Molecular Nutrition

Effects of Plant Essential Oil on Rumen Fermentation Parameters and Methane Production of Mutton Sheep in Vitro

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  • Animal Science and Technology College, Beijing University of Agricultural, Beijing Key Laboratory of Dairy Cow Nutrition, Beijing 102206, China

Received date: 2018-06-13

  Online published: 2019-01-16

Abstract

The objective of this experiment was to study the effects of four kinds of plant essential oils (eucalyptus oil, litsea cubeba oil, cinnamon oil and anise oil)on rumen fermentation parameters and methane (CH4) production of mutton sheep in vitro. The ratio of concentrate to forage of the cultivation substrates was 60:40, and 0 (control), 50, 100, 200 and 400 mg/L of eucalyptus oil, litsea cubeba oil, cinnamon oil or anise oil were added, respectively. There were 3 replicates for each concentration of four plant essential oils. The 24 h gas production, CH4 proportion in gas, pH, volatile fatty acid (VFA) and ammonia nitrogen (NH3-N) concentrations were measured for 24 h simulating rumen fermentation cultivation in vitro. The results showed as follows:1) adding litsea cubeba oil and anise oil significantly affected the rumen fermentation fluid pH in vitro (P<0.05). 2) Compared with the control group, adding 400 mg/L litsea cubeba oil and anise oil significantly decreased the total VFA concentration (P<0.05), and showed a linearly decreased trend with the concentrations of litsea cubeba oil and anise oil increased (P<0.05); adding 400 mg/L litsea cubeba oil and anise oil significantly increased the acetic acid proportion (P<0.05), and showed a firstly decreased and then increased quadratically trend with the concentration of litsea cubeba oil and anise oil increased (P<0.05); adding 200 mg/L anise oil and 400 mg/L eucalyptus oil significantly increased the propionic acid proportion (P<0.05), adding 400 mg/L litsea cubeba oil significantly decreased the propionic acid proportion (P<0.05), and showed a quadratic curve change trend with the concentrations of eucalyptus oil, litsea cubeba oil and anise oil increased (P<0.05); adding 400 mg/L eucalyptus oil and 200 mg/L anise oil significantly decreased the ratio of acetic acid to propionic acid (P<0.05), adding 400 mg/L litsea cubeba oil significantly increased the ratio of acetic acid to propionic acid (P<0.05), and showed a quadratic curve change trend with the concentrations of eucalyptus oil, anise oil and litsea cubeba oil increased (P<0.05). 3) Compared with the control group, adding 400 mg/L litsea cubeba oil and anise oil significantly decreased the NH3-N concentration (P<0.05), and showed a linearly decreased trend with the concentration of anise oil increased (P<0.05). 4) Compared with the control group, adding 400 mg/L litsea cubeba oil and anise oil significantly decreased the gas production (P<0.05), and showed a firstly increased and then decreased quadratically trend with the concentration of litsea cubeba oil increased (P<0.05), and showed a linearly decreased trend with the concentration of anise oil increased (P<0.05); adding 400 mg/L litsea cubeba oil and anise oil significantly decreased the CH4 production (P<0.05), and showed a firstly increased and then decreased quadratically trend with the with the concentration increase of litsea cubeba oil increased (P<0.05), and showed a linearly decreased trend with the concentration of anise oil increased. (P<0.05). The results indicate that different plant essential oils have different effects on rumen fermentation parameters and CH4 production in vitro, and which are related to the concentration of essential oils.

Cite this article

SHI Ning, JIA Miao, LI Yanling . Effects of Plant Essential Oil on Rumen Fermentation Parameters and Methane Production of Mutton Sheep in Vitro[J]. Chinese Journal of Animal Nutrition, 2019 , 31(1) : 274 -284 . DOI: 10.3969/j.issn.1006-267x.2019.01.034

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