This experiment was aimed to analyze the contents of 18 kinds of amino acids of rapeseed meals in 6 different areas from Hunan province, determine the amino acid standard ileal digestibility in rapeseed meals from 6 different areas in 14-day-old and 28-day-old broilers and establish the prediction equations of standard ileal digestibility of lysine (Lys), methionine (Met), tryptophan (Trp) and threonine (Thr). Seven hundred 1-day-old Arbor Acres (AA) broiler chickens were divided into two test stages according to their age. The first stage (7 to 14 days of age) selected 420 broiler chickens were randomly divided into 7 treatments with 10 replicates per treatment and 6 broilers per replicate, the second stage (21 to 28 days of age) selected 280 broiler chickens were randomly divided into 7 treatments with 10 replicates per treatment and 4 broilers per replicate. The 7 treatments in two stages were made up of 6 experimental diet groups designed by full substitute for rapeseed meal from 6 different areas and 1 nitrogen free diet group. Collected to the ileal chyme of 14-day-old and 28-day-old broilers to determine the standard ileal digestibility of amino acids in rapeseed meals and the prediction equations was established by regression analysis. The results showed as follows:the average fluctuation ranges of the standard ileal digestibility of amino acids in rapeseed meals in 14-day-old and 28-day-old broilers were 63.26% to 79.58% and 66.22% to 80.34%. For 14-day-old broilers, the prediction equations of standard ileal digestibility of Lys, Met, Trp and Thr in rapeseed meal were Lys=-90.81+4.562CP (R²=0.792, P<0.05), Met=110.644-2.810CF (R²=0.845, P<0.05), Trp=106.834-0.725NDF (R²=0.989, P<0.05) and Thr=-79.357+4.284CP (R²=0.715, P<0.05), respectively; for 28-day-old broilers, the prediction equations of standard ileal digestibility of Lys, Met, Trp and Thr in rapeseed meal were Lys=161.02-2.002NDF (R²=0.957, P<0.05), Met=144.269-1.521NDF (R²=0.831, P<0.05), Trp=112.053-0.824NDF (R²=0.999, P<0.05) and Thr=99.983-2.566CF (R²=0.712, P<0.05). In the above prediction equations, Lys was the standard ileal digestibility of Lys; Met was the standard ileal digestibility of Met; Trp was the standard ileal digestibility of Trp; Thr was the standard ileal digestibility of Thr; CP was the content of crude protein; CF was the content of crude fiber; NDF was the content of neutral detergent fiber. This experiment through analyzing the correlations between the contents of crude protein, crude fiber and neutral detergent fiber with the standard ileal digestibility of amino acids, established the prediction equations of standard ileal digestibility of Lys, Met, Trp and Thr in rapeseed meal from Hunan province of broilers aged 14 and 28 days, which can provide convenience for the efficient utilization of rapeseed meal in the diet of broiler chickens.