal Muscle of Rabbits

ZHANG Bin, LI Fuchang, WANG Cheng, LIU Hanzhong, YU Zhiju, LIU Lei

doi:10.3969/j.issn.1006-267x.2019.01.030

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 1: 243 - 250

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.01.030

Molecular Nutrition

Effects of Short-Term Feed Restriction on Lipid Metabolism in Liver and Skeletal Muscle of Rabbits

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1. Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, China;
2. Shandong Jianyuan Science and Technology Co., Ltd., Tai'an 271000, China;
3. Sichuan Academy of Grassland Sciences, Chengdu 610091, China

Received date: 2018-06-12

Online published: 2019-01-16

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Abstract

This experiment was conducted to study the effects of short-term feed restriction on expression of lipid metabolism-related genes in liver and skeletal muscle of rabbits, and to expound the regulatory mechanism of lipid metabolism in rabbits under short-term feed restriction. Forty 40-day-old Hyla rabbits with similar weight and good body state were randomly divided into 2 groups (control group and feed restriction group) with 20 replicates (half male and half female) per group and 1 rabbit per replicate. The rabbits in the control group were fed ad libitum, while the rabbits in the feed restriction group were fed with restricted feeding (feeding amount was about 70% of control group). The test period was 5 days. The results showed that short-term feed restriction significantly reduced the liver index of rabbits (P<0.05), and had a decreased tendency in hindleg muscle weight (P=0.074 3), but did not significantly affect the weights of foreleg muscle, dorsal lumbar muscle and total muscle (foreleg muscle+hindleg muscle+dorsal lumbar muscle) (P>0.05). The contents of triglyceride (TG) and very low density lipoprotein (VLDL) in plasma were significantly reduced by short-term feed restriction (P<0.05), but the content of TG in liver and dorsal lumbar muscle was not significantly reduced (P>0.05). In the liver, the mRNA relative expression level of fatty acid synthase (FAS) was significantly down-regulated by short-term feed restriction (P<0.05), and the mRNA relative expression levels of carnitine acyl transferase 1 (CPT1), carnitine acyl transferase 2 (CPT2), G-protein-coupled receptor 41 (GPR41), G-protein-coupled receptor 43 (GPR43) and peroxisome proliferators activate receptor α (PPARα) were significantly up-regulated by short-term feed restriction (P<0.05). In the skeletal muscle, the mRNA relative expression levels of lipoprotein lipase (LPL), fatty acid binding protein (FABP), GPR41 and GPR43 were significantly up-regulated by short-term feed restriction (P<0.05), the mRNA relative expression levels of CPT2 was significantly up-regulated (P<0.05) by short-term feed restriction, and the mRNA relative expression levels of CPT1, PPARα and fatty acid transporters (FATP) were not significantly influenced by short-term feed restriction (P>0.05). In conclusion, short-term feed restriction inhibits lipid synthesis and output, and promotes the oxidation utilization of fatty acids in the liver, and GPR41, GPR43 and PPARα involved in this process; short-term feed restriction promotes the uptake and utilization of fatty acids in skeletal muscles, and GPR41 and GPR43 involved in this process.

Key words： rabbits; short-term feed restriction; lipid metabolism; gene expression; liver; skeletal muscle

Cite this article

ZHANG Bin, LI Fuchang, WANG Cheng, LIU Hanzhong, YU Zhiju, LIU Lei . Effects of Short-Term Feed Restriction on Lipid Metabolism in Liver and Skeletal Muscle of Rabbits[J]. Chinese Journal of Animal Nutrition, 2019 , 31(1) : 243 -250 . DOI: 10.3969/j.issn.1006-267x.2019.01.030

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