Changes in Serum Biochemical Indices, Duodenal Morphology, Expression Levels of Sodim-Gluose Transporter 1 and Peptidetransporter 1 Genes in Jejunum of Piglets at the First Week Post-Weaning

LI Mengyun, NIE Furong, CHENG Wei, ZHU Kuanyou, GUO Liangxing, ZHANG Tianyong, LIU Yanhe

doi:10.3969/j.issn.1006-267x.2019.02.032

2019 , Vol. 31 >Issue 2: 757 - 763

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.02.032

Molecular Nutrition

Changes in Serum Biochemical Indices, Duodenal Morphology, Expression Levels of Sodim-Gluose Transporter 1 and Peptidetransporter 1 Genes in Jejunum of Piglets at the First Week Post-Weaning

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1. College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China;
2. Henan Guang An Bio-Tech Co., Ltd, Zhengzhou 450001, China

Received date: 2018-07-06

Online published: 2019-02-18

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Abstract

The experiment was conducted to investigate the changes in serum biochemical indices, duodenal morphology and expression levels of sodim-gluose transporter 1 (SGLT1) and peptidetransporter 1 (PepT1) genes in jejunum of piglets at pre-weaning and the first week post-weaning. A total of 55 piglets at 20 days of age were selected, five piglets were slaughtered at 1 d pre-weaning, and the remained 50 piglets (weaned at 21 days of age) were randomly divided to 5 groups (pens) with 10 piglets per pen. One piglet per pen was sacrificed on d 2, 4 and 8 post-weaning, respectively. The results showed as follows:1) compared with pre-weaning, the alkaline phosphatase activity in serum on d 2 post-weaning was significantly decreased (P<0.05) and was very significantly decreased on d 4 post-weaning (P<0.01); serum potassium ion content was significantly increased on d 4 and 8 post-weaning (P<0.05); serum urea nitrogen content was very significantly increased on d 2 post-weaning (P<0.01) and significantly decreased on d 8 post-weaning (P<0.05); serum immunoglobulin G content was significantly decreased after weaning (P<0.05). 2) Compared with pre-weaning, the duodenal villus height was significantly decreased on d 2 post-weaning (P<0.01)and d 4 post-weaning (P<0.05), crypt depth in duodenum was significantly enhanced on d 2 and 4 post-weaning (P<0.05), and the ratio of villus height to crypt depth was also significantly decreased on d 2 and 4 post-weaning (P<0.05). 3) Compared with pre-weaning, the expression levels of SGLT1 gene in jejunum was also significantly decreased after weaning (P<0.05); the expression level of PepT1 gene in jejunum on d 4 and 8 post-weaning was significantly decreased (P<0.05). These results suggest that weaning on the first week causes a certain stress on physiological functions of piglets. Weaning reduces the serum immunoglobulin G content, changes the duodenal morphology, decreases the expression levels of SGLT1 and PepT1 genes in jejunum, which may affect the digestion and absorption of carbohydrates and proteins, especially on d 2 and 4 post-weaning.

Key words： piglets; serum biochemical indices; intestinal morphology; gene expression; pre-and post-weaning

Cite this article

LI Mengyun, NIE Furong, CHENG Wei, ZHU Kuanyou, GUO Liangxing, ZHANG Tianyong, LIU Yanhe . Changes in Serum Biochemical Indices, Duodenal Morphology, Expression Levels of Sodim-Gluose Transporter 1 and Peptidetransporter 1 Genes in Jejunum of Piglets at the First Week Post-Weaning[J]. Chinese Journal of Animal Nutrition, 2019 , 31(2) : 757 -763 . DOI: 10.3969/j.issn.1006-267x.2019.02.032

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