Effects of Curcumin on Growth Performance, Oxidative Stress Resistance and Expression of Nuclear Factor Erythroid 2-Related Factor 2/Antioxidant Responsive Element Signaling Pathway-Related Genes in Grass Carp (Ctenopharyngodon idella)

MING Jianhua, YE Jinyun, ZHANG Yixiang, YANG Xia, SHAO Xianping, QIANG Jun, XU Pao

doi:10.3969/j.issn.1006-267x.2019.02.037

2019 , Vol. 31 >Issue 2: 809 - 823

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.02.037

Molecular Nutrition

Effects of Curcumin on Growth Performance, Oxidative Stress Resistance and Expression of Nuclear Factor Erythroid 2-Related Factor 2/Antioxidant Responsive Element Signaling Pathway-Related Genes in Grass Carp (Ctenopharyngodon idella)

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1. Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, Chinese Academy of Fishery Sciences, College of Life Science, Huzhou University, Huzhou 313000, China;
2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China

Received date: 2018-08-20

Online published: 2019-02-18

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Abstract

The present study aimd to investigate the effects of curcumin on growth performance, oxidative stress resistance and expression of nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant responsive element (ARE) signaling pathway-related genes in grass carp (Ctenopharyngodon idella). Five hundred and twenty-five juvenile grass carp with an initial body weight of (5.11±0.08) g were randomly distributed into five groups with three replicates each, fed five diets supplemented with graded levels of curcumin[0 (control), 200, 400, 600 and 800 mg/kg diet] for 60 days. At the end of the feeding experiment, fifteen fish per tank were injected intraperitoneally with 10 mg diquat/kg body weight (BW) to carry out acute oxidative stress test. The blood and liver of grass carp were sampled before (0 h) and after 24 h of injection, and their physiological and biochemical indicators, as well as the mRNA expression levels of liver antioxidant-related genes were detected. The results showed as follows:1) with the increase of dietary curcumin supplementation, the weight gain rate (WGR), specific growth rate (SGR) and survival rate of juvenile grass carp increased first and then decreased, while the feed conversion ratio (FCR) and hepatosomatic index (HSI) decreased first and then increased; the growth performance of grass carp was better when diets supplemented with 400, 600 mg/kg curcumin. There was no significant difference in viscerosomatic index (VSI) and condition factor (CF) among the experimental groups (P>0.05). 2) Before diquat injection, there was not significantly different in serum cortisol (COR), glucose (GLU) contents, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities among groups (P>0.05). After 24 h of diquat injection, above indicators in serum decreased first and then increased, and which reached the lower levels when dietary curcumin supplementation was 400, 600 mg/kg and were lower than those in the control group (P<0.05). 3) Befor and after 24 h of diquat injection, with the increase of dietary curcumin supplementation, liver superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities, total antioxidant capacity (T-AOC) and glutathione (GSH) content, the mRNA expression levels of Nrf2, copper and zinc superoxide dismutase (Cu-ZnSOD), manganese superoxide dismutase (MnSOD), CAT and GSH-Px and heat shock protein 70 (HSP70) genes increased first and then decreased, while liver reactive oxygen species (ROS) and malondialdehyde (MDA) content, and mRNA expression level of Kelch-like ECH-associated protein1 (Keap1) trended to decrease first and then increase. When diet supplemented with 400, 600 mg/kg curcumin, these indexes reached the higher of lower levels, respectively. Therefore, the diet supplemented with 400, 600 mg/kg curcumin can promote the growth of juvenile grass carp, reduce FCR, improve antioxidative stress ability, and protect fish against acute oxidative stress injury induced by diquat; can activate the Nrf2/ARE signaling pathway of grass carp.

Key words： grass carp (Ctenopharyngodon idella); curcumin; growth performance; oxidative stress resistance; gene expression

Cite this article

MING Jianhua, YE Jinyun, ZHANG Yixiang, YANG Xia, SHAO Xianping, QIANG Jun, XU Pao . Effects of Curcumin on Growth Performance, Oxidative Stress Resistance and Expression of Nuclear Factor Erythroid 2-Related Factor 2/Antioxidant Responsive Element Signaling Pathway-Related Genes in Grass Carp (Ctenopharyngodon idella)[J]. Chinese Journal of Animal Nutrition, 2019 , 31(2) : 809 -823 . DOI: 10.3969/j.issn.1006-267x.2019.02.037

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