Anti-Inflammatory Effects of Different Concentrations of Ethanol Elutions of Allium mongolicum Regel Flavonoids on Lipopolysaccharide-Induced Mouse Peritoneal Macrophage

WANG Cuifang, WANG Terigele, DAN Ni, DU Hongxi, ZHANG Xiuyuan, SA Ruli, AO Changjin

doi:10.3969/j.issn.1006-267x.2019.01.041

2019 , Vol. 31 >Issue 1: 342 - 350

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.01.041

Molecular Nutrition

Anti-Inflammatory Effects of Different Concentrations of Ethanol Elutions of Allium mongolicum Regel Flavonoids on Lipopolysaccharide-Induced Mouse Peritoneal Macrophage

Expand

College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2018-06-25

Online published: 2019-01-16

Fold

Abstract

This experiment was conducted to explore the anti-inflammatory effects of 35% and 75% ethanol elutions of Allium mongolicum Regel flavonoids (AMF) on lipopolysaccharide (LPS)-induced mouse peritoneal macrophage (MPM). The effects of different concentrations (25, 50, 100, 200 and 400 μg/mL) of 35% and 75% ethanol elutions of AMF on cell viability of MPM were examined by cell counting kit-8 assay. The blank control group, LPS stress model group, different concentration ethanol elutions of AMF groups (supplemented with 25, 50 and 100 μg/mL 35% or 75% ethanol elutions of AMF, respectively) were set up, and the effects different concentrations of ethanol elutions of AMF on cytokines and inflammatory factors of MPM were determined using Griess, real time PCR and enzyme-linked immune sorbent assay methods. The result showed that compared with the blank control group, supplemented with 25 to 100 μg/mL 35% and 75% ethanol elutions of AMF could significantly increase the cell viability of MPM (P<0.01). Compared with the LPS stress model group, 35% and 75% ethanol elutions of AMF could significantly decrease the nitric oxide content and inducible nitric oxide synthase mRNA relative expression in MPM (P<0.01), and showed a dose-dependent effect, and the effect of 75% ethanol elutions of AMF was better than 35% ethanol elutions of AMF; 35% ethanol elutions of AMF could significantly decrease the contents of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in MPM (P<0.01), and also could significantly decrease the TNF-α and IL-6 mRNA relative expression (P<0.01); 75% ethanol elutions of AMF could significantly decrease the contents of TNF-α in MPM (P<0.05 or P<0.01), and also could significantly decrease the TNF-α and IL-6 mRNA relative expression (P<0.01). In conclusion, 35% and 75% ethanol elutions of AMF can exert its anti-inflammatory effect via improving cell viability of MPM and affecting secretion of cytokines and inflammatory factors.

Key words： Allium mongolicum Regel; flavonoids; LPS; mouse; peritoneal macrophage; inflammation

Cite this article

WANG Cuifang, WANG Terigele, DAN Ni, DU Hongxi, ZHANG Xiuyuan, SA Ruli, AO Changjin . Anti-Inflammatory Effects of Different Concentrations of Ethanol Elutions of Allium mongolicum Regel Flavonoids on Lipopolysaccharide-Induced Mouse Peritoneal Macrophage[J]. Chinese Journal of Animal Nutrition, 2019 , 31(1) : 342 -350 . DOI: 10.3969/j.issn.1006-267x.2019.01.041

References

[1] 陈海松,刘维俊.抗内毒素药物的研究进展[J].国外医药(抗生素分册),2002,23(4):174-178.

[2] NIJVELDT R J,VAN NOOD E,VAN HOORN D E C,et al.Flavonoids:a review of probable mechanisms of action and potential applications[J].American Journal of Clinical Nutrition,2001,74(4):418-425.

[3] KIM H P,SON K H,CHANG H W,et al.Anti-inflammatory plant flavonoids and cellular action mechanisms[J].Journal of Pharmacological Sciences,2004,96(3):229-245.

[4] 杨杰,沙金丹,高翔,等.黄酮类化合物的免疫调节作用及机制[J].动物营养学报,2017,29(12):4295-4300.

[5] 中国科学院中国植物志编辑委员会.中国植物志(14卷)[M].北京:科学出版社,1980:170-172.

[6] 敖长金.沙葱化学成分及其生物学功能研究进展[J].饲料工业,2010,31(18):1-5.

[7] 萨茹丽.沙葱黄酮提取工艺优化、结构鉴定及其相关生物活性研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2014.

[8] 木其尔.沙葱黄酮对肉羊机体抗氧化和免疫功能的影响及其机理研究[D].博士学位论文.呼和浩特:内蒙古农业大学,2016.

