Physiological Effects of Earthworm Extracts in Mice

LIU Hanyang, WU Yongsheng, ZHU Jiawen, CHEN Yaying, XU Qilin, XU Zhenying, YANG Xue, QIU Shixiu, QI Guilan, LI Juan, SUN Yuehong, LEI Chunlong

doi:10.3969/j.issn.1006-267x.2019.01.051

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 1: 437 - 443

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.01.051

Molecular Nutrition

Physiological Effects of Earthworm Extracts in Mice

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Institute of Animal Science, Chengdu Academy of Agriculture and Forestry Sciences, Chengdu 611130, China

Received date: 2018-06-26

Online published: 2019-01-16

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Abstract

This study was conducted to investigate the effects of earthworm extracts on growth performance, serum biochemical indexes, antioxidant indexes and immune indexes in mice. A total of 128 mice at 21 days of age were randomly allotted to 4 groups with 8 replicates per group and 4 mice per replicate. The mice in control group were fed a basal diet, and those in 4 experimental groups (groups Ⅰ, Ⅱ and Ⅲ) were fed the basal diet added earthworm extracts at concentrations (diet/earthworm liquid) of 1 g/5 μL, 1 g/10 μL and 1 g/15 μL, respectively. The experiment lasted for 28 days. The results showed as follows:1) there was no significant difference in average daily feed intake and average daily gain among groups (P>0.05); the feed to grain ratio of group Ⅰ was significantly lower than that of other groups (P<0.05),and it was decreased by 8.47% compared with the control group (P<0.05). The heart index of groups Ⅰ, Ⅱ and Ⅲ was significantly higher than that of the control group (P<0.05). 2) There was no significant difference in serum glucose (GLU), total protein (TP) and urea (UN) contents among groups (P>0.05). 3)The activity of superoxide dismutase (SOD) in serum of group Ⅰ was significantly higher than that of the control group (P<0.05). The total antioxidant capacity of groups Ⅰ and Ⅱ was significantly higher than that of control group and group Ⅲ (P<0.05). 4) The spleen index of group Ⅰ was significantly higher than that of group Ⅱ and control group (P<0.05). The serum immune globulin G(IgG), interferon-γ (IFN-γ) and interleukin-6 (IL-6) contents of group Ⅰ were significantly higher than those of the control group (P<0.05). The serum lysozyme activity and tumour necrosis factor-α (TNF-α) contents of groups Ⅰ, Ⅱ and Ⅲ were significantly higher than those of the control group (P<0.05). In summary, under the conditions of this experiment, earthworm extracts have functions of promoting the development of growth performance and immune organs and regulating serum immune, as well as antioxidant indexes of mice. Earthworm extracts have a potential development value as a feed additive.

Key words： earthworm extracts; growth performance; antioxidant capacity; immune index

Cite this article

LIU Hanyang, WU Yongsheng, ZHU Jiawen, CHEN Yaying, XU Qilin, XU Zhenying, YANG Xue, QIU Shixiu, QI Guilan, LI Juan, SUN Yuehong, LEI Chunlong . Physiological Effects of Earthworm Extracts in Mice[J]. Chinese Journal of Animal Nutrition, 2019 , 31(1) : 437 -443 . DOI: 10.3969/j.issn.1006-267x.2019.01.051

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