Effects of Folic Acid and Vitamin B12 on Liver Lipid Phosphate Phosphohydrolase 1 Gene Expression and Its Correlation with Serum Lipid Metabolism, Fat Deposition and Meat Quality Indices of Wulong Geese

CHENG Manman, WANG Baowei, ZHANG Tingrong, KONG Min, ZHANG Ming, YUE Bin, GE Wenhua

doi:10.3969/j.issn.1006-267x.2019.02.023

2019 , Vol. 31 >Issue 2: 669 - 680

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.02.023

Molecular Nutrition

Effects of Folic Acid and Vitamin B₁₂ on Liver Lipid Phosphate Phosphohydrolase 1 Gene Expression and Its Correlation with Serum Lipid Metabolism, Fat Deposition and Meat Quality Indices of Wulong Geese

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Nutrition and Feed Laboratory of China Agriculture Research System, Institute of High Quality Waterfowl, Qingdao Agricultural University, Qingdao 266109, China

Received date: 2018-07-02

Online published: 2019-02-18

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Abstract

This study was conducted to investigated the effect of dietary different supplemental levels of folic acid and vitamin B₁₂ on liver lipid phosphate phosphohydrolase 1 (Lpin1) gene expression of Wulong geese and its expression difference in different tissues, and to further analysis the correlation of Lpin1 gene expression with serum lipid metabolism, fat deposition and meat quality indices. A total of 420 five-week-old Wulong geese were randomly divided into 7 groups with 6 replicates per group and 10 geese per replicate. The trial used a 2×3 two factors (folic acid supplemental level×vitamin B₁₂ supplemental level) across-equal factorial design, geese in the blank control group were fed a basal diet without folic acid and vitamin B₁₂; geese in experimental groups were fed experiment diets, which folic acid supplemental levels were 0.25, 2.00 mg/kg, respectively, and vitamin B₁₂ supplemental levels were 0.003, 0.009, 0.018 mg/kg, respectively. The experiment lasted for 4 weeks. The results showed as follow:1) the interaction of different supplemental levels of folic acid and vitamin B₁₂ had significant effect on the final weight of Wulong geese (P<0.05). 2) The interaction of different supplemental levels of folic acid and vitamin B₁₂ had significant effects on the percentage of subcutaneous fat, percentage of abdominal fat, intermuscular fat bandwidth, intramuscular fat rate of breast muscle, intramuscular fat rate of leg muscle of Wulong geese (P<0.05). 3) Different supplemental levels of folic acid and vitamin B₁₂ and their interaction had significant effect on the liver Lpin1 gene expression of Wulong geese (P<0.05). 4) The Lpin1 gene expression was detected in 11 tissues of heart, liver, kidney, abdominal fat, lung, gizzard, glandular stomach, breast muscle, leg muscle, spleen and pancreas; the Lpin1 gene expression in abdominal fat was the highest, and it significantly higher than that in other tissues (P<0.05); secondly was in gizzard, pancreas and lung, significantly higher than that in glandular stomach, heart, kidney, leg muscle and breast muscle (P<0.05). 5) The liver Lpin1 gene expression of Wulong geese had significant negative correlations with the contents of triglyceride and high density lipoprotein cholesterol in serum (P<0.05), and had negative correlations with the contents of glucose, total cholesterol, low density lipoprotein cholesterol in serum (P>0.05). 6) The liver Lpin1 gene expression of Wulong geese had significant positive correlations with the percentage of abdominal fat and intramuscular fat rate of leg muscle (P<0.05), had significant positive correlations with the intermuscular fat bandwidth, intramuscular fat rate of breast muscle (P<0.01), and had a negative correlation with the percentage of subcutaneous fat (P>0.05). 7) The liver Lpin1 gene expression of Wulong geese had no significant correlation with the pH, meat color, shearing force and water loss rate in breast muscle and leg muscle (P>0.05), but had a significant positive correlation with the drip loss of leg muscle (P<0.05). In conclusion, different supplemental levels of folic acid and vitamin B₁₂ and their interaction have significant effects on the liver Lpin1 gene expression of Wulong geese, and the Lpin1 gene expression has a certain correlation with serum lipid metabolism, fat deposition and meat quality indices.

Key words： folic acid; vitamin B₁₂; Wulong geese; lipid phosphate phosphohydrolase 1; lipid metabolism; meat quality

Cite this article

CHENG Manman, WANG Baowei, ZHANG Tingrong, KONG Min, ZHANG Ming, YUE Bin, GE Wenhua . Effects of Folic Acid and Vitamin B₁₂ on Liver Lipid Phosphate Phosphohydrolase 1 Gene Expression and Its Correlation with Serum Lipid Metabolism, Fat Deposition and Meat Quality Indices of Wulong Geese[J]. Chinese Journal of Animal Nutrition, 2019 , 31(2) : 669 -680 . DOI: 10.3969/j.issn.1006-267x.2019.02.023

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