Effects of Nitrogen-Free Diet on Intestinal Digestive Enzyme Activities and Cecal Microflora of Rabbits

CAO Ningkun, WU Fengyang, CUI Jia, YANG Xinyu, SUN Ernu, WANG Zhaojun, GU Zilin, CHEN Baojiang

doi:10.3969/j.issn.1006-267x.2019.03.026

Chinese Journal of Animal Nutrition >

2019 , Vol. 31 >Issue 3: 1210 - 1219

DOI: https://doi.org/10.3969/j.issn.1006-267x.2019.03.026

Molecular Nutrition

Effects of Nitrogen-Free Diet on Intestinal Digestive Enzyme Activities and Cecal Microflora of Rabbits

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1. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071001, China;
2. Inner Mongolia Dongda Biological Technology Co., Ltd., Dalate 014300, China

Received date: 2018-08-22

Online published: 2019-03-18

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Abstract

This experiment was to investigate the effects of nitrogen-free diets on intestinal digestive enzyme activities and cecal microflora of rabbits, and to provide a reference for the study of endogenous nitrogen metabolism in rabbits. Twenty 1.5-year-old healthy Rex rabbits, meat rabbits and wool rabbits were selected, and the rabbits in each variety were randomly assigned to 2 groups based on gender. The rabbits in one group were fed a basal diet, while the rabbits in the other group were fed a nitrogen-free diet, each group had 10 replicates and each replicate had 1 rabbit. Both pretrial and formal trial periods lasted for 7 days. The results showed as follows:1) the intestinal amylase and cellulase activities of 3 kinds of rabbits in nitrogen-free diet group were higher than those in the basal diet group, and those of Rex rabbits, meat rabbits and wool rabbits increased by 8.00%, 4.08%, 20.69% and 12.45%, 12.19%, 9.47%, respectively, but the differences were not significant(P>0.05). The intestinal trypsin and lipase activities of 3 kinds of rabbits in nitrogen-free diet group were lower than those in the basal diet group, and those of Rex rabbits, meat rabbits and wool rabbits were 5.47%, 8.20%, 9.98% and 4.33%, 0.96%, 8.97%, respectively, but the differences were not significant (P>0.05). 2) Compared with the basal diet group, the cecal microbe diversity of Rex rabbits and meat rabbits trended to decrease, while that of wool rabbits trend to increase. For cecal microflora structure, the relative abundances of Bacteroides, Ruminococcaceae_NK4A214_group and Akkermansia of Rex rabbits in the nitrogen-free diet group were higher than those in the basal diet group, while the relative abundances of Ruminococcus_1, Lachnospiraceae_NK4A136_group were lower than those in the basal diet group. The relative abundances of Bacteroides and Akkermansia of meat rabbits in the nitrogen-free diet group were higher than those in the basal diet group, while the relative abundances of Ruminococcus_1, Lachnospiraceae_NK4A136_group, uncultured_bacterium_f_Porphyromonadaceae were lower than those in the basal diet group. The relative abundances of Bacteroides, Akkermansia and Lachnoclostridium of wool rabbits in the nitrogen-free diet group were higher than those in the basal diet group, while the relative abundances of uncultured_bacterium_f_Bacteroidales_S24-7_group and Ruminococcus_1 were lower than those in the basal diet group. In summary, nitrogen-free diet has some effects on intestinal digestive enzyme activities and cecal microflora of rabbits. These effects should be considered comprehensively when using the nitrogen-free diet method to study endogenous nitrogen metabolism in rabbits.

Key words： rabbits; nitrogen-free diet; digestive enzyme activities; cecal microbial diversity

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CAO Ningkun, WU Fengyang, CUI Jia, YANG Xinyu, SUN Ernu, WANG Zhaojun, GU Zilin, CHEN Baojiang . Effects of Nitrogen-Free Diet on Intestinal Digestive Enzyme Activities and Cecal Microflora of Rabbits[J]. Chinese Journal of Animal Nutrition, 2019 , 31(3) : 1210 -1219 . DOI: 10.3969/j.issn.1006-267x.2019.03.026

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