Molecular Nutrition

Effects of Fumarate on Ruminal Methane, Hydrogen Gas Production and Volatile Fatty Acid Composition Using an in Vitro Method

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  • 1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    3. College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received date: 2018-08-17

  Online published: 2019-03-18

Abstract

This study was conducted to investigate the effects of different supplemental levels of fumarate on methane and hydrogen gas production, volatile fatty acids composition and microbial population through in vitro fermentation. Rumen fluid was collected from three adult Xiangdong black goats fitted with permanent rumen cannulation. Using a 2×4 two-factor experimental design, corn flour and chicory were selected as substrates, fumarate had four (0, 3, 6 and 12 mmol/L) final concentrations, and blank groups were set for correcting the results, which were contained no substrate, just contained fumarate at concentrations of 0, 3, 6 or 12 mmol/L, respectively. Each group had 2 replicates, and were replicated 3 times. The 72 h in vitro ruminal incubation was performed using automatic fermentation incubation system. The results showed as follows:1) the 72 h gas production and dry matter degradation of corn flour were significantly higher than those of chicory (P<0.05). Fumarate supplementation had greater 72 h gas production (P<0.05); however, fumarate supplementation did not alter corrected 72 h gas production and dry matter degradation (P>0.05). There is no interaction between substrates and fumarate on 72 h gas production and dry matter degradation (P>0.05). 2) Hydrogen production and corrected hydrogen production of corn flour at 12 h were significantly higher than those of chicory (P<0.05), those at other time points did not have significant differences (P>0.05); methane production and corrected methane production of corn flour at each time points were significantly higher than those of chicory (P<0.05). Fumarate had no significant effect on methane production, corrected methane production, hydrogen production and corrected hydrogen production at each time points (P>0.05). There is no interaction between substrates and fumarate on hydrogen production and methane production (P>0.05). 3) Fumarate supplementation significantly increaed acetate, propionate and total volatile fatty acids concentrations (P<0.05), and significantly decreased pH and acetate to propionate ratio (P<0.05). But fumarate supplementation had no significant effect on corrected total volatile fatty acids concentrations(P>0.05). 4) Fumarate supplementation did not significantly affect microbial population (P>0.05). This study indicates that corn flour contains more fermentable carbohydrates than chicory, which help produce more gas and volatile fatty acids. Fumarate supplementation causes an increase in gas production and total volatile fatty acids concentration, which is mainly due to produce more gas and total volatile fatty acids during fumarate fermentation process, but has no significant effect on substrate self-degradation and microbial population.

Cite this article

WANG Rong, WEN Jiangnan, WANG Min, DENG Jinping, TAN Zhiliang . Effects of Fumarate on Ruminal Methane, Hydrogen Gas Production and Volatile Fatty Acid Composition Using an in Vitro Method[J]. Chinese Journal of Animal Nutrition, 2019 , 31(3) : 1198 -1209 . DOI: 10.3969/j.issn.1006-267x.2019.03.025

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