This experiment was conducted to investigate the effects of different nutrient level diets supplemented with enzymolytic soybean meal (ESM) on performance, egg quality, nutrient apparent availability and intestinal morphology of laying hens. A 3×2 two factorial design with 3 nutrient levels in diet, positive control (PC) with 11.22 MJ/kg metabolic energy (ME) and 15.5% crude protein (CP), negative control 1 (NC1) with 11.01 MJ/kg ME and 15.0% CP, negative control 2 (NC2) with 10.80 MJ/kg ME and 14.5% CP, and two ESM supplemental levels (0 and 5 g/kg) was used. A total of 1 200 Roman pink laying hens with 52 weeks old were randomly divided into 6 groups with 10 replicates per group and 20 laying hens per replicate. The experiment lasted for 12 weeks. The results showed as follows:1) with the decrease of nutrient levels in diet, average laying rate of laying hens had a decreasing tendency (P=0.07), and the ratio of feed to egg had an increasing tendency (P=0.06). With the increase of ESM supplemental levels, the ratio of feed to egg had a decreasing tendency (P=0.05), and qualified egg rate was significantly increased (P<0.05). 2) With the decrease of nutrient levels in diet, yolk color had a decreasing tendency (P=0.09). With the increase of ESM supplemental levels, yolk color was significantly increased (P<0.01). The interaction of nutrient levels in diet and ESM supplemental levels had significant effects on albumen height and Haugh unit (P<0.05), NC1 nutrient level diets supplemented with ESM significantly increased albumen height and Haugh unit (P<0.05). 3) Apparent availability of ether extract (EE) of laying hens in group PC was significantly higher than that of in groups NC1 and NC2 (P<0.05). With the increase of ESM supplemental levels, apparent availabilities of CP and EE were significantly increased (P<0.05). The interaction of nutrient levels in diet and ESM supplemental levels had significant effects on apparent availability of general energy of laying hens (P<0.05), PC nutrient level diets supplemented with ESM significantly increased apparent availability of total energy (P<0.05). 4) With the decrease of nutrient levels in diet, crypt depth of jejunum had an increasing tendency (P=0.06), the ratio of villous height to crypt depth (V/C) of jejunum was significantly decreased (P<0.05). With the increase of ESM supplemental levels, villous height of duodenum had an increasing tendency (P=0.09), V/C of duodenum was significantly increased (P<0.05). The interaction of nutrient levels in diet and ESM supplemental levels had significant effects on villous height and V/C of jejunum of laying hens (P<0.01), NC2 nutrient level diets supplemented with ESM significantly increased villous height and V/C of jejunum (P<0.05). In conclusion, diet supplemented with ESM can improve performance and egg quality of laying hens by improving intestinal morphology and apparent availabilities of CP and EE in diet, and can partial alleviate the negative influences by lower nutrient levels in diet.