Molecular Nutrition

Polysaccharide of Atractylodes Macrocephala Koidz May Alleviates Liver Injury Induced by Cyclophosphamide Via Toll-Like Receptor 4 Signaling Pathway in Goslings

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  • 1. Zhongkai University of Agricultural and Engineering, Guangzhou 510225, China;
    2. Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou 510225, China

Received date: 2018-07-13

  Online published: 2019-02-18

Abstract

This trial was conducted to evaluate the effects and possible mechanism of polysaccharide of Atractylodes macrocephala Koidz (PAMK) on liver injury induced by cyclophosphamide (CTX) in goslings. A total of 180 healthy one-day old Magang goslings were randomly divided into 3 groups (control group, CTX group and PAMK+CTX group) with 3 replicates per group and 20 geese per replicate in a single-factor completely randomized experimental design. Goslings in PAMK+CTX group were fed the basal diet supplemented with 400 mg/kg PAMK from 4 days of age to the end of the trial. On 19, 20 and 21 days of age, goslings in the control group were intramuscular injected 0.5 mL normal saline, and goslings in CTX group and PAMK+CTX group were intramuscular injected 40 mg/kg BW CTX solution, respectively. The trail lasted for 28 days. The results showed as follows:1) compared with the control group, the liver index of control group was significantly increased (P<0.05), and the gallbladder index was significantly decreased (P<0.05). The liver index and gallbladder index of PAMK+CTX group had no significant difference compared with the control group (P>0.05). 2) The liver 8-hydroxy-deoxyguanosine content of CTX group and PAMK+CTX group was significantly higher than that of the control group (P<0.05). 3) The CTX could cause liver pathological damages, DNA oxidative damage of hepatic cells and some lesions in the liver, such as tissue fibrosis, inflammatory small focus, cholestasis et al. The PAMK could alleviate extent of damage of liver tissue, reduce the number of necrotic hepatocytes. 4) Compared with the control group, the liver malondialdehyde (MDA) content of CTX group was significantly increased (P<0.05), and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), inducible nitric oxide synthase (iNOS) in liver were significantly decreased (P<0.05). The MDA content and GSH-Px, iNOS activities in liver of PAMK+CTX group had no significant difference compared with the control group (P>0.05). 5) The mRNA relative expressions of Toll-like receptor 4 (TLR4), tumor necrosis factor receptor-associated factor 6 (TRAF6), mitogen activated protein kinase kinase 1 (MEKK1), mitogen-activated protein kinase kinase 3 (MKK3), p38 mitogen-activated protein kinase (p38MAPK), inhibitor of nuclear factor-κB α (IκBα) and inhibitor of nuclear factor kappa-B kinase α (IKKα) in liver of CTX group were significantly lower than those of the control group (P<0.05). The mRNA relative expressions of TLR4, myeloid differentiation primary response (MyD88), MEKK1, MKK3, mitogen-activated protein kinase kinase 6 (MKK6), inhibitor of nuclear factor kappa-B kinase ε (IKKε) and IκBαin liver of PAMK+CTX group were significantly higher than those of CTX group (P<0.05), the mRNA relative expressions of MyD88 and MKK6 in liver were significantly higher than those of the control group (P<0.05), and the mRNA relative expressions of TRAF6, MEKK1, MKK3 and p38MAPK in liver were significantly lower than those of the control group (P<0.05). These results suggested that PAMK may regulate liver injury induced by CTX in goslings via TLR4 signaling pathway.

Cite this article

QIAN Long, LIU Yang, LI Bingxin, FU Jing, LI Wanyan, CAO Nan, TIAN Yunbo, XU Danning . Polysaccharide of Atractylodes Macrocephala Koidz May Alleviates Liver Injury Induced by Cyclophosphamide Via Toll-Like Receptor 4 Signaling Pathway in Goslings[J]. Chinese Journal of Animal Nutrition, 2019 , 31(2) : 764 -774 . DOI: 10.3969/j.issn.1006-267x.2019.02.033

References

[1] 鲍轶.免疫细胞在非酒精性脂肪性肝病中的作用机制及研究进展[J].中华肝脏病杂志,2017,25(7):553-556.

[2] XI Q Y,JIANG Y,ZHAO S,et al.Effect of ginseng polysaccharides on the immunity and growth of piglets by dietary supplementation during late pregnancy and lactating sows[J].Animal Science Journal,2017,88(6):863-872.  

[3] ZHU N,LV X C,WANG Y Y,et al.Comparison of immunoregulatory effects of polysaccharides from three natural herbs and cellular uptake in dendritic cells[J].International Journal of Biological Macromolecules,2016,93:940-951.

[4] SUN W J,MENG K,QI C H,et al.Immune-enhancing activity of polysaccharides isolated from Atractylodis macrocephalae Koidz[J].Carbohydrate Polymers,2015,126:91-96.

