Effects of Single and Combined Exposure of Deoxynivalenol and Aflatoxin B1 on Neurotransmitter Secretion and Calcium Homeostasis in Mice

LIU Qin, CHU Xiaoyan, LIU Jinfang, ZHANG Yafei, ZHU Lei, ZHANG Mi, FENG Shibin, LI Yu, WU Jinjie, WANG Xichun

doi:10.3969/j.issn.1006-267x.2019.02.048

2019 , Vol. 31 >Issue 2: 912 - 921

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.02.048

Molecular Nutrition

Effects of Single and Combined Exposure of Deoxynivalenol and Aflatoxin B₁ on Neurotransmitter Secretion and Calcium Homeostasis in Mice

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College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received date: 2018-07-02

Online published: 2019-02-18

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Abstract

The aim of this experiment was to study the effects of single and combined exposure of deoxynivalenol (DON) and aflatoxin B₁ (AFB₁) on neurotransmitter secretion and calcium homeostasis in mice. Ninety-six Kunming male mice were randomly divided into 4 groups (24 mice each group). Each group was administered by gavage. Mice in control group were given physiological saline, mice in DON group were given 500 μg/kg DON, mice in AFB₁ group were given 200 μg/kg AFB₁ and mice in DON+AFB₁ group were given 200 μg/kg AFB₁+500 μg/kg DON. The trial was conducted for 45 days. On days 0 (before the trial), 15, 30 and 45, six mice were selected from each group and slaughtered, brain tissues were removed and the contents of neurotransmitters, calcium ion (Ca²⁺) and calmodulin (CaM) were detected by enzyme-linked immunosorbent assay (ELISA) kits. The content of CaM mRNA expression level was detected by quantitative real-time PCR (qRT-PCR) and the protein expression level of calmodulin-dependent protein kinase (CaMKⅡ) was detected by Western blotting. The results showed that compared with the control group on the each time point, the contents of 5-hydroxytryptamine (5-HT), glutamic acid (Glu), acetylcholine (ACH) were significantly increased (P<0.05 or P<0.01), while the contents of dopamine (DA), Ca²⁺ and CaM in DON+AFB₁ group were significantly decreased (P<0.01), as well as the CaM mRNA expression level (expect day 45) was significantly reduced (P<0.01). Meanwhile, the phosphorylated-CaMKⅡ (p-CaMKⅡ) protein expression level/CaMKⅡ protein expression level in DON+AFB₁ group was significantly higher than that in control group (P<0.01). Compared with the control group on the each time point, the contents of 5-HT and ACH in DON group and AFB₁ group were significantly increased (P<0.05 or P<0.01), while the content of Ca²⁺ was significantly decreased (P<0.01), and the CaM mRNA expression level (expect day 45) was significantly reduced (P<0.05 or P<0.01). However, the of p-CaMKⅡ protein expression level/CaMKⅡ protein expression level in DON group and AFB₁ group was significantly higher than that in control group (P<0.01). These results indicate that single and combined exposure of DON and AFB₁ all can cause disturbance of neurotransmitter secretion and imbalance of calcium homeostasis. The combined neurotoxic effect of DON and AFB₁ is greater than any of single exposure and they have synergistic effect.

Key words： deoxynivalenol; aflatoxin B₁; neurotransmitter; calmodulin; mice

Cite this article

LIU Qin, CHU Xiaoyan, LIU Jinfang, ZHANG Yafei, ZHU Lei, ZHANG Mi, FENG Shibin, LI Yu, WU Jinjie, WANG Xichun . Effects of Single and Combined Exposure of Deoxynivalenol and Aflatoxin B₁ on Neurotransmitter Secretion and Calcium Homeostasis in Mice[J]. Chinese Journal of Animal Nutrition, 2019 , 31(2) : 912 -921 . DOI: 10.3969/j.issn.1006-267x.2019.02.048

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