[9] 陈仁伟.沙葱黄酮对肉羊生产性能及其肉品质的影响[D].硕士学位论文.呼和浩特:内蒙古农业大学,2016.

[10] RAY A,DITTEL B N.Isolation of mouse peritoneal cavity cells[J].Journal of Visualized Experiments,2010(35):1488.

[11] LIU B,ZHANG N S,LIU Z C,et al.RP105 involved in activation of mouse macrophages via TLR2 and TLR4 signaling[J].Molecular and Cellular Biochemistry,2013,378(1/2):183-193.

[12] 刘博.小鼠巨噬细胞TLR2、TLR4及RP105在金黄色葡萄球菌感染中的天然免疫应答机制[D].博士学位论文.长春:吉林大学,2013.

[13] SONG M,CHEN T,PROUGH R A,et al.Chronic alcohol consumption causes liver injury in high-fructose-fed male mice through enhanced hepatic inflammatory response[J].Alcoholism Clinical & Experimental Research,2016,40(3):518-528.

[14] GUO D,LI J R,WANG Y,et al.Cyclovirobuxinum D suppresses lipopolysaccharide-induced inflammatory responses in murine macrophages in vitro by blocking JAK-STAT signaling pathway[J].Acta Pharmacologica Sinica,2014,35(6):770-778.

[15] PARK J W,KWON O K,JANG H Y,et al.A leaf methanolic extract of Wercklea insignis attenuates the lipopolysaccharide-induced inflammatory response by blocking the NF-κB signaling pathway in RAW 264.7 macrophages[J].Inflammation,2012,35(1):321-331.

[16] ZHANG X,SUN C Y,ZHANG Y B,et al.Kegan Liyan oral liquid ameliorates lipopolysaccharide-induced acute lung injury through inhibition of TLR4-mediated NF-κB signaling pathway and MMP-9 expression[J].Journal of Ethnopharmacology,2016,186:91-102.

[17] GORDON S,TAYLOR P R.Monocyte and macrophage heterogeneity[J].Nature Reviews Immunology,2005,5(12):953-964.

[18] SOARES J B,PIMENTEL-NUNES P,RONCON-ALBUQUERQUE R Jr.,et al.The role of lipopolysaccharide/Toll-like receptor 4 signaling in chronic liver diseases[J].Hepatology International,2010,4(4):659-672.

[19] AN H J,JEONG H J,UM J Y,et al.Glechoma hederacea inhibits inflammatory mediator release in IFN-γ and LPS-stimulated mouse peritoneal macrophages[J].Journal of Ethnopharmacology,2006,106(3):418-424.

[20] MÖLLER B,VILLIGER P M.Inhibition of IL-1,IL-6,and TNF-α in immune-mediated inflammatory diseases[J].Springer Seminars in Immunopathology,2006,27(4):391-408.

[21] LEGRAND A,FERMOR B,FINK C,et al.Interleukin-1,tumor necrosis factor α,and interleukin-17 synergistically up-regulate nitric oxide and prostaglandin E₂ production in explants of human osteoarthritic knee menisci[J].Arthritis & Rheumatology,2001,44(9):2078-2083.

[22] NAM T G,LIM T G,LEE B H,et al.Comparison of anti-inflammatory effects of flavonoid-rich common and tartary buckwheat sprout extracts in lipopolysaccharide-stimulated RAW 264.7 and peritoneal macrophages[J].Oxidative Medicine and Cellular Longevity,2017,2017:9658030.

[23] JOH E H,KIM D H.Lancemaside A inhibits lipopolysaccharide-induced inflammation by targeting LPS/TLR4 complex[J].Journal of Cellular Biochemistry,2010,111(4):865-871.

[24] FAN G W,JIANG X R,WU X Y,et al.Anti-inflammatory activity of tanshinone ⅡA in LPS-stimulated RAW264.7 macrophages via miRNAs and TLR4-NF-κB pathway[J].Inflammation,2016,39(1):375-384.

[25] XU X,YIN P,WAN C,et al.Punicalagin inhibits inflammation in LPS-induced RAW264.7 macrophages via the suppression of TLR4-mediated MAPKs and NF-κB activation[J].Inflammation,2014,37(3):956-965.

[26] LIAO Y R,LIN J Y.Quercetin,but not its metabolite quercetin-3-glucuronide,exerts prophylactic immunostimulatory activity and therapeutic antiinflammatory effects on lipopolysaccharide-treated mouse peritoneal macrophages ex vivo[J].Journal of Agricultural and Food Chemistry,2014,62(13):2872-2880.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References