Alleviation Effects of Tea Polyphenol on Combined Exposure to Heavy Metal in Laying Hens

WANG Jianping, YANG Zengqiao, DING Xuemei, BAI Shiping, ZENG Qiufeng, ZHANG Keying

doi:10.3969/j.issn.1006-267x.2019.01.052

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 1: 444 - 451

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.01.052

Molecular Nutrition

Alleviation Effects of Tea Polyphenol on Combined Exposure to Heavy Metal in Laying Hens

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Animal Nutrition Institute, Key Laboratory for Animal Disease-Resistance Nutrition of China, Ministry of Education, Key Laboratory for Animal Disease-Resistance Nutrition and Feed of China, Ministry of Agriculture, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2018-06-04

Online published: 2019-01-16

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Abstract

The objective of this experiment was to study the effects of dietary exposure of Pb, Cd, V and Mo on the performance, egg quality, heavy metal retention and serum antioxidant status in laying hens and the alleviation effects of tea polyphenol (TP). A total of 300 Pink-eggshell Lohnman laying hens (65-wk-old) were randomly allotted into 3 groups (10 replicates/group, 10 birds/replicate), including control (CON) group (basal diet), combined exposure to heavy metal (HEM) group (CON group+5 mg/kg Pb+0.5 mg/kg Cd+5 mg/kg V+10 mg/kg Mo) and TP group (HEM group + 1 000 mg/kg TP). This experiment lasted for 5 weeks. The results showed as follows:1) compared with the CON group, the daily egg production ratio in the HEM group significantly decreased by 8.26% (P<0.05) and feed to egg ratio significantly increased by 16.06% (P<0.05) and the soft eggshell egg ratio significantly increased (P<0.05). The daily egg production ratio and feed to egg ratio in TP group had no significant difference compared with the CON group (P>0.05). 2) Compared with the CON group, the eggshell thickness and eggshell color significantly decreased (P<0.05), lightness value significantly increased (P<0.05), redness and yellowness values significantly decreased (P<0.05), the albumen height and Haugh unit were also decreased by 20.08% and 16.19% (P<0.05). The eggshell thickness and eggshell color were significantly lower than those in CON group (P<0.05), albumen height and Haugh unit were significantly higher than those in HEM group (P<0.05), but had no significant difference compared with the CON group (P>0.05). 3) Compared with the CON group, the Fe, Zn and Se contents in egg in HEM group significantly decreased by 23.12%, 28.66%, 65.43% (P<0.05), and the Pb, Cd, V and Mo contents in egg significantly increased by 30.42%, 21.58%, 51.67% and 30.66% (P<0.05), respectively. The contents of microelement and heavy metal had the same trend with those in HEM group, but the contents of Cd, Pb, V, Mo in eggs were not decreased. 4) Compared with the CON group, serum glutathione peroxidase (GSH-Px) activity and glutathione (GSH) content in HEM group significantly decreased by 25.37% and 53.97% (P<0.05), and MDA content significantly increased by 100.00% (P<0.05). Compared with HEM group, GSH-Px activity and GSH content in TP group significantly increased (P<0.05). In conclusion, dietary heavy metal exposure lead to reduction in performance and egg quality, can increase the heavy metal retention in egg, and lower antioxidative status, while TP can alleviate the effects of heavy metal on performance and egg quality, which may attribute to its antioxidaitve function in laying hens.

Key words： heavy metal; tea polyphenols; laying hens; performance; egg quality

Cite this article

WANG Jianping, YANG Zengqiao, DING Xuemei, BAI Shiping, ZENG Qiufeng, ZHANG Keying . Alleviation Effects of Tea Polyphenol on Combined Exposure to Heavy Metal in Laying Hens[J]. Chinese Journal of Animal Nutrition, 2019 , 31(1) : 444 -451 . DOI: 10.3969/j.issn.1006-267x.2019.01.052

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