[5] XU D N,LI W Y,HUANG Y M,et al.The effect of selenium and polysaccharide of Atractylodes macrocephala Koidz. (PAMK) on immune response in chicken spleen under heat stress[J].Biological Trace Element Research,2014,160(2):232-237.  

[6] XU D N,TIAN Y B.Selenium and polysaccharides of Atractylodes macrocephala Koidz play different roles in improving the immune response induced by heat stress in chickens[J].Biological Trace Element Research,2015,168(1):235-241.  

[7] XU D N,LI W Y,LI B X,et al.The effect of selenium and polysaccharide of Atractylodes macrocephala Koidz. (PAMK) on endoplasmic reticulum stress and apoptosis in chicken spleen induced by heat stress[J].RSC Advances,2017,7(13):7519-7525.  

[8] XU D N,LI B X,CAO N,et al.The protective effects of polysaccharide of Atractylodes macrocephala Koidz (PAMK) on the chicken spleen under heat stress via antagonizing apoptosis and restoring the immune function[J].Oncotarget,2017,8(41):70394-70405.

[9] MOIGNET A,HASANALI Z,ZAMBELLO R,et al.Cyclophosphamide as a first-line therapy in LGL leukemia[J].Leukemia,2014,28(5):1134-1136.  

[10] HABIBI E,SHOKRZADEH M,CHABRA A,et al.Protective effects of Origanum vulgare ethanol extract against cyclophosphamide-induced liver toxicity in mice[J].Pharmaceutical Biology,2015,53(1):10-15.  

[11] MANSOUR D F,SALEH D O,MOSTAFA R E.Genistein ameliorates cyclophosphamide-induced hepatotoxicity by modulation of oxidative stress and inflammatory mediators[J].Open Access Macedonian Journal of Medical Sciences,2017,5(7):836-843.  

[12] ZHOU Y L,CHEN X Y,YI R K,et al.Immunomodulatory effect of tremella polysaccharides against cyclophosphamide-induced immunosuppression in mice[J].Molecules,2018,23(2):E239.

[13] LI Q,CHEN G Y,CHEN H,et al.Se-enriched G. frondosa polysaccharide protects against immunosuppression in cyclophosphamide-induced mice via MAPKs signal transduction pathway[J].Carbohydrate Polymers,2018,196:445-456.

[14] LIN S T,HAO G X,LONG M,et al.Oyster (Ostrea plicatula Gmelin) polysaccharides intervention ameliorates cyclophosphamide-induced genotoxicity and hepatotoxicity in mice via the Nrf2-ARE pathway[J].Biomedicine & Pharmacotherapy,2017,95:1067-1071.

[15] SZABO G,DOLGANIUC A,MANDREKAR P.Pattern recognition receptors:a contemporary view on liver diseases[J].Hepatology,2006,44(2):287-298.  

[16] GUSTOT T,LEMMERS A,MORENO C,et al.Differential liver sensitization to toll-like receptor pathways in mice with alcoholic fatty liver[J].Hepatology,2006,43(5):989-1000.  

[17] STÄRKEL P,DE SAEGER C,STRAIN A J,et al.NFκB,cytokines,TLR 3 and 7 expression in human end-stage HCV and alcoholic liver disease[J].European Journal of Clinical Investigation,2010,40(7):575-584.  

[18] SCHWABE R F,SEKI E,BRENNER D A.Toll-like receptor signaling in the liver[J].Gastroenterology,2006,130(6):1886-1900.  

[19] JI G Q,CHEN R Q,ZHENG J X.Macrophage activation by polysaccharides from Atractylodes macrocephala Koidz through the nuclear factor-κB pathway[J].Pharmaceutical Biology,2015,53(4):512-517.  

[20] YOON Y D,HAN S B,KANG J S,et al.Toll-like receptor 4-dependent activation of macrophages by polysaccharide isolated from the radix of Platycodon grandiflorum[J].International Immunopharmacology,2003,3(13/14):1873-1882.

[21] KIM S J,PARK H J,SHIN H J,et al.Suppression of TRIF-dependent signaling pathway of toll-like receptors by allyl isothiocyanate in RAW 264.7 macrophages[J].International Immunopharmacology,2012,13(4):403-407.  

[22] ZHOU L J,LIU Z J,WANG Z X,et al.Astragalus polysaccharides exerts immunomodulatory effects via TLR4-mediated MyD88-dependent signaling pathway in vitro and in vivo[J].Scientific Reports,2017,7:44822.

[23] BORAZJANI N J,TABARSA M,YOU S G,et al.Purification,molecular properties,structural characterization,and immunomodulatory activities of water soluble polysaccharides from Sargassum angustifolium[J].International Journal of Biological Macromolecules,2018,109:793-802.
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