Differentially Expression of Whey Protein in Different Mammary Glands of Dairy Cows

WANG Yue, XIONG Benhai, JIANG Linshu

doi:10.3969/j.issn.1006-267x.2019.02.034

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 2: 775 - 791

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.02.034

Molecular Nutrition

Differentially Expression of Whey Protein in Different Mammary Glands of Dairy Cows

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1. Key Laboratory of Dairy Nutrition in Beijing, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
2. Beijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2018-07-12

Online published: 2019-02-18

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Abstract

The objective of this study was to explore the effects of dairy breast health conditions on milk proteins and the molecular mechanisms of inflammation induced by pathogenic microorganisms by investigating the differential expression of whey proteins in dairy cows under different breast health status, which provided a potential biomarker to diagnose the early inflammation of udder. A total of 102 healthy Holstein dairy cows[2 to 3 parity, lactation days (152±27) d, milk yield (27±3) kg/d] were selected for milk sample collection. Milk samples were divided into 6 groups according to somatic cell count (SCC) and bacteriological identification results, they were bacterial culture-positive groups (contagious, environmental and opportunistic) and bacterial culture-negative groups[culture-negative-low SCC group (SCC<100 000 cells/mL), culture-negative-medium SCC group (SCC 100 000 to 400 000 cells/mL) and culture-negative-high SCC group (SCC>400 000 cells/mL)]. The sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE) and non-labeled quantitative proteomics were used to identify protein function and expression levels in 6 whey samples. The results showed as follows:1) the difference between healthy breast and inflammatory mammary glands in dairy cows was mainly caused by the protein expression level. 2) A total of 272 proteins were identified, of which 58 proteins showed significant regulatory changes. Compared with bacterial culture-negative whey samples, 20 proteins were significantly up-regulated expressed in bacterial culture-positive whey samples, and the expressions of cathelicidin-4 (CATHL4), cathelicidin-3(CATHL3), cathelicidin-2(CATHL2), inter-alpha-trypsin inhibitor heavy chain H4, (ITIH4), serpin A3-1 (SERPINA3-1), prostaglandin-H2 D-isomerase (PTGDS), serum amyloid A protein (SAA3) and immunoglobulin kappa variable 3-20 (IGKV3-20) in bacterial culture-positive whey samples were more than about 8 times than those in bacterial culture-positive whey samples, and most of the functions of the up-regulated proteins were related to specific immune responses. The 38 proteins significantly down-regulated expressed(P<0.05 or P<0.01), the platelet glycoprotein 4 (CD36), CD59 molecule (CD59), kappa-casein (CSN3), butyrophilin subfamily 1 member A1 (BTN1A1), ATP-binding cassette sub-family G member 2 (ABCG2), perilipin-2 (PLIN2), glycoprotein 2 (GP2), polyubiquitin-C (UBC) and isocitrate dehydrogenase 1 (IDH1) were significantly down-regulated(P<0.01), BTN1A1 was related to the lipid synthesis and secretion, PLIN2 and GP2 were related to the transport, ABCG2 had enzyme activity, CD59 was related to the immune system. 3) A total of 21 bacterial proteins were identified in the bacterial culture-positive whey samples, the existence of these bacterial proteins not only verified the results of bacterial culture identification, but also elucidated the molecules that induced inflammation by the pathogenic microorganisms from the perspective of the bacterial protein properties and functions. In summary, the differentially expression of milk proteins under different breast health conditions can reveals the biological phenomena in the mammary gland under different breast health conditions from the perspective of milk protein expression changes and function, in addition, it expects to provide some potential biomarkers for diagnosing the breast health, especially the early breast inflammation.

Key words： dairy cow; proteomics; SCC; breast health

Cite this article

WANG Yue, XIONG Benhai, JIANG Linshu . Differentially Expression of Whey Protein in Different Mammary Glands of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2019 , 31(2) : 775 -791 . DOI: 10.3969/j.issn.1006-267x.2019.02.034

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